Zinc in PDB 8dzj: Cryo-Em Structure of Acidibacillus Sulfuroxidans CAS12F in Complex with Sgrna and Target Dna

The binding sites of Zinc atom in the Cryo-Em Structure of Acidibacillus Sulfuroxidans CAS12F in Complex with Sgrna and Target Dna (pdb code 8dzj). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total only one binding site of Zinc was determined in the Cryo-Em Structure of Acidibacillus Sulfuroxidans CAS12F in Complex with Sgrna and Target Dna, PDB code: 8dzj:

Zinc binding site 1 out of 1 in the Cryo-Em Structure of Acidibacillus Sulfuroxidans CAS12F in Complex with Sgrna and Target Dna


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Cryo-Em Structure of Acidibacillus Sulfuroxidans CAS12F in Complex with Sgrna and Target Dna within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn501 b:57.0 occ:1.00 SG A:CYS391 2.3 37.3 1.0 SG A:CYS375 2.3 35.0 1.0 SG A:CYS394 2.3 37.1 1.0 SG A:CYS372 2.8 30.1 1.0 CB A:CYS391 3.0 37.3 1.0 CB A:CYS375 3.2 35.0 1.0 CB A:CYS394 3.6 37.1 1.0 CB A:CYS372 3.6 30.1 1.0 N A:CYS394 3.8 37.1 1.0 N A:CYS375 3.9 35.0 1.0 CB A:TYR396 4.0 36.2 1.0 CA A:CYS375 4.2 35.0 1.0 CA A:CYS394 4.2 37.1 1.0 CA A:CYS391 4.4 37.3 1.0 C A:CYS391 4.6 37.3 1.0 C A:ALA393 4.6 35.2 1.0 N A:TYR396 4.6 36.2 1.0 C A:CYS394 4.7 37.1 1.0 O A:CYS391 4.7 37.3 1.0 CB A:ALA393 4.7 35.2 1.0 CB A:GLU374 4.7 34.5 1.0 N A:GLY395 4.8 33.5 1.0 N A:ALA393 4.9 35.2 1.0 CG A:TYR396 4.9 36.2 1.0 CA A:TYR396 5.0 36.2 1.0 CA A:ALA393 5.0 35.2 1.0 CD2 A:TYR396 5.0 36.2 1.0 C A:CYS375 5.0 35.0 1.0 C A:GLU374 5.0 34.5 1.0

T.Wu, C.Liu, S.Zou, R.Lyu, B.Yang, H.Yan, M.Zhao, W.Tang. An Engineered Hypercompact Crispr-CAS12F System with Boosted Gene-Editing Activity. Nat.Chem.Biol. 2023.
ISSN: ESSN 1552-4469
PubMed: 37400536
DOI: 10.1038/S41589-023-01380-9