Zinc in PDB 8e0f: Human Adenosine Deaminase Acting on Dsrna (ADAR2-Rd) Bound to Dsrna Containing A G-G Pair Adjacent to the Target Site

All present enzymatic activity of Human Adenosine Deaminase Acting on Dsrna (ADAR2-Rd) Bound to Dsrna Containing A G-G Pair Adjacent to the Target Site:
3.5.4.37;

The structure of Human Adenosine Deaminase Acting on Dsrna (ADAR2-Rd) Bound to Dsrna Containing A G-G Pair Adjacent to the Target Site, PDB code: 8e0f was solved by X.E.Wilcox, A.J.Fisher, P.A.Beal, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	65.73 / 2.70
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	171.518, 63.389, 142.135, 90, 117.69, 90
R / Rfree (%)	19.3 / 23.1

The binding sites of Zinc atom in the Human Adenosine Deaminase Acting on Dsrna (ADAR2-Rd) Bound to Dsrna Containing A G-G Pair Adjacent to the Target Site (pdb code 8e0f). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Human Adenosine Deaminase Acting on Dsrna (ADAR2-Rd) Bound to Dsrna Containing A G-G Pair Adjacent to the Target Site, PDB code: 8e0f:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Human Adenosine Deaminase Acting on Dsrna (ADAR2-Rd) Bound to Dsrna Containing A G-G Pair Adjacent to the Target Site


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Human Adenosine Deaminase Acting on Dsrna (ADAR2-Rd) Bound to Dsrna Containing A G-G Pair Adjacent to the Target Site within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn802 b:55.0 occ:1.00 O6 C:8AZ13 2.0 60.6 1.0 ND1 A:HIS394 2.0 56.2 1.0 SG A:CYS451 2.3 60.7 1.0 SG A:CYS516 2.4 52.5 1.0 CE1 A:HIS394 2.9 59.1 1.0 CG A:HIS394 3.1 60.6 1.0 C6 C:8AZ13 3.1 66.7 1.0 CB A:CYS516 3.2 55.5 1.0 CB A:CYS451 3.3 58.1 1.0 NZ A:LYS483 3.5 54.0 1.0 CB A:HIS394 3.5 56.7 1.0 C5 C:8AZ13 3.5 63.6 1.0 N1 C:8AZ13 3.6 64.4 1.0 N A:CYS451 3.8 53.1 1.0 OE2 A:GLU396 3.8 60.1 1.0 NE2 A:HIS394 4.0 54.5 1.0 CE A:LYS483 4.1 58.2 1.0 N A:CYS516 4.1 52.0 1.0 C4 C:8AZ13 4.1 62.0 1.0 C2 C:8AZ13 4.1 63.5 1.0 CA A:CYS451 4.1 53.0 1.0 N7 C:8AZ13 4.1 61.6 1.0 CD2 A:HIS394 4.1 59.2 1.0 CA A:CYS516 4.3 53.3 1.0 CD A:GLU396 4.3 60.0 1.0 N3 C:8AZ13 4.4 60.9 1.0 OE1 A:GLU396 4.5 59.6 1.0 N9 C:8AZ13 4.8 60.9 1.0 N8 C:8AZ13 4.9 68.3 1.0 C A:PRO450 4.9 60.4 1.0 CA A:HIS394 5.0 55.5 1.0 C A:CYS451 5.0 52.8 1.0

Zinc binding site 2 out of 2 in the Human Adenosine Deaminase Acting on Dsrna (ADAR2-Rd) Bound to Dsrna Containing A G-G Pair Adjacent to the Target Site


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Human Adenosine Deaminase Acting on Dsrna (ADAR2-Rd) Bound to Dsrna Containing A G-G Pair Adjacent to the Target Site within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn802 b:62.5 occ:1.00 O B:HOH903 2.0 53.6 1.0 ND1 B:HIS394 2.1 66.5 1.0 SG B:CYS451 2.3 58.0 1.0 SG B:CYS516 2.3 65.2 1.0 CE1 B:HIS394 2.9 62.5 1.0 CB B:CYS516 3.0 61.0 1.0 CG B:HIS394 3.1 59.1 1.0 CB B:CYS451 3.2 60.1 1.0 CE B:LYS483 3.5 60.8 1.0 CB B:HIS394 3.6 58.5 1.0 N B:CYS516 3.8 61.4 1.0 N B:CYS451 3.8 61.5 1.0 OE2 B:GLU396 3.9 55.2 1.0 CA B:CYS516 4.0 58.8 1.0 NE2 B:HIS394 4.0 61.5 1.0 CA B:CYS451 4.1 57.9 1.0 NZ B:LYS483 4.1 59.9 1.0 CD2 B:HIS394 4.2 59.8 1.0 CD B:GLU396 4.3 57.8 1.0 OE1 B:GLU396 4.6 60.9 1.0 O B:CYS451 4.6 67.6 1.0 CD B:LYS483 4.7 61.3 1.0 C B:CYS451 4.7 63.3 1.0

E.E.Doherty, A.Karki, X.E.Wilcox, H.G.Mendoza, A.Manjunath, V.J.Matos, A.J.Fisher, P.A.Beal. Adar Activation By Inducing A Syn Conformation at Guanosine Adjacent to An Editing Site. Nucleic Acids Res. V. 50 10857 2022.
ISSN: ESSN 1362-4962
PubMed: 36243986
DOI: 10.1093/NAR/GKAC897