Zinc in PDB 7x7a: Cryo-Em Structure of SBCAS7-11 in Complex with Crrna and Target Rna

The binding sites of Zinc atom in the Cryo-Em Structure of SBCAS7-11 in Complex with Crrna and Target Rna (pdb code 7x7a). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 4 binding sites of Zinc where determined in the Cryo-Em Structure of SBCAS7-11 in Complex with Crrna and Target Rna, PDB code: 7x7a:
Jump to Zinc binding site number: 1; 2; 3; 4;

Zinc binding site 1 out of 4 in the Cryo-Em Structure of SBCAS7-11 in Complex with Crrna and Target Rna


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Cryo-Em Structure of SBCAS7-11 in Complex with Crrna and Target Rna within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1801 b:76.6 occ:1.00 SG A:CYS491 2.3 54.2 1.0 SG A:CYS503 2.3 51.6 1.0 SG A:CYS506 2.3 46.5 1.0 SG A:CYS501 2.3 57.7 1.0 CB A:CYS503 3.1 51.6 1.0 CB A:CYS501 3.3 57.7 1.0 CB A:CYS506 3.3 46.5 1.0 CB A:CYS491 3.8 54.2 1.0 CA A:CYS503 4.3 51.6 1.0 N A:CYS503 4.3 51.6 1.0 N A:CYS491 4.4 54.2 1.0 CA A:CYS501 4.6 57.7 1.0 CA A:CYS491 4.6 54.2 1.0 CA A:CYS506 4.7 46.5 1.0 C A:CYS501 4.7 57.7 1.0 O A:CYS501 4.7 57.7 1.0 N A:CYS506 4.8 46.5 1.0 O A:CYS491 4.8 54.2 1.0

Zinc binding site 2 out of 4 in the Cryo-Em Structure of SBCAS7-11 in Complex with Crrna and Target Rna


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Cryo-Em Structure of SBCAS7-11 in Complex with Crrna and Target Rna within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1802 b:100.9 occ:1.00 SG A:CYS127 2.3 76.3 1.0 SG A:CYS88 2.3 76.5 1.0 SG A:CYS130 2.3 70.8 1.0 SG A:CYS121 2.3 87.6 1.0 CB A:CYS88 3.3 76.5 1.0 CB A:CYS130 3.4 70.8 1.0 CB A:CYS121 3.5 87.6 1.0 CB A:CYS127 3.8 76.3 1.0 N A:CYS127 4.2 76.3 1.0 N A:CYS130 4.2 70.8 1.0 CA A:CYS130 4.4 70.8 1.0 N A:CYS88 4.4 76.5 1.0 CA A:CYS88 4.4 76.5 1.0 CA A:CYS127 4.5 76.3 1.0 NH1 A:ARG135 4.8 66.7 1.0 CA A:CYS121 4.9 87.6 1.0 O A:CYS127 4.9 76.3 1.0

Zinc binding site 3 out of 4 in the Cryo-Em Structure of SBCAS7-11 in Complex with Crrna and Target Rna


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Cryo-Em Structure of SBCAS7-11 in Complex with Crrna and Target Rna within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1803 b:75.0 occ:1.00 CE1 A:HIS747 1.7 61.8 1.0 ND1 A:HIS747 2.1 61.8 1.0 SG A:CYS752 2.3 51.0 1.0 SG A:CYS750 2.3 59.0 1.0 SG A:CYS755 2.3 45.9 1.0 NE2 A:HIS747 2.9 61.8 1.0 CB A:CYS752 3.2 51.0 1.0 CB A:CYS750 3.3 59.0 1.0 CB A:CYS755 3.4 45.9 1.0 CG A:HIS747 3.4 61.8 1.0 CD2 A:HIS747 3.8 61.8 1.0 N A:CYS752 3.9 51.0 1.0 N A:CYS755 4.0 45.9 1.0 CA A:CYS752 4.1 51.0 1.0 CA A:CYS755 4.3 45.9 1.0 NZ A:LYS744 4.3 58.4 1.0 C A:CYS750 4.5 59.0 1.0 CA A:CYS750 4.5 59.0 1.0 CE A:MET754 4.5 44.4 1.0 CB A:HIS747 4.6 61.8 1.0 C A:CYS752 4.7 51.0 1.0 O A:CYS750 4.7 59.0 1.0 N A:THR751 4.8 54.8 1.0 O A:CYS752 4.9 51.0 1.0 CE A:LYS744 4.9 58.4 1.0

Zinc binding site 4 out of 4 in the Cryo-Em Structure of SBCAS7-11 in Complex with Crrna and Target Rna


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Cryo-Em Structure of SBCAS7-11 in Complex with Crrna and Target Rna within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1804 b:62.3 occ:1.00 SG A:CYS1018 2.3 48.8 1.0 SG A:CYS1414 2.3 45.2 1.0 SG A:CYS1417 2.3 39.7 1.0 SG A:CYS1406 2.3 52.8 1.0 CB A:CYS1414 2.6 45.2 1.0 NH1 A:ARG935 3.3 47.5 1.0 CB A:CYS1018 3.5 48.8 1.0 CB A:CYS1417 3.6 39.7 1.0 N A:CYS1414 3.6 45.2 1.0 CB A:CYS1406 3.7 52.8 1.0 CA A:CYS1414 3.7 45.2 1.0 N A:CYS1018 4.0 48.8 1.0 N A:CYS1406 4.2 52.8 1.0 CA A:CYS1018 4.3 48.8 1.0 CA A:CYS1406 4.5 52.8 1.0 CZ A:ARG935 4.5 47.5 1.0 C A:LEU1413 4.8 43.8 1.0 C A:CYS1414 4.9 45.2 1.0 CA A:CYS1417 4.9 39.7 1.0 O A:HIS1404 4.9 58.0 1.0 N A:CYS1417 4.9 39.7 1.0 NH2 A:ARG935 4.9 47.5 1.0 C A:CYS1018 5.0 48.8 1.0

G.Yu, X.Wang, Y.Zhang, Q.An, Y.Wen, X.Li, H.Yin, Z.Deng, H.Zhang. Structure and Function of A Bacterial Type III-E Crispr-CAS7-11 Complex. Nat Microbiol V. 7 2078 2022.
ISSN: ESSN 2058-5276
PubMed: 36302881
DOI: 10.1038/S41564-022-01256-Z