Zinc in PDB 8ppv: Intermediate Conformer of Pyrococcus Abyssi Dna Polymerase D (Pold) Bound to A Primer/Template Substrate Containing Three Consecutive Mismatches

Enzymatic activity of Intermediate Conformer of Pyrococcus Abyssi Dna Polymerase D (Pold) Bound to A Primer/Template Substrate Containing Three Consecutive Mismatches

All present enzymatic activity of Intermediate Conformer of Pyrococcus Abyssi Dna Polymerase D (Pold) Bound to A Primer/Template Substrate Containing Three Consecutive Mismatches:
2.7.7.7; 3.1.11.1;

Other elements in 8ppv:

The structure of Intermediate Conformer of Pyrococcus Abyssi Dna Polymerase D (Pold) Bound to A Primer/Template Substrate Containing Three Consecutive Mismatches also contains other interesting chemical elements:

Magnesium (Mg) 3 atoms

Zinc Binding Sites:

The binding sites of Zinc atom in the Intermediate Conformer of Pyrococcus Abyssi Dna Polymerase D (Pold) Bound to A Primer/Template Substrate Containing Three Consecutive Mismatches (pdb code 8ppv). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 3 binding sites of Zinc where determined in the Intermediate Conformer of Pyrococcus Abyssi Dna Polymerase D (Pold) Bound to A Primer/Template Substrate Containing Three Consecutive Mismatches, PDB code: 8ppv:
Jump to Zinc binding site number: 1; 2; 3;

Zinc binding site 1 out of 3 in 8ppv

Go back to Zinc Binding Sites List in 8ppv
Zinc binding site 1 out of 3 in the Intermediate Conformer of Pyrococcus Abyssi Dna Polymerase D (Pold) Bound to A Primer/Template Substrate Containing Three Consecutive Mismatches


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Intermediate Conformer of Pyrococcus Abyssi Dna Polymerase D (Pold) Bound to A Primer/Template Substrate Containing Three Consecutive Mismatches within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Zn1301

b:112.9
occ:1.00
SG B:CYS718 2.3 63.2 1.0
SG B:CYS709 2.3 76.8 1.0
SG B:CYS706 2.3 60.6 1.0
SG B:CYS721 2.3 68.1 1.0
CB B:CYS718 3.1 46.1 1.0
CB B:CYS706 3.2 51.9 1.0
CB B:CYS709 4.0 67.8 1.0
CB B:CYS721 4.1 60.5 1.0
N B:CYS709 4.2 68.2 1.0
N B:CYS721 4.4 61.8 1.0
OG1 B:THR723 4.4 52.1 1.0
CA B:CYS718 4.5 42.9 1.0
CA B:CYS706 4.6 45.2 1.0
CA B:CYS709 4.7 68.8 1.0
CB B:HIS711 4.7 45.7 1.0
CB B:ASN720 4.8 57.1 1.0
N B:GLY710 4.8 58.8 1.0
CA B:CYS721 4.8 60.6 1.0
CB B:LYS708 4.9 66.1 1.0
N B:HIS711 4.9 49.0 1.0

Zinc binding site 2 out of 3 in 8ppv

Go back to Zinc Binding Sites List in 8ppv
Zinc binding site 2 out of 3 in the Intermediate Conformer of Pyrococcus Abyssi Dna Polymerase D (Pold) Bound to A Primer/Template Substrate Containing Three Consecutive Mismatches


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Intermediate Conformer of Pyrococcus Abyssi Dna Polymerase D (Pold) Bound to A Primer/Template Substrate Containing Three Consecutive Mismatches within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Zn1302

b:135.4
occ:1.00
SG B:CYS750 2.3 73.7 1.0
SG B:CYS734 2.3 79.4 1.0
SG B:CYS731 2.3 77.9 1.0
SG B:CYS753 2.3 91.0 1.0
CB B:CYS750 3.2 72.5 1.0
CB B:CYS731 3.3 69.8 1.0
CB B:CYS753 3.5 84.0 1.0
CB B:CYS734 3.6 77.7 1.0
N B:CYS734 3.9 75.1 1.0
N B:CYS753 4.1 84.0 1.0
CA B:CYS734 4.3 77.2 1.0
CA B:CYS753 4.5 82.0 1.0
CB B:LYS752 4.6 85.0 1.0
CB B:ARG733 4.6 82.0 1.0
C B:LYS752 4.6 84.0 1.0
CA B:CYS750 4.7 74.2 1.0
CA B:CYS731 4.7 67.5 1.0
C B:ARG733 4.9 79.1 1.0
CD B:LYS752 5.0 82.1 1.0
CA B:LYS752 5.0 84.7 1.0
C B:CYS734 5.0 78.2 1.0

Zinc binding site 3 out of 3 in 8ppv

Go back to Zinc Binding Sites List in 8ppv
Zinc binding site 3 out of 3 in the Intermediate Conformer of Pyrococcus Abyssi Dna Polymerase D (Pold) Bound to A Primer/Template Substrate Containing Three Consecutive Mismatches


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Intermediate Conformer of Pyrococcus Abyssi Dna Polymerase D (Pold) Bound to A Primer/Template Substrate Containing Three Consecutive Mismatches within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Zn1303

b:131.8
occ:1.00
CB B:CYS1142 2.1 90.0 1.0
SG B:CYS1142 2.3 92.2 1.0
SG B:CYS1123 2.3 82.0 1.0
SG B:CYS1139 2.3 88.5 1.0
CB B:CYS1139 3.2 83.5 1.0
SG B:CYS1126 3.3 81.5 1.0
CB B:CYS1123 3.3 71.7 1.0
CA B:CYS1142 3.6 85.9 1.0
CB B:CYS1126 4.2 75.8 1.0
N B:CYS1142 4.3 86.7 1.0
N B:CYS1126 4.4 77.4 1.0
C B:CYS1142 4.4 86.2 1.0
CA B:CYS1139 4.6 82.8 1.0
O B:PRO1140 4.7 83.4 1.0
CG2 B:THR1128 4.7 69.8 1.0
O B:VAL1141 4.7 84.4 1.0
CA B:CYS1123 4.7 71.3 1.0
O B:CYS1139 4.7 84.7 1.0
N B:GLY1143 4.8 88.5 1.0
C B:VAL1141 4.8 86.0 1.0
CB B:LYS1125 4.8 79.4 1.0
C B:CYS1139 4.9 83.2 1.0
N B:GLY1144 5.0 89.2 1.0
CA B:CYS1126 5.0 78.2 1.0

Reference:

L.Betancurt-Anzola, K.M.Zatopek, M.Martinez-Carranza, A.F.Gardner, L.Sauguet. Molecular Basis For Proof-Reading By Family-D Dna Polymerases To Be Published.
Page generated: Thu Oct 31 09:45:19 2024

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