Zinc in PDB 7yrs: Crystal Structure of Lactobacillus Rhamnosus 4-Deoxy-L-Threo-5- Hexosulose-Uronate Ketol-Isomerase Kdui Complexed with Mops

Enzymatic activity of Crystal Structure of Lactobacillus Rhamnosus 4-Deoxy-L-Threo-5- Hexosulose-Uronate Ketol-Isomerase Kdui Complexed with Mops

All present enzymatic activity of Crystal Structure of Lactobacillus Rhamnosus 4-Deoxy-L-Threo-5- Hexosulose-Uronate Ketol-Isomerase Kdui Complexed with Mops:
5.3.1.17;

Protein crystallography data

The structure of Crystal Structure of Lactobacillus Rhamnosus 4-Deoxy-L-Threo-5- Hexosulose-Uronate Ketol-Isomerase Kdui Complexed with Mops, PDB code: 7yrs was solved by Y.Yamamoto, S.Oiki, R.Takase, B.Mikami, W.Hashimoto, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 49.55 / 2.80
Space group C 1 2 1
Cell size a, b, c (Å), α, β, γ (°) 90.404, 187.4, 108.934, 90, 112.49, 90
R / Rfree (%) 18.9 / 26.5

Zinc Binding Sites:

The binding sites of Zinc atom in the Crystal Structure of Lactobacillus Rhamnosus 4-Deoxy-L-Threo-5- Hexosulose-Uronate Ketol-Isomerase Kdui Complexed with Mops (pdb code 7yrs). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 6 binding sites of Zinc where determined in the Crystal Structure of Lactobacillus Rhamnosus 4-Deoxy-L-Threo-5- Hexosulose-Uronate Ketol-Isomerase Kdui Complexed with Mops, PDB code: 7yrs:
Jump to Zinc binding site number: 1; 2; 3; 4; 5; 6;

Zinc binding site 1 out of 6 in 7yrs

Go back to Zinc Binding Sites List in 7yrs
Zinc binding site 1 out of 6 in the Crystal Structure of Lactobacillus Rhamnosus 4-Deoxy-L-Threo-5- Hexosulose-Uronate Ketol-Isomerase Kdui Complexed with Mops


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Lactobacillus Rhamnosus 4-Deoxy-L-Threo-5- Hexosulose-Uronate Ketol-Isomerase Kdui Complexed with Mops within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn301

b:87.8
occ:1.00
OE2 A:GLU205 1.8 90.2 1.0
OE1 A:GLU205 2.3 90.8 1.0
CD A:GLU205 2.3 84.5 1.0
NE2 A:HIS198 2.4 92.7 1.0
CE1 A:HIS248 2.5 74.1 1.0
NE2 A:HIS200 2.7 93.2 1.0
CD2 A:HIS200 2.9 87.3 1.0
CD2 A:HIS198 3.1 85.7 1.0
NE2 A:HIS248 3.2 75.2 1.0
CE1 A:HIS198 3.5 100.2 1.0
ND1 A:HIS248 3.6 68.9 1.0
CG A:GLU205 3.7 78.1 1.0
CE1 A:HIS200 4.0 86.6 1.0
CG A:HIS200 4.2 82.3 1.0
C5 A:MPO302 4.2 98.1 1.0
CG A:HIS198 4.4 96.3 1.0
CD2 A:HIS248 4.4 74.7 1.0
ND1 A:HIS198 4.5 103.1 1.0
CB A:GLU205 4.5 68.8 1.0
OH A:TYR207 4.6 43.5 1.0
CG A:HIS248 4.7 72.0 1.0
SD A:MET262 4.7 74.2 1.0
ND1 A:HIS200 4.7 82.2 1.0
C6 A:MPO302 4.9 93.8 1.0
O4 A:MPO302 4.9 95.4 1.0

Zinc binding site 2 out of 6 in 7yrs

Go back to Zinc Binding Sites List in 7yrs
Zinc binding site 2 out of 6 in the Crystal Structure of Lactobacillus Rhamnosus 4-Deoxy-L-Threo-5- Hexosulose-Uronate Ketol-Isomerase Kdui Complexed with Mops


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of Lactobacillus Rhamnosus 4-Deoxy-L-Threo-5- Hexosulose-Uronate Ketol-Isomerase Kdui Complexed with Mops within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Zn301

b:108.4
occ:1.00
OE1 B:GLU205 1.9 113.7 1.0
NE2 B:HIS198 2.2 86.5 1.0
OE2 B:GLU205 2.2 101.1 1.0
CD B:GLU205 2.4 90.7 1.0
CE1 B:HIS198 2.8 90.0 1.0
NE2 B:HIS200 3.3 107.9 1.0
CD2 B:HIS198 3.4 90.3 1.0
CE1 B:HIS248 3.5 87.4 1.0
CD2 B:HIS200 3.5 107.5 1.0
ND1 B:HIS248 3.6 83.9 1.0
C6 B:MPO302 3.7 106.7 1.0
CG B:GLU205 3.9 70.4 1.0
CE B:MET262 4.0 80.4 1.0
ND1 B:HIS198 4.0 93.4 1.0
OH B:TYR207 4.1 87.8 1.0
NE2 B:HIS248 4.1 93.7 1.0
CG B:HIS248 4.3 90.4 1.0
CG B:HIS198 4.3 98.0 1.0
C3 B:MPO302 4.4 91.8 1.0
CE1 B:HIS200 4.5 107.9 1.0
O4 B:MPO302 4.5 110.9 1.0
CD2 B:HIS248 4.6 95.3 1.0
C7 B:MPO302 4.6 108.1 1.0
CB B:GLU205 4.7 48.7 1.0
CG B:HIS200 4.8 108.8 1.0

Zinc binding site 3 out of 6 in 7yrs

Go back to Zinc Binding Sites List in 7yrs
Zinc binding site 3 out of 6 in the Crystal Structure of Lactobacillus Rhamnosus 4-Deoxy-L-Threo-5- Hexosulose-Uronate Ketol-Isomerase Kdui Complexed with Mops


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Crystal Structure of Lactobacillus Rhamnosus 4-Deoxy-L-Threo-5- Hexosulose-Uronate Ketol-Isomerase Kdui Complexed with Mops within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Zn301

b:122.7
occ:1.00
OE1 C:GLU205 1.8 116.1 1.0
CD C:GLU205 2.3 105.7 1.0
NE2 C:HIS200 2.3 98.7 1.0
NE2 C:HIS198 2.5 107.8 1.0
OE2 C:GLU205 2.5 113.5 1.0
CD2 C:HIS200 2.6 92.7 1.0
NE2 C:HIS248 2.7 72.8 1.0
CD2 C:HIS198 2.9 110.3 1.0
CE1 C:HIS200 3.5 95.5 1.0
CE1 C:HIS198 3.6 113.6 1.0
CE1 C:HIS248 3.6 63.2 1.0
CE C:MET262 3.6 90.8 1.0
CG C:GLU205 3.6 85.1 1.0
CD2 C:HIS248 3.6 82.8 1.0
CG C:HIS200 3.9 79.9 1.0
CG C:HIS198 4.0 115.9 1.0
ND1 C:HIS200 4.3 86.6 1.0
ND1 C:HIS198 4.4 116.7 1.0
OH C:TYR207 4.6 75.5 1.0
ND1 C:HIS248 4.7 73.5 1.0
CB C:GLU205 4.7 73.8 1.0
CE C:MET272 4.7 102.6 1.0
CG C:HIS248 4.7 82.0 1.0

Zinc binding site 4 out of 6 in 7yrs

Go back to Zinc Binding Sites List in 7yrs
Zinc binding site 4 out of 6 in the Crystal Structure of Lactobacillus Rhamnosus 4-Deoxy-L-Threo-5- Hexosulose-Uronate Ketol-Isomerase Kdui Complexed with Mops


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Crystal Structure of Lactobacillus Rhamnosus 4-Deoxy-L-Threo-5- Hexosulose-Uronate Ketol-Isomerase Kdui Complexed with Mops within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Zn301

b:163.3
occ:1.00
CE1 D:HIS248 2.1 113.3 1.0
OE2 D:GLU205 2.4 101.3 1.0
NE2 D:HIS200 2.5 173.9 1.0
NE2 D:HIS248 2.6 102.9 1.0
CD D:GLU205 2.6 100.3 1.0
OE1 D:GLU205 2.6 110.2 1.0
CD2 D:HIS200 2.7 173.0 1.0
ND1 D:HIS248 3.2 114.8 1.0
CG D:GLU205 3.7 83.9 1.0
CE1 D:HIS200 3.8 173.2 1.0
CD2 D:HIS248 3.8 100.2 1.0
CG D:HIS200 4.0 169.5 1.0
CE D:MET262 4.0 88.1 1.0
CG D:HIS248 4.2 102.3 1.0
OH D:TYR207 4.2 64.0 1.0
ND1 D:HIS200 4.5 171.0 1.0
CB D:GLU205 4.6 65.6 1.0

Zinc binding site 5 out of 6 in 7yrs

Go back to Zinc Binding Sites List in 7yrs
Zinc binding site 5 out of 6 in the Crystal Structure of Lactobacillus Rhamnosus 4-Deoxy-L-Threo-5- Hexosulose-Uronate Ketol-Isomerase Kdui Complexed with Mops


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 5 of Crystal Structure of Lactobacillus Rhamnosus 4-Deoxy-L-Threo-5- Hexosulose-Uronate Ketol-Isomerase Kdui Complexed with Mops within 5.0Å range:
probe atom residue distance (Å) B Occ
E:Zn301

b:134.3
occ:1.00
OE2 E:GLU205 1.8 112.5 1.0
CE1 E:HIS248 2.7 84.5 1.0
CD2 E:HIS200 2.9 139.0 1.0
CD E:GLU205 3.0 96.2 1.0
NE2 E:HIS200 3.0 139.5 1.0
CE E:MET272 3.4 127.3 1.0
NE2 E:HIS248 3.5 68.0 1.0
OE1 E:GLU205 3.6 90.6 1.0
C4 E:MPO302 3.6 109.9 1.0
ND1 E:HIS248 3.7 82.6 1.0
C5 E:MPO302 3.8 110.3 1.0
CB E:HIS198 4.0 142.2 1.0
CG E:HIS200 4.0 134.3 1.0
CG E:GLU205 4.1 87.2 1.0
CE1 E:HIS200 4.2 138.6 1.0
CD2 E:HIS198 4.2 147.6 1.0
SD E:MET272 4.3 131.2 1.0
OH E:TYR207 4.4 56.3 1.0
CG E:HIS198 4.5 145.2 1.0
ND1 E:HIS200 4.7 135.1 1.0
O4 E:MPO302 4.7 119.6 1.0
O E:HIS198 4.7 147.2 1.0
CD2 E:HIS248 4.7 72.0 1.0
CG E:HIS248 4.8 77.5 1.0
SD E:MET262 5.0 123.8 1.0
CB E:HIS200 5.0 131.6 1.0

Zinc binding site 6 out of 6 in 7yrs

Go back to Zinc Binding Sites List in 7yrs
Zinc binding site 6 out of 6 in the Crystal Structure of Lactobacillus Rhamnosus 4-Deoxy-L-Threo-5- Hexosulose-Uronate Ketol-Isomerase Kdui Complexed with Mops


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 6 of Crystal Structure of Lactobacillus Rhamnosus 4-Deoxy-L-Threo-5- Hexosulose-Uronate Ketol-Isomerase Kdui Complexed with Mops within 5.0Å range:
probe atom residue distance (Å) B Occ
F:Zn301

b:110.3
occ:1.00
OE2 F:GLU205 1.7 105.1 1.0
NE2 F:HIS198 2.4 76.9 1.0
NE2 F:HIS248 2.6 86.4 1.0
NE2 F:HIS200 2.7 113.2 1.0
CD F:GLU205 2.8 96.3 1.0
CD2 F:HIS200 2.8 109.2 1.0
CD2 F:HIS198 2.9 73.3 1.0
OE1 F:GLU205 3.3 94.2 1.0
CE1 F:HIS248 3.4 77.3 1.0
CE1 F:HIS198 3.5 84.3 1.0
CD2 F:HIS248 3.6 84.5 1.0
C5 F:MPO302 3.8 103.3 1.0
CG F:GLU205 4.0 87.0 1.0
CE1 F:HIS200 4.0 109.0 1.0
O4 F:MPO302 4.0 108.2 1.0
CG F:HIS198 4.1 75.0 1.0
CG F:HIS200 4.1 102.3 1.0
ND1 F:HIS198 4.4 85.2 1.0
ND1 F:HIS248 4.6 77.6 1.0
ND1 F:HIS200 4.7 105.1 1.0
OH F:TYR207 4.7 49.7 1.0
CG F:HIS248 4.7 80.0 1.0
SD F:MET262 4.8 91.7 1.0
CE F:MET262 4.8 90.6 1.0

Reference:

H.Iwase, Y.Yamamoto, A.Yamada, K.Kawai, S.Oiki, B.Mikami, R.Takase, W.Hashimoto. Lactobacillus Enzymes For Degradation and Metabolism of Glycosaminoglycan Heparin To Be Published.
Page generated: Wed Oct 30 16:02:35 2024

Last articles

Zn in 9JPJ
Zn in 9JP7
Zn in 9JPK
Zn in 9JPL
Zn in 9GN6
Zn in 9GN7
Zn in 9GKU
Zn in 9GKW
Zn in 9GKX
Zn in 9GL0
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy