Zinc in PDB 8ot0: Sars-Cov-2 NSP10-16 Methyltransferase in Complex with Mta and Glycine

All present enzymatic activity of Sars-Cov-2 NSP10-16 Methyltransferase in Complex with Mta and Glycine:
2.1.1.57;

The structure of Sars-Cov-2 NSP10-16 Methyltransferase in Complex with Mta and Glycine, PDB code: 8ot0 was solved by V.Kremling, J.Sprenger, D.Oberthuer, S.Falke, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	43.77 / 2.21
Space group	P 31 2 1
Cell size a, b, c (Å), α, β, γ (°)	167.102, 167.102, 51.379, 90, 90, 120
R / Rfree (%)	18.3 / 21

The binding sites of Zinc atom in the Sars-Cov-2 NSP10-16 Methyltransferase in Complex with Mta and Glycine (pdb code 8ot0). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Sars-Cov-2 NSP10-16 Methyltransferase in Complex with Mta and Glycine, PDB code: 8ot0:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Sars-Cov-2 NSP10-16 Methyltransferase in Complex with Mta and Glycine


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Sars-Cov-2 NSP10-16 Methyltransferase in Complex with Mta and Glycine within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn4401 b:41.3 occ:1.00 NE2 B:HIS4336 2.1 51.4 1.0 SG B:CYS4327 2.3 38.8 1.0 SG B:CYS4343 2.3 45.8 1.0 SG B:CYS4330 2.3 40.9 1.0 CE1 B:HIS4336 3.0 48.5 1.0 CB B:CYS4327 3.1 37.4 1.0 CD2 B:HIS4336 3.1 49.2 1.0 CB B:CYS4343 3.3 52.6 1.0 CB B:CYS4330 3.4 37.7 1.0 N B:CYS4330 3.6 35.5 1.0 CA B:CYS4330 3.9 34.0 1.0 ND1 B:HIS4336 4.1 50.1 1.0 CG B:HIS4336 4.2 65.0 1.0 O B:HOH4547 4.4 59.2 1.0 CA B:CYS4343 4.4 56.2 1.0 CA B:CYS4327 4.5 35.5 1.0 CB B:TYR4329 4.5 42.8 1.0 C B:TYR4329 4.6 48.7 1.0 CB B:LYS4346 4.7 33.1 1.0 C B:CYS4327 4.9 37.8 1.0 O B:CYS4327 4.9 35.7 1.0 N B:LYS4346 5.0 38.7 1.0 CA B:TYR4329 5.0 43.8 1.0 N B:TYR4329 5.0 40.8 1.0

Zinc binding site 2 out of 2 in the Sars-Cov-2 NSP10-16 Methyltransferase in Complex with Mta and Glycine


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Sars-Cov-2 NSP10-16 Methyltransferase in Complex with Mta and Glycine within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn4402 b:68.1 occ:1.00 SG B:CYS4370 2.3 62.3 1.0 SG B:CYS4381 2.3 62.5 1.0 SG B:CYS4383 2.4 75.7 1.0 SG B:CYS4373 2.4 56.2 1.0 CB B:CYS4370 3.2 57.4 1.0 CB B:CYS4383 3.3 64.5 1.0 CB B:CYS4373 3.4 57.6 1.0 CB B:CYS4381 3.6 65.6 1.0 O B:HOH4503 3.6 66.2 1.0 N B:CYS4373 3.8 57.7 1.0 N B:CYS4383 4.1 77.8 1.0 CA B:CYS4381 4.1 64.7 1.0 CA B:CYS4373 4.2 65.6 1.0 CA B:CYS4383 4.3 76.5 1.0 N B:SER4382 4.4 69.6 1.0 C B:CYS4381 4.6 72.0 1.0 CA B:CYS4370 4.6 58.5 1.0 CB B:VAL4372 4.7 65.5 1.0 NZ B:LYS4377 4.7 72.5 1.0 C B:VAL4372 4.9 61.9 1.0 C B:CYS4373 4.9 59.1 1.0

V.Kremling, J.Sprenger, D.Oberthuer, S.Falke, O.Yefanov, M.Galchenkova, P.Middendorf, Y.Garcia, C.Ehr, A.Kiene, B.Klopprogge, H.Chapman. Crystal Structures of Tubercidin and Adenosine Bound to the Active Site of the Sars-Cov-2 Methyltransferase NSP10-16 To Be Published.