Zinc in PDB 8rbo: Cryo-Em Structure of Pyrococcus Furiosus Apo Form Rna Polymerase Contracted Clamp Conformation
Enzymatic activity of Cryo-Em Structure of Pyrococcus Furiosus Apo Form Rna Polymerase Contracted Clamp Conformation
All present enzymatic activity of Cryo-Em Structure of Pyrococcus Furiosus Apo Form Rna Polymerase Contracted Clamp Conformation:
2.7.7.6;
Other elements in 8rbo:
The structure of Cryo-Em Structure of Pyrococcus Furiosus Apo Form Rna Polymerase Contracted Clamp Conformation also contains other interesting chemical elements:
Zinc Binding Sites:
The binding sites of Zinc atom in the Cryo-Em Structure of Pyrococcus Furiosus Apo Form Rna Polymerase Contracted Clamp Conformation
(pdb code 8rbo). This binding sites where shown within
5.0 Angstroms radius around Zinc atom.
In total 5 binding sites of Zinc where determined in the
Cryo-Em Structure of Pyrococcus Furiosus Apo Form Rna Polymerase Contracted Clamp Conformation, PDB code: 8rbo:
Jump to Zinc binding site number:
1;
2;
3;
4;
5;
Zinc binding site 1 out
of 5 in 8rbo
Go back to
Zinc Binding Sites List in 8rbo
Zinc binding site 1 out
of 5 in the Cryo-Em Structure of Pyrococcus Furiosus Apo Form Rna Polymerase Contracted Clamp Conformation
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 1 of Cryo-Em Structure of Pyrococcus Furiosus Apo Form Rna Polymerase Contracted Clamp Conformation within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Zn1000
b:30.0
occ:1.00
|
NE2
|
A:HIS70
|
2.1
|
0.8
|
1.0
|
SG
|
A:CYS57
|
2.3
|
0.7
|
1.0
|
SG
|
A:CYS60
|
2.3
|
30.0
|
1.0
|
SG
|
A:CYS67
|
2.3
|
0.8
|
1.0
|
CE1
|
A:HIS70
|
3.0
|
0.8
|
1.0
|
CD2
|
A:HIS70
|
3.1
|
0.8
|
1.0
|
CB
|
A:CYS60
|
3.2
|
30.0
|
1.0
|
CB
|
A:CYS67
|
3.5
|
0.8
|
1.0
|
CB
|
A:CYS57
|
3.6
|
0.7
|
1.0
|
OG1
|
A:THR59
|
3.6
|
0.6
|
1.0
|
N
|
A:CYS60
|
3.6
|
30.0
|
1.0
|
CA
|
A:CYS60
|
3.8
|
30.0
|
1.0
|
N
|
A:GLY61
|
3.8
|
0.6
|
1.0
|
O
|
A:GLY61
|
4.0
|
0.6
|
1.0
|
C
|
A:CYS60
|
4.0
|
30.0
|
1.0
|
CA
|
A:CYS67
|
4.1
|
0.8
|
1.0
|
ND1
|
A:HIS70
|
4.1
|
0.8
|
1.0
|
CG
|
A:HIS70
|
4.2
|
0.8
|
1.0
|
CD
|
A:PRO68
|
4.4
|
0.8
|
1.0
|
CA
|
A:GLY49
|
4.7
|
0.8
|
1.0
|
C
|
A:THR59
|
4.7
|
0.6
|
1.0
|
CA
|
A:GLY61
|
4.8
|
0.6
|
1.0
|
C
|
A:GLY61
|
4.8
|
0.6
|
1.0
|
C
|
A:CYS67
|
4.8
|
0.8
|
1.0
|
N
|
A:PRO68
|
4.9
|
0.8
|
1.0
|
CA
|
A:CYS57
|
4.9
|
0.7
|
1.0
|
O
|
A:CYS60
|
4.9
|
30.0
|
1.0
|
CB
|
A:THR59
|
4.9
|
0.6
|
1.0
|
|
Zinc binding site 2 out
of 5 in 8rbo
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Zinc Binding Sites List in 8rbo
Zinc binding site 2 out
of 5 in the Cryo-Em Structure of Pyrococcus Furiosus Apo Form Rna Polymerase Contracted Clamp Conformation
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 2 of Cryo-Em Structure of Pyrococcus Furiosus Apo Form Rna Polymerase Contracted Clamp Conformation within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Zn1001
b:30.0
occ:1.00
|
SG
|
A:CYS97
|
2.3
|
0.8
|
1.0
|
SG
|
A:CYS100
|
2.3
|
0.7
|
1.0
|
SG
|
A:CYS147
|
2.3
|
0.8
|
1.0
|
SG
|
A:CYS144
|
2.3
|
0.7
|
1.0
|
CB
|
A:CYS144
|
3.1
|
0.7
|
1.0
|
CB
|
A:CYS97
|
3.2
|
0.8
|
1.0
|
CB
|
A:CYS147
|
3.6
|
0.8
|
1.0
|
OE2
|
A:GLU99
|
3.8
|
0.7
|
1.0
|
N
|
A:CYS147
|
3.9
|
0.8
|
1.0
|
N
|
A:CYS100
|
3.9
|
0.7
|
1.0
|
CB
|
A:CYS100
|
3.9
|
0.7
|
1.0
|
O
|
A:CYS147
|
4.2
|
0.8
|
1.0
|
CA
|
A:CYS147
|
4.2
|
0.8
|
1.0
|
CB
|
A:GLU99
|
4.5
|
0.7
|
1.0
|
CA
|
A:CYS100
|
4.5
|
0.7
|
1.0
|
CA
|
A:CYS144
|
4.6
|
0.7
|
1.0
|
CB
|
A:HIS146
|
4.6
|
0.7
|
1.0
|
CD2
|
A:HIS146
|
4.6
|
0.7
|
1.0
|
N
|
A:GLU99
|
4.7
|
0.7
|
1.0
|
C
|
A:CYS147
|
4.7
|
0.8
|
1.0
|
CA
|
A:CYS97
|
4.7
|
0.8
|
1.0
|
C
|
A:HIS146
|
4.8
|
0.7
|
1.0
|
C
|
A:GLU99
|
4.9
|
0.7
|
1.0
|
CA
|
A:GLU99
|
4.9
|
0.7
|
1.0
|
NE2
|
A:GLN151
|
4.9
|
0.7
|
1.0
|
CD
|
A:GLU99
|
4.9
|
0.7
|
1.0
|
N
|
A:HIS146
|
5.0
|
0.7
|
1.0
|
CG
|
A:HIS146
|
5.0
|
0.7
|
1.0
|
|
Zinc binding site 3 out
of 5 in 8rbo
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Zinc Binding Sites List in 8rbo
Zinc binding site 3 out
of 5 in the Cryo-Em Structure of Pyrococcus Furiosus Apo Form Rna Polymerase Contracted Clamp Conformation
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 3 of Cryo-Em Structure of Pyrococcus Furiosus Apo Form Rna Polymerase Contracted Clamp Conformation within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Zn1201
b:30.0
occ:1.00
|
CB
|
B:CYS1080
|
2.0
|
0.7
|
1.0
|
SG
|
B:CYS1062
|
2.3
|
30.0
|
1.0
|
SG
|
B:CYS1077
|
2.3
|
0.8
|
1.0
|
SG
|
B:CYS1059
|
2.3
|
0.8
|
1.0
|
SG
|
B:CYS1080
|
2.4
|
0.7
|
1.0
|
OD1
|
B:ASN1061
|
3.0
|
0.8
|
1.0
|
CB
|
B:CYS1062
|
3.3
|
30.0
|
1.0
|
CB
|
B:CYS1077
|
3.3
|
0.8
|
1.0
|
CA
|
B:CYS1080
|
3.5
|
0.7
|
1.0
|
CB
|
B:CYS1059
|
3.7
|
0.8
|
1.0
|
N
|
B:CYS1062
|
3.9
|
30.0
|
1.0
|
N
|
B:CYS1080
|
4.0
|
0.7
|
1.0
|
CG
|
B:ASN1061
|
4.0
|
0.8
|
1.0
|
CA
|
B:CYS1062
|
4.2
|
30.0
|
1.0
|
CB
|
B:GLU1082
|
4.2
|
0.7
|
1.0
|
C
|
B:CYS1080
|
4.3
|
0.7
|
1.0
|
ND2
|
B:ASN1061
|
4.4
|
0.8
|
1.0
|
CA
|
B:CYS1077
|
4.7
|
0.8
|
1.0
|
O
|
B:CYS1080
|
4.9
|
0.7
|
1.0
|
N
|
B:GLY1081
|
4.9
|
0.8
|
1.0
|
ND1
|
B:HIS1064
|
4.9
|
0.8
|
1.0
|
|
Zinc binding site 4 out
of 5 in 8rbo
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Zinc Binding Sites List in 8rbo
Zinc binding site 4 out
of 5 in the Cryo-Em Structure of Pyrococcus Furiosus Apo Form Rna Polymerase Contracted Clamp Conformation
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 4 of Cryo-Em Structure of Pyrococcus Furiosus Apo Form Rna Polymerase Contracted Clamp Conformation within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
N:Zn101
b:30.0
occ:1.00
|
SG
|
N:CYS45
|
2.3
|
0.6
|
1.0
|
SG
|
N:CYS10
|
2.3
|
0.6
|
1.0
|
SG
|
N:CYS44
|
2.3
|
0.6
|
1.0
|
SG
|
N:CYS7
|
2.3
|
0.6
|
1.0
|
N
|
N:CYS45
|
3.3
|
0.6
|
1.0
|
CB
|
N:CYS7
|
3.3
|
0.6
|
1.0
|
CB
|
N:CYS10
|
3.4
|
0.6
|
1.0
|
CB
|
N:CYS45
|
3.5
|
0.6
|
1.0
|
CA
|
N:CYS45
|
3.6
|
0.6
|
1.0
|
CB
|
N:CYS44
|
3.7
|
0.6
|
1.0
|
C
|
N:CYS44
|
3.7
|
0.6
|
1.0
|
N
|
N:CYS10
|
3.8
|
0.6
|
1.0
|
NH2
|
N:ARG42
|
4.2
|
0.5
|
1.0
|
CA
|
N:CYS10
|
4.2
|
0.6
|
1.0
|
O
|
N:CYS44
|
4.3
|
0.6
|
1.0
|
CA
|
N:CYS44
|
4.3
|
0.6
|
1.0
|
NE
|
N:ARG42
|
4.6
|
0.5
|
1.0
|
N
|
N:GLY11
|
4.6
|
0.5
|
1.0
|
CB
|
N:THR9
|
4.7
|
0.5
|
1.0
|
CA
|
N:CYS7
|
4.7
|
0.6
|
1.0
|
C
|
N:CYS10
|
4.8
|
0.6
|
1.0
|
CZ
|
N:ARG42
|
4.9
|
0.5
|
1.0
|
C
|
N:THR9
|
4.9
|
0.5
|
1.0
|
N
|
N:CYS44
|
5.0
|
0.6
|
1.0
|
N
|
N:LYS12
|
5.0
|
0.6
|
1.0
|
N
|
N:THR9
|
5.0
|
0.5
|
1.0
|
|
Zinc binding site 5 out
of 5 in 8rbo
Go back to
Zinc Binding Sites List in 8rbo
Zinc binding site 5 out
of 5 in the Cryo-Em Structure of Pyrococcus Furiosus Apo Form Rna Polymerase Contracted Clamp Conformation
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 5 of Cryo-Em Structure of Pyrococcus Furiosus Apo Form Rna Polymerase Contracted Clamp Conformation within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
P:Zn101
b:30.0
occ:1.00
|
SG
|
P:CYS8
|
2.3
|
86.8
|
1.0
|
SG
|
P:CYS11
|
2.3
|
84.7
|
1.0
|
SG
|
P:CYS30
|
2.3
|
30.0
|
1.0
|
SG
|
P:CYS27
|
2.3
|
79.7
|
1.0
|
CB
|
P:CYS27
|
3.2
|
79.7
|
1.0
|
CB
|
P:CYS8
|
3.2
|
86.8
|
1.0
|
CB
|
P:CYS30
|
3.6
|
30.0
|
1.0
|
CB
|
P:CYS11
|
3.8
|
84.7
|
1.0
|
N
|
P:CYS30
|
3.9
|
30.0
|
1.0
|
N
|
P:CYS11
|
4.0
|
84.7
|
1.0
|
CA
|
P:CYS30
|
4.3
|
30.0
|
1.0
|
CB
|
P:SER32
|
4.4
|
83.0
|
1.0
|
CA
|
P:CYS11
|
4.5
|
84.7
|
1.0
|
CA
|
P:CYS27
|
4.7
|
79.7
|
1.0
|
CA
|
P:CYS8
|
4.7
|
86.8
|
1.0
|
CB
|
P:TYR29
|
4.7
|
81.8
|
1.0
|
N
|
P:SER32
|
4.8
|
83.0
|
1.0
|
C
|
P:CYS30
|
4.8
|
30.0
|
1.0
|
C
|
P:TYR29
|
5.0
|
81.8
|
1.0
|
N
|
P:GLY31
|
5.0
|
80.6
|
1.0
|
CB
|
P:LYS10
|
5.0
|
83.7
|
1.0
|
|
Reference:
D.M.Tarau,
F.Grunberger,
M.Pilsl,
R.Reichelt,
F.B.Heiss,
S.Koenig,
H.Urlaub,
W.Hausner,
C.Engel,
D.Grohmann.
Structural Basis of Archaeal Rna Polymerase Transcription Elongation and SPT4/5 Recruitment Nucleic Acids Res. 2024.
ISSN: ESSN 1362-4962
Page generated: Thu Oct 31 10:26:48 2024
|