Zinc in PDB 8hkh: Crystal Structure of the Lc/A1-DARPIN18 Complex

The structure of Crystal Structure of the Lc/A1-DARPIN18 Complex, PDB code: 8hkh was solved by Y.Wu, O.Leka, R.Kammerer, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	49.19 / 2.70
Space group	P 1
Cell size a, b, c (Å), α, β, γ (°)	59.56, 59.63, 102.18, 74.98, 89.9, 73.15
R / Rfree (%)	26.2 / 30.6

The binding sites of Zinc atom in the Crystal Structure of the Lc/A1-DARPIN18 Complex (pdb code 8hkh). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of the Lc/A1-DARPIN18 Complex, PDB code: 8hkh:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of the Lc/A1-DARPIN18 Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of the Lc/A1-DARPIN18 Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn501 b:43.3 occ:1.00 OE1 A:GLU262 2.0 41.5 1.0 OE2 A:GLU262 2.0 41.5 1.0 NE2 A:HIS227 2.0 27.6 1.0 NE2 A:HIS223 2.1 38.9 1.0 CD A:GLU262 2.2 38.8 1.0 CD2 A:HIS227 2.8 31.4 1.0 HD2 A:HIS227 2.9 37.7 1.0 CD2 A:HIS223 3.0 35.4 1.0 CE1 A:HIS223 3.0 41.9 1.0 CE1 A:HIS227 3.2 33.5 1.0 HD2 A:HIS223 3.2 42.5 1.0 HE1 A:HIS223 3.2 50.3 1.0 HH A:TYR366 3.3 58.2 1.0 HE1 A:HIS227 3.5 40.2 1.0 N D:HIS7 3.6 31.6 1.0 CG A:GLU262 3.7 44.5 1.0 HE1 A:TYR366 3.8 56.7 1.0 OE2 A:GLU224 4.0 44.3 1.0 OE1 A:GLU224 4.1 46.4 1.0 CG A:HIS227 4.1 35.2 1.0 HG2 A:GLU262 4.1 53.4 1.0 ND1 A:HIS223 4.1 41.1 1.0 OH A:TYR366 4.1 48.5 1.0 CG A:HIS223 4.1 35.6 1.0 ND1 A:HIS227 4.2 34.1 1.0 HA A:GLU262 4.2 49.1 1.0 HB3 A:GLU262 4.2 42.5 1.0 HG3 A:GLU262 4.2 53.4 1.0 HA D:HIS7 4.2 51.0 1.0 CD A:GLU224 4.4 48.3 1.0 HG21 A:THR265 4.5 36.4 1.0 CB A:GLU262 4.5 35.4 1.0 CA D:HIS7 4.5 42.5 1.0 CE1 A:TYR366 4.5 47.2 1.0 HB A:THR265 4.7 40.4 1.0 CZ A:TYR366 4.8 50.8 1.0 CA A:GLU262 4.9 40.9 1.0 HD1 A:HIS223 4.9 49.4 1.0 HA A:GLU224 5.0 42.9 1.0 HD1 A:HIS227 5.0 40.9 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of the Lc/A1-DARPIN18 Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of the Lc/A1-DARPIN18 Complex within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn501 b:41.4 occ:1.00 OE1 B:GLU262 2.0 41.0 1.0 OE2 B:GLU262 2.0 28.7 1.0 NE2 B:HIS227 2.0 32.0 1.0 NE2 B:HIS223 2.1 48.1 1.0 CD B:GLU262 2.2 33.4 1.0 CD2 B:HIS227 2.9 25.7 1.0 CD2 B:HIS223 3.0 29.3 1.0 HD2 B:HIS227 3.0 30.8 1.0 HD2 B:HIS223 3.1 35.1 1.0 CE1 B:HIS227 3.1 28.4 1.0 CE1 B:HIS223 3.1 32.4 1.0 N C:HIS7 3.4 28.3 1.0 HE1 B:HIS227 3.4 34.1 1.0 HE1 B:HIS223 3.4 38.9 1.0 CG B:GLU262 3.7 46.7 1.0 HH B:TYR366 3.8 51.1 1.0 HA C:HIS7 3.9 63.8 1.0 OE1 B:GLU224 4.1 47.0 1.0 OE2 B:GLU224 4.1 42.1 1.0 CG B:HIS227 4.1 30.1 1.0 HG2 B:GLU262 4.1 56.0 1.0 CG B:HIS223 4.1 25.9 1.0 ND1 B:HIS227 4.2 38.7 1.0 ND1 B:HIS223 4.2 35.3 1.0 HE1 B:TYR366 4.2 53.8 1.0 HG3 B:GLU262 4.2 56.0 1.0 CA C:HIS7 4.2 53.2 1.0 HB3 B:GLU262 4.2 53.6 1.0 HA B:GLU262 4.3 46.7 1.0 CD B:GLU224 4.4 44.3 1.0 HG21 B:THR265 4.4 43.4 1.0 OH B:TYR366 4.5 42.6 1.0 CB B:GLU262 4.5 44.7 1.0 HB B:THR265 4.8 39.1 1.0 HG22 B:THR265 4.8 43.4 1.0 CE1 B:TYR366 4.9 44.9 1.0 CA B:GLU262 4.9 38.9 1.0 HD1 B:HIS227 5.0 46.4 1.0 HA B:GLU224 5.0 52.6 1.0 HD1 B:HIS223 5.0 42.4 1.0

O.Leka, Y.Wu, R.Kammerer, X.Li, A.Plueckthun. A Darpin Increases the Catalytic Activity of Bont/A1 To Be Published.