Zinc in PDB 8adh: Interdomain Motion in Liver Alcohol Dehydrogenase. Structural and Energetic Analysis of the Hinge Bending Mode

All present enzymatic activity of Interdomain Motion in Liver Alcohol Dehydrogenase. Structural and Energetic Analysis of the Hinge Bending Mode:
1.1.1.1;

The structure of Interdomain Motion in Liver Alcohol Dehydrogenase. Structural and Energetic Analysis of the Hinge Bending Mode, PDB code: 8adh was solved by T.A.Jones, H.Eklund, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	N/A / 2.40
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	56.000, 75.200, 181.700, 90.00, 90.00, 90.00
R / Rfree (%)	n/a / n/a

The binding sites of Zinc atom in the Interdomain Motion in Liver Alcohol Dehydrogenase. Structural and Energetic Analysis of the Hinge Bending Mode (pdb code 8adh). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Interdomain Motion in Liver Alcohol Dehydrogenase. Structural and Energetic Analysis of the Hinge Bending Mode, PDB code: 8adh:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Interdomain Motion in Liver Alcohol Dehydrogenase. Structural and Energetic Analysis of the Hinge Bending Mode


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Interdomain Motion in Liver Alcohol Dehydrogenase. Structural and Energetic Analysis of the Hinge Bending Mode within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn376 b:19.6 occ:1.00 O A:HOH417 2.0 17.4 1.0 SG A:CYS174 2.1 18.2 1.0 SG A:CYS46 2.2 15.4 1.0 NE2 A:HIS67 2.3 13.7 1.0 CE1 A:HIS67 3.2 13.1 1.0 CD2 A:HIS67 3.3 13.4 1.0 CB A:CYS174 3.3 18.1 1.0 CB A:CYS46 3.3 16.6 1.0 O A:HOH429 3.9 35.7 1.0 OG A:SER48 4.1 16.7 1.0 O A:HOH401 4.2 15.1 1.0 O A:HOH402 4.3 26.4 1.0 ND1 A:HIS67 4.3 13.5 1.0 CG A:HIS67 4.4 12.9 1.0 CB A:SER48 4.5 17.0 1.0 CA A:CYS174 4.6 18.1 1.0 CA A:CYS46 4.7 17.1 1.0 CE1 A:PHE93 4.8 12.3 1.0 N A:GLY175 4.9 18.2 1.0 CZ A:PHE93 5.0 11.9 1.0 N A:SER48 5.0 18.3 1.0

Zinc binding site 2 out of 2 in the Interdomain Motion in Liver Alcohol Dehydrogenase. Structural and Energetic Analysis of the Hinge Bending Mode


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Interdomain Motion in Liver Alcohol Dehydrogenase. Structural and Energetic Analysis of the Hinge Bending Mode within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn377 b:23.7 occ:1.00 SG A:CYS111 2.2 21.3 1.0 SG A:CYS103 2.3 19.5 1.0 SG A:CYS97 2.4 22.6 1.0 SG A:CYS100 2.4 22.1 1.0 CB A:CYS103 3.3 20.8 1.0 N A:CYS97 3.3 19.4 1.0 CB A:CYS97 3.3 21.0 1.0 CB A:CYS111 3.5 22.4 1.0 CB A:CYS100 3.6 21.9 1.0 CA A:CYS97 3.7 20.7 1.0 N A:GLY98 3.8 22.0 1.0 N A:CYS100 3.8 22.6 1.0 CA A:CYS111 3.9 22.7 1.0 N A:LEU112 4.1 24.4 1.0 C A:CYS97 4.2 21.4 1.0 N A:CYS103 4.2 21.7 1.0 CA A:CYS100 4.2 22.3 1.0 C A:GLN96 4.3 18.6 1.0 N A:LYS99 4.3 23.0 1.0 CA A:CYS103 4.3 21.3 1.0 C A:CYS111 4.5 23.6 1.0 CA A:GLY98 4.7 22.2 1.0 CA A:GLN96 4.8 18.0 1.0 C A:LYS99 4.8 23.1 1.0 C A:GLY98 4.9 22.7 1.0 N A:LYS113 4.9 25.4 1.0

F.Colonna-Cesari, D.Perahia, M.Karplus, H.Eklund, C.I.Braden, O.Tapia. Interdomain Motion in Liver Alcohol Dehydrogenase. Structural and Energetic Analysis of the Hinge Bending Mode. J.Biol.Chem. V. 261 15273 1986.
ISSN: ISSN 0021-9258
PubMed: 3771574