Zinc in PDB 8c0g: Sars-Cov NSP16-NSP10 Complexed with N7-Gtp

The structure of Sars-Cov NSP16-NSP10 Complexed with N7-Gtp, PDB code: 8c0g was solved by F.Ferron, C.Debarnot, B.Coutard, B.Canard, Marseilles Structural Genomicsprogram @ Afmb (Msgp), with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	92.52 / 1.88
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	67.232, 185.042, 128.577, 90, 90, 90
R / Rfree (%)	24.5 / 26.3

The structure of Sars-Cov NSP16-NSP10 Complexed with N7-Gtp also contains other interesting chemical elements:

Sodium

(Na)

1 atom

The binding sites of Zinc atom in the Sars-Cov NSP16-NSP10 Complexed with N7-Gtp (pdb code 8c0g). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Sars-Cov NSP16-NSP10 Complexed with N7-Gtp, PDB code: 8c0g:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Sars-Cov NSP16-NSP10 Complexed with N7-Gtp


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Sars-Cov NSP16-NSP10 Complexed with N7-Gtp within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn401 b:49.6 occ:1.00 NE2 B:HIS83 2.0 56.2 1.0 SG B:CYS77 2.1 46.5 1.0 SG B:CYS74 2.2 46.3 1.0 SG B:CYS90 2.4 55.6 1.0 CD2 B:HIS83 2.9 55.8 1.0 CE1 B:HIS83 3.1 56.7 1.0 CB B:CYS74 3.2 42.4 1.0 CB B:CYS90 3.3 58.0 1.0 CB B:CYS77 3.4 42.1 1.0 N B:CYS77 3.7 41.6 1.0 CA B:CYS77 4.0 41.4 1.0 CG B:HIS83 4.1 56.0 1.0 ND1 B:HIS83 4.2 56.8 1.0 CA B:CYS90 4.6 59.5 1.0 C B:TYR76 4.6 42.1 1.0 O B:HOH560 4.6 71.0 1.0 CA B:CYS74 4.6 41.2 1.0 CB B:TYR76 4.6 42.9 1.0 CB B:LYS93 4.8 51.0 1.0 CA B:TYR76 4.9 41.8 1.0 N B:LYS93 5.0 48.5 1.0

Zinc binding site 2 out of 2 in the Sars-Cov NSP16-NSP10 Complexed with N7-Gtp


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Sars-Cov NSP16-NSP10 Complexed with N7-Gtp within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn402 b:85.2 occ:1.00 SG B:CYS128 2.2 92.3 1.0 SG B:CYS117 2.2 76.6 1.0 SG B:CYS120 2.5 81.3 1.0 SG B:CYS130 2.7 111.5 1.0 CB B:CYS117 3.0 73.0 1.0 CB B:CYS130 3.3 108.5 1.0 CB B:CYS120 3.5 78.1 1.0 CB B:CYS128 3.5 92.4 1.0 N B:CYS120 3.9 78.2 1.0 CG2 B:VAL119 4.0 82.0 1.0 N B:CYS130 4.1 105.5 1.0 CA B:CYS128 4.2 92.7 1.0 CA B:CYS120 4.3 77.4 1.0 CA B:CYS130 4.3 107.8 1.0 CA B:CYS117 4.5 71.6 1.0 C B:CYS128 4.7 95.6 1.0 N B:SER129 4.7 98.0 1.0 C B:VAL119 5.0 79.4 1.0

F.Ferron, B.Canard, C.Debarnot, B.Coutard. Observation of N7-Gtp in NSP16-NSP10 Sars-Cov To Be Published.