Zinc in PDB 8d1v: Cryo-Em Structure of Guide Rna and Target Rna Bound CAS7-11
Zinc Binding Sites:
The binding sites of Zinc atom in the Cryo-Em Structure of Guide Rna and Target Rna Bound CAS7-11
(pdb code 8d1v). This binding sites where shown within
5.0 Angstroms radius around Zinc atom.
In total 4 binding sites of Zinc where determined in the
Cryo-Em Structure of Guide Rna and Target Rna Bound CAS7-11, PDB code: 8d1v:
Jump to Zinc binding site number:
1;
2;
3;
4;
Zinc binding site 1 out
of 4 in 8d1v
Go back to
Zinc Binding Sites List in 8d1v
Zinc binding site 1 out
of 4 in the Cryo-Em Structure of Guide Rna and Target Rna Bound CAS7-11
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 1 of Cryo-Em Structure of Guide Rna and Target Rna Bound CAS7-11 within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Zn1701
b:69.8
occ:1.00
|
SG
|
A:CYS1312
|
2.3
|
42.3
|
1.0
|
SG
|
A:CYS1345
|
2.3
|
42.8
|
1.0
|
SG
|
A:CYS965
|
2.3
|
16.2
|
1.0
|
SG
|
A:CYS1342
|
2.3
|
16.5
|
1.0
|
CB
|
A:CYS1342
|
3.2
|
24.2
|
1.0
|
CB
|
A:CYS1345
|
3.3
|
13.5
|
1.0
|
CB
|
A:CYS1312
|
3.3
|
39.3
|
1.0
|
CB
|
A:CYS965
|
3.5
|
17.0
|
1.0
|
N
|
A:CYS1312
|
3.8
|
41.8
|
1.0
|
N
|
A:CYS1342
|
3.8
|
26.0
|
1.0
|
CA
|
A:CYS1342
|
4.1
|
20.9
|
1.0
|
N
|
A:CYS965
|
4.1
|
14.6
|
1.0
|
CA
|
A:CYS1312
|
4.2
|
39.7
|
1.0
|
N
|
A:CYS1345
|
4.2
|
12.8
|
1.0
|
CA
|
A:CYS1345
|
4.3
|
10.7
|
1.0
|
CA
|
A:CYS965
|
4.3
|
17.4
|
1.0
|
CG
|
A:ARG967
|
4.5
|
10.2
|
1.0
|
NH2
|
A:ARG876
|
4.6
|
37.1
|
1.0
|
C
|
A:CYS1342
|
4.8
|
22.6
|
1.0
|
NE
|
A:ARG876
|
4.8
|
37.8
|
1.0
|
C
|
A:PRO1311
|
4.8
|
37.5
|
1.0
|
O
|
A:CYS1342
|
4.9
|
24.7
|
1.0
|
O
|
A:CYS965
|
4.9
|
21.4
|
1.0
|
C
|
A:CYS965
|
4.9
|
21.2
|
1.0
|
CZ
|
A:ARG876
|
4.9
|
38.7
|
1.0
|
CA
|
A:PRO1311
|
5.0
|
35.7
|
1.0
|
C
|
A:LEU1341
|
5.0
|
32.7
|
1.0
|
|
Zinc binding site 2 out
of 4 in 8d1v
Go back to
Zinc Binding Sites List in 8d1v
Zinc binding site 2 out
of 4 in the Cryo-Em Structure of Guide Rna and Target Rna Bound CAS7-11
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 2 of Cryo-Em Structure of Guide Rna and Target Rna Bound CAS7-11 within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Zn1702
b:78.0
occ:1.00
|
CB
|
A:CYS708
|
1.8
|
42.8
|
1.0
|
ND1
|
A:HIS703
|
2.1
|
48.7
|
1.0
|
SG
|
A:CYS706
|
2.3
|
64.5
|
1.0
|
SG
|
A:CYS711
|
2.3
|
40.1
|
1.0
|
SG
|
A:CYS708
|
2.3
|
47.8
|
1.0
|
CE1
|
A:HIS703
|
2.4
|
49.8
|
1.0
|
CA
|
A:CYS708
|
3.2
|
42.0
|
1.0
|
CG
|
A:HIS703
|
3.4
|
48.0
|
1.0
|
CB
|
A:CYS706
|
3.5
|
58.0
|
1.0
|
CB
|
A:CYS711
|
3.7
|
31.4
|
1.0
|
NE2
|
A:HIS703
|
3.7
|
49.0
|
1.0
|
N
|
A:CYS708
|
3.8
|
47.1
|
1.0
|
CB
|
A:HIS703
|
4.2
|
48.6
|
1.0
|
CD2
|
A:HIS703
|
4.2
|
47.3
|
1.0
|
N
|
A:CYS711
|
4.2
|
33.9
|
1.0
|
C
|
A:CYS708
|
4.3
|
39.6
|
1.0
|
CA
|
A:HIS703
|
4.6
|
52.4
|
1.0
|
CA
|
A:CYS711
|
4.6
|
28.8
|
1.0
|
OE1
|
A:GLU693
|
4.6
|
59.9
|
1.0
|
C
|
A:GLU707
|
4.7
|
56.1
|
1.0
|
CA
|
A:CYS706
|
4.8
|
58.8
|
1.0
|
C
|
A:CYS706
|
4.8
|
57.1
|
1.0
|
O
|
A:CYS708
|
4.8
|
42.0
|
1.0
|
N
|
A:LEU709
|
5.0
|
34.2
|
1.0
|
|
Zinc binding site 3 out
of 4 in 8d1v
Go back to
Zinc Binding Sites List in 8d1v
Zinc binding site 3 out
of 4 in the Cryo-Em Structure of Guide Rna and Target Rna Bound CAS7-11
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 3 of Cryo-Em Structure of Guide Rna and Target Rna Bound CAS7-11 within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Zn1703
b:80.5
occ:1.00
|
SG
|
A:CYS477
|
2.3
|
37.7
|
1.0
|
SG
|
A:CYS463
|
2.3
|
49.2
|
1.0
|
SG
|
A:CYS474
|
2.3
|
31.9
|
1.0
|
SG
|
A:CYS472
|
2.3
|
43.9
|
1.0
|
CB
|
A:CYS472
|
3.1
|
34.8
|
1.0
|
CB
|
A:CYS477
|
3.3
|
25.6
|
1.0
|
CB
|
A:CYS474
|
3.4
|
29.4
|
1.0
|
CB
|
A:CYS463
|
3.5
|
36.3
|
1.0
|
N
|
A:CYS477
|
4.1
|
22.1
|
1.0
|
N
|
A:CYS474
|
4.2
|
30.9
|
1.0
|
CA
|
A:CYS477
|
4.3
|
19.3
|
1.0
|
CB
|
A:ALA465
|
4.3
|
44.9
|
1.0
|
CA
|
A:CYS474
|
4.3
|
29.7
|
1.0
|
CA
|
A:CYS472
|
4.4
|
37.5
|
1.0
|
CA
|
A:CYS463
|
4.5
|
37.3
|
1.0
|
C
|
A:CYS472
|
4.5
|
35.0
|
1.0
|
O
|
A:CYS472
|
4.7
|
38.9
|
1.0
|
C
|
A:CYS474
|
4.9
|
29.8
|
1.0
|
N
|
A:MET473
|
4.9
|
33.2
|
1.0
|
O
|
A:CYS474
|
5.0
|
33.3
|
1.0
|
|
Zinc binding site 4 out
of 4 in 8d1v
Go back to
Zinc Binding Sites List in 8d1v
Zinc binding site 4 out
of 4 in the Cryo-Em Structure of Guide Rna and Target Rna Bound CAS7-11
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 4 of Cryo-Em Structure of Guide Rna and Target Rna Bound CAS7-11 within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Zn1704
b:93.5
occ:1.00
|
SG
|
A:CYS86
|
2.3
|
54.1
|
1.0
|
SG
|
A:CYS126
|
2.3
|
54.3
|
1.0
|
SG
|
A:CYS123
|
2.3
|
59.6
|
1.0
|
SG
|
A:CYS115
|
2.3
|
59.0
|
1.0
|
CB
|
A:CYS115
|
3.3
|
54.8
|
1.0
|
CB
|
A:CYS123
|
3.4
|
52.9
|
1.0
|
CB
|
A:CYS126
|
3.5
|
48.3
|
1.0
|
CB
|
A:CYS86
|
3.6
|
51.3
|
1.0
|
N
|
A:CYS115
|
3.8
|
54.9
|
1.0
|
N
|
A:CYS123
|
3.9
|
56.0
|
1.0
|
N
|
A:CYS86
|
4.1
|
51.7
|
1.0
|
CA
|
A:CYS115
|
4.2
|
54.6
|
1.0
|
CA
|
A:CYS123
|
4.2
|
55.1
|
1.0
|
N
|
A:CYS126
|
4.2
|
48.0
|
1.0
|
CA
|
A:CYS86
|
4.4
|
51.0
|
1.0
|
NH1
|
A:ARG131
|
4.4
|
53.6
|
1.0
|
CA
|
A:CYS126
|
4.5
|
49.0
|
1.0
|
O
|
A:CYS123
|
4.7
|
55.6
|
1.0
|
C
|
A:CYS123
|
4.7
|
55.9
|
1.0
|
O
|
A:CYS86
|
4.8
|
53.6
|
1.0
|
C
|
A:CYS86
|
4.8
|
51.1
|
1.0
|
C
|
A:PRO114
|
4.9
|
52.5
|
1.0
|
|
Reference:
H.N.Goswami,
J.Rai,
A.Das,
H.Li.
Molecular Mechanism of Active CAS7-11 in Processing Crispr Rna and Interfering Target Rna. Elife V. 11 2022.
ISSN: ESSN 2050-084X
PubMed: 36190192
DOI: 10.7554/ELIFE.81678
Page generated: Wed Oct 30 19:00:57 2024
|