Zinc in PDB 7wah: Structure of CAS7-11 in Complex with Guide Rna and Target Rna

The binding sites of Zinc atom in the Structure of CAS7-11 in Complex with Guide Rna and Target Rna (pdb code 7wah). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 4 binding sites of Zinc where determined in the Structure of CAS7-11 in Complex with Guide Rna and Target Rna, PDB code: 7wah:
Jump to Zinc binding site number: 1; 2; 3; 4;

Zinc binding site 1 out of 4 in the Structure of CAS7-11 in Complex with Guide Rna and Target Rna


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Structure of CAS7-11 in Complex with Guide Rna and Target Rna within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1701 b:122.8 occ:1.00 SG A:CYS86 2.3 74.0 1.0 SG A:CYS123 2.3 70.5 1.0 SG A:CYS126 2.3 60.9 1.0 SG A:CYS115 2.3 96.2 1.0 CB A:CYS86 3.0 70.3 1.0 CB A:CYS115 3.2 93.1 1.0 CB A:CYS126 3.4 63.3 1.0 CB A:CYS123 3.5 70.6 1.0 N A:CYS126 4.0 60.5 1.0 N A:CYS115 4.0 91.9 1.0 CA A:CYS86 4.2 70.1 1.0 N A:CYS86 4.2 68.8 1.0 N A:CYS123 4.2 74.5 1.0 CA A:CYS115 4.2 91.8 1.0 CA A:CYS126 4.2 61.5 1.0 CA A:CYS123 4.4 74.8 1.0 NH1 A:ARG131 4.6 76.5 1.0 O A:CYS123 4.8 77.6 1.0 C A:CYS86 4.9 73.9 1.0 C A:CYS123 4.9 76.5 1.0

Zinc binding site 2 out of 4 in the Structure of CAS7-11 in Complex with Guide Rna and Target Rna


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Structure of CAS7-11 in Complex with Guide Rna and Target Rna within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1702 b:107.3 occ:1.00 SG A:CYS474 2.3 59.0 1.0 SG A:CYS463 2.3 71.4 1.0 SG A:CYS477 2.3 60.9 1.0 SG A:CYS472 2.3 57.7 1.0 CB A:CYS477 3.2 37.5 1.0 CB A:CYS472 3.2 53.4 1.0 CB A:CYS474 3.4 48.1 1.0 CB A:CYS463 3.7 62.0 1.0 N A:CYS477 4.0 32.8 1.0 N A:CYS474 4.1 45.9 1.0 CA A:CYS477 4.1 33.1 1.0 CB A:ALA465 4.2 72.7 1.0 CA A:CYS474 4.3 47.3 1.0 CA A:CYS472 4.5 52.7 1.0 C A:CYS472 4.5 51.7 1.0 CA A:CYS463 4.6 60.5 1.0 NH1 A:ARG452 4.7 26.6 1.0 O A:CYS472 4.8 51.8 1.0 C A:CYS474 4.9 46.5 1.0 N A:MET473 4.9 41.1 1.0

Zinc binding site 3 out of 4 in the Structure of CAS7-11 in Complex with Guide Rna and Target Rna


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Structure of CAS7-11 in Complex with Guide Rna and Target Rna within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1703 b:126.5 occ:1.00 ND1 A:HIS703 2.1 89.3 1.0 SG A:CYS708 2.3 77.3 1.0 SG A:CYS711 2.3 74.1 1.0 SG A:CYS706 2.3 95.7 1.0 CE1 A:HIS703 3.0 90.3 1.0 CG A:HIS703 3.1 86.5 1.0 CA A:HIS703 3.3 89.6 1.0 CB A:HIS703 3.4 87.1 1.0 CB A:CYS711 3.5 63.2 1.0 CB A:CYS708 3.8 78.4 1.0 CB A:CYS706 4.0 94.4 1.0 C A:HIS703 4.1 90.7 1.0 NE2 A:HIS703 4.1 91.2 1.0 CD2 A:HIS703 4.2 87.6 1.0 N A:CYS711 4.3 63.6 1.0 N A:CYS708 4.4 76.8 1.0 O A:HIS703 4.4 90.0 1.0 N A:HIS703 4.4 92.7 1.0 CA A:CYS711 4.5 60.2 1.0 OG A:SER716 4.5 65.8 1.0 CA A:CYS708 4.6 78.1 1.0 O A:CYS706 4.7 93.3 1.0 C A:CYS706 4.8 91.5 1.0 CA A:CYS706 4.9 92.8 1.0 N A:SER704 4.9 91.5 1.0 O A:CYS708 4.9 76.8 1.0

Zinc binding site 4 out of 4 in the Structure of CAS7-11 in Complex with Guide Rna and Target Rna


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Structure of CAS7-11 in Complex with Guide Rna and Target Rna within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1704 b:100.9 occ:1.00 SG A:CYS1345 2.3 60.1 1.0 SG A:CYS1342 2.3 31.2 1.0 SG A:CYS1312 2.3 54.6 1.0 SG A:CYS965 2.3 40.0 1.0 CB A:CYS1345 2.7 37.9 1.0 CB A:CYS965 3.4 35.9 1.0 CB A:CYS1312 3.5 54.2 1.0 CB A:CYS1342 3.7 47.0 1.0 NH1 A:ARG876 3.7 56.9 1.0 N A:CYS1312 3.9 56.1 1.0 CA A:CYS1345 4.0 36.1 1.0 N A:CYS1342 4.1 34.6 1.0 N A:CYS1345 4.2 37.6 1.0 CA A:CYS1312 4.4 57.1 1.0 N A:CYS965 4.5 35.5 1.0 CA A:CYS965 4.5 36.7 1.0 CA A:CYS1342 4.5 43.3 1.0 CG A:ARG967 4.5 33.9 1.0 CD2 A:LEU1341 4.8 50.8 1.0 CZ A:ARG876 4.9 57.7 1.0 C A:PRO1311 5.0 46.6 1.0

K.Kato, W.Zhou, S.Okazaki, Y.Isayama, T.Nishizawa, J.S.Gootenberg, O.O.Abudayyeh, H.Nishimasu. Structure and Engineering of the Type III-E Crispr-CAS7-11 Effector Complex. Cell V. 185 2324 2022.
ISSN: ISSN 1097-4172
PubMed: 35643083
DOI: 10.1016/J.CELL.2022.05.003