Zinc in PDB 8jpw: Crystal Structure of Single-Chain L-Glutamate Oxidase Mutant From Streptomyces Sp. X-119-6

The structure of Crystal Structure of Single-Chain L-Glutamate Oxidase Mutant From Streptomyces Sp. X-119-6, PDB code: 8jpw was solved by H.Yamaguchi, K.Takahashi, M.Tatsumi, U.Tagami, T.Mizukoshi, H.Miyano, M.Sugiki, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	49.32 / 2.66
Space group	I 4 2 2
Cell size a, b, c (Å), α, β, γ (°)	158.4, 158.4, 138.235, 90, 90, 90
R / Rfree (%)	25.9 / 30.5

The binding sites of Zinc atom in the Crystal Structure of Single-Chain L-Glutamate Oxidase Mutant From Streptomyces Sp. X-119-6 (pdb code 8jpw). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of Single-Chain L-Glutamate Oxidase Mutant From Streptomyces Sp. X-119-6, PDB code: 8jpw:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of Single-Chain L-Glutamate Oxidase Mutant From Streptomyces Sp. X-119-6


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Single-Chain L-Glutamate Oxidase Mutant From Streptomyces Sp. X-119-6 within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn702 b:99.6 occ:1.00 O A:HOH820 2.2 59.7 1.0 CE1 A:HIS110 2.3 72.2 1.0 OE1 A:GLU346 2.6 68.5 1.0 NE2 A:HIS110 2.7 71.0 1.0 NZ A:LYS112 3.1 96.5 1.0 ND1 A:HIS110 3.5 70.4 1.0 CD A:GLU346 3.7 72.4 1.0 CE A:LYS112 4.0 96.2 1.0 CD2 A:HIS110 4.1 71.2 1.0 OE2 A:GLU346 4.2 61.3 1.0 CG A:HIS110 4.4 73.3 1.0 CD A:ARG342 4.6 66.2 1.0 O A:HOH933 4.6 52.0 1.0 CG A:ARG101 4.9 63.0 1.0 NH1 A:ARG342 4.9 64.7 1.0 CG A:GLU346 5.0 62.4 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of Single-Chain L-Glutamate Oxidase Mutant From Streptomyces Sp. X-119-6


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of Single-Chain L-Glutamate Oxidase Mutant From Streptomyces Sp. X-119-6 within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn703 b:30.0 occ:1.00 OD1 A:ASP167 2.2 57.6 1.0 CG A:ASP167 3.0 56.6 1.0 OD2 A:ASP167 3.2 60.6 1.0 O A:HOH916 3.5 49.8 1.0 CB A:ASP167 4.3 52.6 1.0

H.Yamaguchi, K.Takahashi, M.Tatsumi, U.Tagami, T.Mizukoshi, H.Miyano, M.Sugiki. Development of A Novel Single-Chain L-Glutamate Oxidase From Streptomyces Sp. X-119-6 By Inserting Flexible Linkers Enzyme.Microb.Technol. V. 170 10287 2023.
ISSN: ISSN 0141-0229
DOI: 10.1016/J.ENZMICTEC.2023.110287