Zinc in PDB 8igs: Cryo-Em Structure of Rnap-Promoter Open Complex at Lambda Promoter Pre

All present enzymatic activity of Cryo-Em Structure of Rnap-Promoter Open Complex at Lambda Promoter Pre:
2.7.7.6;

The structure of Cryo-Em Structure of Rnap-Promoter Open Complex at Lambda Promoter Pre also contains other interesting chemical elements:

Magnesium

(Mg)

1 atom

The binding sites of Zinc atom in the Cryo-Em Structure of Rnap-Promoter Open Complex at Lambda Promoter Pre (pdb code 8igs). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Cryo-Em Structure of Rnap-Promoter Open Complex at Lambda Promoter Pre, PDB code: 8igs:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Cryo-Em Structure of Rnap-Promoter Open Complex at Lambda Promoter Pre


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Cryo-Em Structure of Rnap-Promoter Open Complex at Lambda Promoter Pre within 5.0Å range: probe atom residue distance (Å) B Occ J:Zn2002 b:248.8 occ:1.00 SG J:CYS70 2.3 172.5 1.0 SG J:CYS85 2.3 176.1 1.0 SG J:CYS72 2.3 177.6 1.0 SG J:CYS88 2.3 168.5 1.0 CB J:CYS85 3.0 169.8 1.0 CB J:CYS72 3.1 168.7 1.0 N J:CYS72 3.6 168.7 1.0 CB J:CYS88 3.8 165.2 1.0 CA J:CYS72 3.9 168.2 1.0 N J:CYS88 4.0 168.6 1.0 CB J:CYS70 4.0 169.4 1.0 CA J:CYS88 4.5 166.4 1.0 N J:GLY73 4.5 164.7 1.0 CA J:CYS85 4.5 169.1 1.0 N J:LEU71 4.6 145.5 1.0 C J:CYS72 4.7 169.0 1.0 CB J:LYS87 4.8 171.6 1.0 CB J:LYS74 4.8 167.4 1.0 C J:LEU71 4.8 145.2 1.0 N J:LYS74 4.8 170.1 1.0 CD1 J:TYR75 4.8 167.1 1.0 C J:LYS87 4.8 173.4 1.0 C J:CYS70 4.8 170.2 1.0 N J:LYS87 4.9 172.4 1.0 N J:GLY89 4.9 153.3 1.0 N J:GLU86 4.9 174.6 1.0 C J:CYS88 5.0 163.4 1.0

Zinc binding site 2 out of 2 in the Cryo-Em Structure of Rnap-Promoter Open Complex at Lambda Promoter Pre


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Cryo-Em Structure of Rnap-Promoter Open Complex at Lambda Promoter Pre within 5.0Å range: probe atom residue distance (Å) B Occ J:Zn2003 b:167.4 occ:1.00 SG J:CYS895 2.3 108.7 1.0 SG J:CYS898 2.3 110.0 1.0 SG J:CYS814 2.3 113.7 1.0 SG J:CYS888 2.3 91.0 1.0 CB J:CYS898 3.0 97.7 1.0 CB J:CYS814 3.0 98.1 1.0 CB J:CYS888 3.4 87.2 1.0 CB J:CYS895 3.5 93.0 1.0 CA J:CYS888 3.8 88.2 1.0 NH1 J:ARG883 4.0 97.6 1.0 N J:CYS814 4.0 94.4 1.0 N J:CYS895 4.1 77.7 1.0 CA J:CYS898 4.1 89.7 1.0 N J:CYS898 4.1 88.0 1.0 CA J:CYS814 4.1 93.8 1.0 CA J:CYS895 4.3 83.9 1.0 N J:ASP889 4.4 108.5 1.0 OG1 J:THR890 4.5 100.6 1.0 CG2 J:THR816 4.5 103.5 1.0 O J:CYS895 4.6 79.3 1.0 C J:CYS888 4.6 94.3 1.0 C J:CYS895 4.8 79.5 1.0 N J:CYS888 4.9 89.9 1.0 CD J:ARG883 5.0 95.1 1.0

M.Zhao, B.Gao, A.Wen, Y.Feng, Y.Lu. Structural Basis of Lamdacii-Dependent Transcription Activation Structure 2023.
ISSN: ISSN 0969-2126