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Zinc in PDB 7spz: Nucleotide-Free GET3 in Two Open Forms

Protein crystallography data

The structure of Nucleotide-Free GET3 in Two Open Forms, PDB code: 7spz was solved by M.Y.Fry, A.O.Maggiolo, W.M.Clemons Jr., with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 28.11 / 3.00
Space group P 21 21 2
Cell size a, b, c (Å), α, β, γ (°) 54.138, 102.605, 138.801, 90, 90, 90
R / Rfree (%) 29.3 / 34.8

Zinc Binding Sites:

The binding sites of Zinc atom in the Nucleotide-Free GET3 in Two Open Forms (pdb code 7spz). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Nucleotide-Free GET3 in Two Open Forms, PDB code: 7spz:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in 7spz

Go back to Zinc Binding Sites List in 7spz
Zinc binding site 1 out of 2 in the Nucleotide-Free GET3 in Two Open Forms


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Nucleotide-Free GET3 in Two Open Forms within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn400

b:33.1
occ:0.45
SG A:CYS287 2.2 21.3 1.0
SG A:CYS290 2.3 25.8 1.0
CB A:CYS287 3.3 21.9 1.0
CB A:CYS290 3.5 25.9 1.0
N A:CYS290 4.0 26.4 1.0
CA A:CYS290 4.2 25.9 1.0
CB A:MET289 4.7 29.8 1.0
CA A:CYS287 4.7 22.2 1.0
C A:MET289 4.8 27.3 1.0

Zinc binding site 2 out of 2 in 7spz

Go back to Zinc Binding Sites List in 7spz
Zinc binding site 2 out of 2 in the Nucleotide-Free GET3 in Two Open Forms


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Nucleotide-Free GET3 in Two Open Forms within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Zn400

b:47.9
occ:0.49
SG B:CYS290 2.0 44.5 1.0
SG B:CYS287 2.4 40.0 1.0
CB B:CYS290 3.4 44.9 1.0
CB B:CYS287 3.8 41.3 1.0
N B:CYS290 3.9 45.3 1.0
CA B:CYS290 4.1 44.9 1.0
CB B:MET289 4.7 49.0 1.0
C B:MET289 4.9 46.3 1.0

Reference:

M.Y.Fry, V.Najdrova, A.O.Maggiolo, S.M.Saladi, P.Dolezal, W.M.Clemons Jr.. Structurally Derived Universal Mechanism For the Catalytic Cycle of the Tail-Anchored Targeting Factor GET3. Nat.Struct.Mol.Biol. V. 29 820 2022.
ISSN: ESSN 1545-9985
PubMed: 35851188
DOI: 10.1038/S41594-022-00798-4
Page generated: Sat Apr 8 03:15:16 2023

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