Zinc in PDB 8fik: APOBEC3A E72A Inactive Mutant in Complex with Attc-Hairpin Dna Substrate

All present enzymatic activity of APOBEC3A E72A Inactive Mutant in Complex with Attc-Hairpin Dna Substrate:
3.5.4.38;

The structure of APOBEC3A E72A Inactive Mutant in Complex with Attc-Hairpin Dna Substrate, PDB code: 8fik was solved by S.Harjes, G.B.Jameson, E.Harjes, V.V.Filichev, H.M.Kurup, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	48.17 / 1.91
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	55.001, 57.229, 92.752, 90, 106.48, 90
R / Rfree (%)	20 / 23.2

The structure of APOBEC3A E72A Inactive Mutant in Complex with Attc-Hairpin Dna Substrate also contains other interesting chemical elements:

Chlorine

(Cl)

2 atoms

The binding sites of Zinc atom in the APOBEC3A E72A Inactive Mutant in Complex with Attc-Hairpin Dna Substrate (pdb code 8fik). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the APOBEC3A E72A Inactive Mutant in Complex with Attc-Hairpin Dna Substrate, PDB code: 8fik:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the APOBEC3A E72A Inactive Mutant in Complex with Attc-Hairpin Dna Substrate


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of APOBEC3A E72A Inactive Mutant in Complex with Attc-Hairpin Dna Substrate within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn201 b:31.2 occ:1.00 ND1 A:HIS70 2.0 29.6 1.0 SG A:CYS106 2.2 35.6 1.0 CL A:CL202 2.3 30.8 1.0 SG A:CYS101 2.3 35.3 1.0 CB A:CYS106 2.9 37.0 1.0 CG A:HIS70 3.0 28.3 1.0 CE1 A:HIS70 3.0 30.9 1.0 CB A:HIS70 3.3 26.0 1.0 CB A:CYS101 3.4 31.0 1.0 N A:CYS101 4.0 28.1 1.0 NE2 A:HIS70 4.1 32.3 1.0 CD2 A:HIS70 4.1 30.7 1.0 CA A:CYS101 4.2 29.6 1.0 CA A:CYS106 4.3 34.3 1.0 CB A:TRP104 4.3 41.7 1.0 O A:CYS101 4.4 30.1 1.0 O A:HOH338 4.5 32.4 1.0 C A:CYS101 4.5 31.3 1.0 N A:CYS106 4.5 33.6 1.0 C4 D:DC0 4.8 25.8 1.0 N3 D:DC0 4.8 25.5 1.0 CA A:HIS70 4.8 24.3 1.0 O D:HOH104 4.8 22.7 1.0 N4 D:DC0 4.9 26.7 1.0

Zinc binding site 2 out of 2 in the APOBEC3A E72A Inactive Mutant in Complex with Attc-Hairpin Dna Substrate


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of APOBEC3A E72A Inactive Mutant in Complex with Attc-Hairpin Dna Substrate within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn201 b:28.6 occ:1.00 ND1 B:HIS70 2.1 26.0 1.0 SG B:CYS106 2.2 33.2 1.0 SG B:CYS101 2.3 33.5 1.0 CL B:CL202 2.3 33.5 1.0 CB B:CYS106 2.9 34.6 1.0 CG B:HIS70 3.0 24.6 1.0 CE1 B:HIS70 3.0 27.1 1.0 CB B:HIS70 3.3 22.3 1.0 CB B:CYS101 3.5 27.2 1.0 N B:CYS101 4.0 23.4 1.0 CB B:TRP104 4.1 39.4 1.0 NE2 B:HIS70 4.1 26.6 1.0 CD2 B:HIS70 4.1 26.4 1.0 CA B:CYS101 4.2 26.8 1.0 CA B:CYS106 4.3 32.4 1.0 N B:CYS106 4.5 32.5 1.0 O B:CYS101 4.5 24.6 1.0 O B:HOH351 4.5 35.3 1.0 C B:CYS101 4.6 26.6 1.0 O H:HOH107 4.7 22.4 1.0 N3 H:DC0 4.8 24.0 1.0 CA B:HIS70 4.8 21.6 1.0 C4 H:DC0 4.8 24.9 1.0

S.Harjes, H.M.Kurup, V.Filichev, E.Harjes, G.B.Jameson. Dna-Based Inhibitors Restrict Mutagenic Activity of APOBEC3 in Cells To Be Published.