Zinc in PDB 7zju: Structure of Human USPL1 in Covalent Complex with SUMO3-2BR Probe

The structure of Structure of Human USPL1 in Covalent Complex with SUMO3-2BR Probe, PDB code: 7zju was solved by Z.Zhao, M.Gersch, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	54.47 / 2.17
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	66.06, 84.833, 71.106, 90, 92.26, 90
R / Rfree (%)	19.2 / 23.8

The structure of Structure of Human USPL1 in Covalent Complex with SUMO3-2BR Probe also contains other interesting chemical elements:

Chlorine	(Cl)	2 atoms
Calcium	(Ca)	2 atoms

The binding sites of Zinc atom in the Structure of Human USPL1 in Covalent Complex with SUMO3-2BR Probe (pdb code 7zju). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Structure of Human USPL1 in Covalent Complex with SUMO3-2BR Probe, PDB code: 7zju:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Structure of Human USPL1 in Covalent Complex with SUMO3-2BR Probe


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Structure of Human USPL1 in Covalent Complex with SUMO3-2BR Probe within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn801 b:50.6 occ:1.00 SG A:CYS361 2.3 48.3 1.0 SG A:CYS400 2.3 47.5 1.0 SG A:CYS364 2.3 59.7 1.0 SG A:CYS397 2.4 49.5 1.0 CB A:CYS397 3.2 48.5 1.0 CB A:CYS361 3.2 46.3 1.0 CB A:CYS400 3.4 50.6 1.0 CB A:CYS364 3.5 58.0 1.0 N A:CYS364 3.6 56.4 1.0 N A:CYS400 3.8 56.4 1.0 CA A:CYS364 4.1 52.5 1.0 CA A:CYS400 4.2 60.9 1.0 CB A:GLN363 4.4 60.9 1.0 CB A:ASN399 4.4 54.5 1.0 CB A:HIS366 4.5 46.0 1.0 C A:CYS364 4.6 55.6 1.0 CA A:CYS397 4.6 45.9 1.0 C A:ASN399 4.7 51.8 1.0 C A:GLN363 4.7 53.8 1.0 CA A:CYS361 4.7 45.2 1.0 O A:CYS364 4.7 64.2 1.0 CB A:SER402 4.8 48.5 1.0 C A:CYS400 4.9 54.8 1.0 CA A:GLN363 4.9 55.9 1.0 CA A:ASN399 4.9 51.2 1.0 N A:ASN399 4.9 53.8 1.0 N A:GLN363 4.9 56.6 1.0

Zinc binding site 2 out of 2 in the Structure of Human USPL1 in Covalent Complex with SUMO3-2BR Probe


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Structure of Human USPL1 in Covalent Complex with SUMO3-2BR Probe within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn801 b:51.7 occ:1.00 SG C:CYS397 2.3 49.9 1.0 SG C:CYS400 2.3 54.6 1.0 SG C:CYS364 2.3 54.5 1.0 SG C:CYS361 2.4 50.6 1.0 CB C:CYS361 3.1 51.1 1.0 CB C:CYS397 3.1 45.7 1.0 CB C:CYS400 3.3 60.7 1.0 CB C:CYS364 3.5 54.2 1.0 N C:CYS364 3.8 57.9 1.0 N C:CYS400 3.9 62.1 1.0 CA C:CYS400 4.1 66.5 1.0 CA C:CYS364 4.1 59.4 1.0 CB C:HIS366 4.3 53.2 1.0 CA C:CYS397 4.6 42.7 1.0 CA C:CYS361 4.6 51.6 1.0 C C:CYS364 4.6 61.9 1.0 C C:ASN399 4.6 64.7 1.0 C C:CYS400 4.7 60.6 1.0 CB C:SER402 4.7 67.8 1.0 O C:CYS400 4.8 69.2 1.0 CB C:ASN399 4.9 59.1 1.0 CB C:GLN363 4.9 68.3 1.0 O C:CYS364 4.9 64.3 1.0 C C:GLN363 4.9 59.0 1.0 N C:HIS366 4.9 53.2 1.0 N C:ASN399 4.9 53.5 1.0

Z.Zhao, R.O’Dea, K.Wendrich, N.Kazi, M.Gersch. Native Semisynthesis of Isopeptide-Linked Substrates For Specificity Analysis of Deubiquitinases and Ubl Proteases J.Am.Chem.Soc. 2023.
ISSN: ESSN 1520-5126
DOI: 10.1021/JACS.3C04062