Zinc in PDB 8a9k: Cryo-Em Structure of USP1-UAF1 Bound to Fanci and Mono-Ubiquitinated FANCD2 with ML323 (Consensus Reconstruction)

All present enzymatic activity of Cryo-Em Structure of USP1-UAF1 Bound to Fanci and Mono-Ubiquitinated FANCD2 with ML323 (Consensus Reconstruction):
3.4.19.12;

The binding sites of Zinc atom in the Cryo-Em Structure of USP1-UAF1 Bound to Fanci and Mono-Ubiquitinated FANCD2 with ML323 (Consensus Reconstruction) (pdb code 8a9k). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total only one binding site of Zinc was determined in the Cryo-Em Structure of USP1-UAF1 Bound to Fanci and Mono-Ubiquitinated FANCD2 with ML323 (Consensus Reconstruction), PDB code: 8a9k:

Zinc binding site 1 out of 1 in the Cryo-Em Structure of USP1-UAF1 Bound to Fanci and Mono-Ubiquitinated FANCD2 with ML323 (Consensus Reconstruction)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Cryo-Em Structure of USP1-UAF1 Bound to Fanci and Mono-Ubiquitinated FANCD2 with ML323 (Consensus Reconstruction) within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn1000 b:25.8 occ:1.00 SG D:CYS443 2.3 19.1 1.0 SG D:CYS509 2.3 19.1 1.0 SG D:CYS506 2.3 19.1 1.0 SG D:CYS440 2.4 19.1 1.0 CB D:CYS506 3.1 19.1 1.0 CB D:CYS440 3.1 19.1 1.0 CB D:CYS443 3.3 19.1 1.0 CB D:CYS509 3.6 19.1 1.0 N D:CYS443 3.6 19.1 1.0 CA D:CYS443 4.1 19.1 1.0 N D:CYS509 4.1 19.1 1.0 CB D:GLU442 4.4 19.1 1.0 OG1 D:THR513 4.4 19.1 1.0 CA D:CYS509 4.5 19.1 1.0 CG2 D:THR513 4.5 19.1 1.0 CA D:CYS506 4.6 19.1 1.0 CA D:CYS440 4.6 19.1 1.0 CB D:ASN508 4.6 19.1 1.0 OD1 D:ASN508 4.7 19.1 1.0 C D:GLU442 4.7 19.1 1.0 N D:GLU442 4.9 19.1 1.0 CA D:GLU442 4.9 19.1 1.0 C D:CYS443 4.9 19.1 1.0 C D:ASN508 5.0 19.1 1.0 N D:GLU444 5.0 19.1 1.0

M.L.Rennie, C.Arkinson, V.K.Chaugule, H.Walden. Cryo-Em Reveals A Mechanism of USP1 Inhibition Through A Cryptic Binding Site. Sci Adv V. 8 Q6353 2022.
ISSN: ESSN 2375-2548
PubMed: 36170365
DOI: 10.1126/SCIADV.ABQ6353