Zinc in PDB 8fim: Structure of APOBEC3A (E72A Inactive Mutant) in Complex with Ttc- Hairpin Dna Substrate

All present enzymatic activity of Structure of APOBEC3A (E72A Inactive Mutant) in Complex with Ttc- Hairpin Dna Substrate:
3.5.4.38;

The structure of Structure of APOBEC3A (E72A Inactive Mutant) in Complex with Ttc- Hairpin Dna Substrate, PDB code: 8fim was solved by S.Harjes, G.B.Jameson, E.Harjes, V.V.Filichev, H.M.Kurup, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	47.99 / 2.22
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	53.917, 56.904, 91.549, 90, 103.57, 90
R / Rfree (%)	23.8 / 27.7

The structure of Structure of APOBEC3A (E72A Inactive Mutant) in Complex with Ttc- Hairpin Dna Substrate also contains other interesting chemical elements:

Chlorine

(Cl)

2 atoms

The binding sites of Zinc atom in the Structure of APOBEC3A (E72A Inactive Mutant) in Complex with Ttc- Hairpin Dna Substrate (pdb code 8fim). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Structure of APOBEC3A (E72A Inactive Mutant) in Complex with Ttc- Hairpin Dna Substrate, PDB code: 8fim:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Structure of APOBEC3A (E72A Inactive Mutant) in Complex with Ttc- Hairpin Dna Substrate


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Structure of APOBEC3A (E72A Inactive Mutant) in Complex with Ttc- Hairpin Dna Substrate within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn202 b:53.0 occ:0.50 ND1 A:HIS70 1.8 52.7 1.0 SG A:CYS106 2.0 80.9 1.0 CL A:CL203 2.1 69.0 1.0 SG A:CYS101 2.5 69.1 1.0 CE1 A:HIS70 2.8 54.4 1.0 CG A:HIS70 2.9 53.0 1.0 CB A:CYS106 3.1 76.9 1.0 CB A:CYS101 3.2 63.0 1.0 CB A:HIS70 3.3 51.7 1.0 N A:CYS101 3.9 60.0 1.0 NE2 A:HIS70 3.9 54.8 1.0 CD2 A:HIS70 4.0 54.5 1.0 CA A:CYS101 4.0 63.4 1.0 CB A:TRP104 4.4 102.6 0.5 CB A:TRP104 4.4 109.3 0.5 CA A:CYS106 4.4 77.0 1.0 C A:CYS101 4.6 65.9 1.0 O A:HOH306 4.6 47.7 1.0 O A:CYS101 4.6 63.5 1.0 N A:CYS106 4.6 85.6 1.0 C4 C:DC0 4.6 54.0 1.0 N3 C:DC0 4.6 55.7 1.0 N4 C:DC0 4.8 54.6 1.0 CA A:HIS70 4.8 52.3 1.0

Zinc binding site 2 out of 2 in the Structure of APOBEC3A (E72A Inactive Mutant) in Complex with Ttc- Hairpin Dna Substrate


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Structure of APOBEC3A (E72A Inactive Mutant) in Complex with Ttc- Hairpin Dna Substrate within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn202 b:65.6 occ:0.50 ND1 B:HIS70 2.0 55.7 1.0 SG B:CYS106 2.0 100.1 1.0 CL B:CL203 2.3 61.7 1.0 SG B:CYS101 2.7 77.8 1.0 CG B:HIS70 2.8 50.2 1.0 CB B:HIS70 3.0 49.6 1.0 CB B:CYS106 3.0 87.0 1.0 CE1 B:HIS70 3.0 55.8 1.0 CB B:CYS101 3.4 65.6 1.0 CD2 B:HIS70 3.9 52.5 1.0 N B:CYS101 3.9 60.4 1.0 NE2 B:HIS70 4.0 53.2 1.0 CA B:CYS101 4.1 65.4 1.0 O H:HOH101 4.3 42.5 1.0 CB B:TRP104 4.4 107.7 1.0 N3 H:DC0 4.4 52.0 1.0 C4 H:DC0 4.4 51.6 1.0 CA B:CYS106 4.4 83.7 1.0 CA B:HIS70 4.6 51.1 1.0 N4 H:DC0 4.6 52.3 1.0 C B:CYS101 4.6 66.3 1.0 O B:CYS101 4.6 64.3 1.0 N B:CYS106 4.7 89.1 1.0 C2 H:DC0 4.9 52.2 1.0 C5 H:DC0 4.9 51.4 1.0

S.Harjes, H.M.Kurup, V.Filichev, E.Harjes, G.B.Jameson. Dna-Based Inhibitors Restrict Mutagenic Activity of APOBEC3 in Cells To Be Published.