Zinc in PDB 7xui: Cryo-Em Structure of SIGMA70 Bound HK022 Putrna-Associated E.Coli Rna Polymerase Elongation Complex

All present enzymatic activity of Cryo-Em Structure of SIGMA70 Bound HK022 Putrna-Associated E.Coli Rna Polymerase Elongation Complex:
2.7.7.6;

The structure of Cryo-Em Structure of SIGMA70 Bound HK022 Putrna-Associated E.Coli Rna Polymerase Elongation Complex also contains other interesting chemical elements:

Magnesium

(Mg)

1 atom

The binding sites of Zinc atom in the Cryo-Em Structure of SIGMA70 Bound HK022 Putrna-Associated E.Coli Rna Polymerase Elongation Complex (pdb code 7xui). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Cryo-Em Structure of SIGMA70 Bound HK022 Putrna-Associated E.Coli Rna Polymerase Elongation Complex, PDB code: 7xui:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Cryo-Em Structure of SIGMA70 Bound HK022 Putrna-Associated E.Coli Rna Polymerase Elongation Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Cryo-Em Structure of SIGMA70 Bound HK022 Putrna-Associated E.Coli Rna Polymerase Elongation Complex within 5.0Å range: probe atom residue distance (Å) B Occ J:Zn1501 b:165.0 occ:1.00 SG J:CYS70 2.3 137.0 1.0 SG J:CYS72 2.3 151.3 1.0 SG J:CYS85 2.3 147.8 1.0 SG J:CYS88 2.3 143.8 1.0 CB J:CYS85 3.1 147.8 1.0 CB J:CYS72 3.2 151.3 1.0 N J:CYS88 3.3 143.8 1.0 CB J:CYS88 3.5 143.8 1.0 N J:CYS72 3.5 151.3 1.0 CA J:CYS72 3.9 151.3 1.0 CA J:CYS88 3.9 143.8 1.0 CB J:CYS70 4.0 137.0 1.0 CB J:LYS87 4.2 163.7 1.0 N J:LYS87 4.3 163.7 1.0 C J:LYS87 4.4 163.7 1.0 N J:LEU71 4.4 132.6 1.0 N J:GLY89 4.5 135.1 1.0 C J:CYS88 4.5 143.8 1.0 CA J:CYS85 4.5 147.8 1.0 CA J:LYS87 4.5 163.7 1.0 CB J:LYS74 4.6 165.8 1.0 C J:LEU71 4.7 132.6 1.0 C J:CYS72 4.7 151.3 1.0 C J:CYS70 4.8 137.0 1.0 N J:GLU86 4.8 167.6 1.0 CA J:CYS70 4.9 137.0 1.0 N J:VAL90 5.0 122.7 1.0 N J:GLY73 5.0 153.1 1.0 CA J:LEU71 5.0 132.6 1.0 N J:LYS74 5.0 165.8 1.0

Zinc binding site 2 out of 2 in the Cryo-Em Structure of SIGMA70 Bound HK022 Putrna-Associated E.Coli Rna Polymerase Elongation Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Cryo-Em Structure of SIGMA70 Bound HK022 Putrna-Associated E.Coli Rna Polymerase Elongation Complex within 5.0Å range: probe atom residue distance (Å) B Occ J:Zn1502 b:85.6 occ:1.00 SG J:CYS898 2.3 55.7 1.0 SG J:CYS888 2.3 58.0 1.0 SG J:CYS895 2.3 52.9 1.0 SG J:CYS814 2.3 66.4 1.0 CB J:CYS898 3.0 55.7 1.0 CB J:CYS895 3.3 52.9 1.0 CB J:CYS814 3.4 66.4 1.0 CB J:CYS888 3.5 58.0 1.0 NH2 J:ARG883 3.6 63.1 1.0 CA J:CYS888 4.0 58.0 1.0 N J:CYS814 4.0 66.4 1.0 N J:CYS895 4.1 52.9 1.0 N J:CYS898 4.1 55.7 1.0 CA J:CYS898 4.2 55.7 1.0 CA J:CYS895 4.3 52.9 1.0 N J:ASP889 4.3 67.6 1.0 CA J:CYS814 4.4 66.4 1.0 OG1 J:THR816 4.4 73.4 1.0 CZ J:ARG883 4.6 63.1 1.0 NE J:ARG883 4.6 63.1 1.0 C J:CYS888 4.7 58.0 1.0 OG1 J:THR890 4.8 58.8 1.0 C J:CYS895 4.9 52.9 1.0

S.Hwang, P.D.B.Olinares, J.Lee, J.Kim, B.T.Chait, R.A.King, J.Y.Kang. Structural Basis of Transcriptional Regulation By A Nascent Rna Element, HK022 Putrna. Nat Commun V. 13 4668 2022.
ISSN: ESSN 2041-1723
PubMed: 35970830
DOI: 10.1038/S41467-022-32315-Y