Zinc in PDB 8i16: Crystal Structure of the Selenomethionine (Semet)-Derived CAS12G (D513A) Mutant

The structure of Crystal Structure of the Selenomethionine (Semet)-Derived CAS12G (D513A) Mutant, PDB code: 8i16 was solved by B.Zhang, J.Chen, Y.M.Ye, S.Y.Ouyang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	62.86 / 2.24
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	64.07, 95.12, 167.51, 90, 90, 90
R / Rfree (%)	22.4 / 26.8

The binding sites of Zinc atom in the Crystal Structure of the Selenomethionine (Semet)-Derived CAS12G (D513A) Mutant (pdb code 8i16). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of the Selenomethionine (Semet)-Derived CAS12G (D513A) Mutant, PDB code: 8i16:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of the Selenomethionine (Semet)-Derived CAS12G (D513A) Mutant


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of the Selenomethionine (Semet)-Derived CAS12G (D513A) Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn801 b:63.1 occ:1.00 SG A:CYS228 2.2 57.7 1.0 ND1 A:HIS335 2.2 52.7 1.0 SG A:CYS339 2.2 58.6 1.0 CB A:CYS240 2.4 76.6 1.0 CB A:CYS228 3.0 57.2 1.0 CA A:CYS240 3.0 70.5 1.0 CE1 A:HIS335 3.0 51.6 1.0 CB A:CYS339 3.0 54.9 1.0 SG A:CYS240 3.1 91.7 1.0 CG A:HIS335 3.3 56.0 1.0 CB A:HIS335 3.7 61.4 1.0 N A:CYS240 4.0 62.6 1.0 CA A:HIS335 4.0 67.2 1.0 N A:CYS228 4.1 53.8 1.0 C A:CYS240 4.1 68.1 1.0 CD1 A:LEU233 4.1 69.4 1.0 CA A:CYS228 4.2 57.9 1.0 N A:GLN241 4.2 70.5 1.0 NE2 A:HIS335 4.2 56.1 1.0 CD2 A:HIS335 4.4 51.7 1.0 CA A:CYS339 4.4 52.7 1.0 N A:THR336 4.5 60.9 1.0 CD2 A:TYR242 4.7 66.6 1.0 OG1 A:THR336 4.8 64.9 1.0 C A:HIS335 4.8 61.6 1.0 C A:ASN239 5.0 68.2 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of the Selenomethionine (Semet)-Derived CAS12G (D513A) Mutant


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of the Selenomethionine (Semet)-Derived CAS12G (D513A) Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn802 b:80.9 occ:1.00 ND1 A:HIS248 2.2 73.9 1.0 SG A:CYS323 2.3 78.9 1.0 SG A:CYS252 2.5 75.2 1.0 SG A:CYS330 2.7 100.8 1.0 CB A:CYS330 2.7 78.4 1.0 CB A:CYS323 2.8 71.6 1.0 CG A:HIS248 3.0 70.7 1.0 CB A:CYS252 3.0 65.1 1.0 CB A:HIS248 3.1 73.0 1.0 CE1 A:HIS248 3.3 81.9 1.0 CA A:CYS330 3.6 83.1 1.0 CA A:HIS248 3.8 80.2 1.0 N A:CYS323 3.8 60.1 1.0 CA A:CYS323 4.0 60.7 1.0 N A:SER249 4.1 68.4 1.0 CD2 A:HIS248 4.2 74.2 1.0 NE2 A:HIS248 4.3 74.6 1.0 C A:HIS248 4.5 70.3 1.0 CA A:CYS252 4.5 64.4 1.0 N A:CYS330 4.5 82.9 1.0 C A:CYS330 4.7 84.3 1.0 CD2 A:PHE332 4.7 58.1 1.0 N A:GLU331 4.8 82.2 1.0 O A:ASN325 4.9 92.2 1.0

B.Zhang, J.Chen, Y.M.Ye, S.Y.Ouyang. Crystal Structure of the Selenomethionine (Semet)-Derived CAS12G (D513A) Mutant To Be Published.