Zinc in PDB 7spz: Nucleotide-Free GET3 in Two Open Forms

The structure of Nucleotide-Free GET3 in Two Open Forms, PDB code: 7spz was solved by M.Y.Fry, A.O.Maggiolo, W.M.Clemons Jr., with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	28.11 / 3.00
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	54.138, 102.605, 138.801, 90, 90, 90
R / Rfree (%)	29.3 / 34.8

The binding sites of Zinc atom in the Nucleotide-Free GET3 in Two Open Forms (pdb code 7spz). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Nucleotide-Free GET3 in Two Open Forms, PDB code: 7spz:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Nucleotide-Free GET3 in Two Open Forms


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Nucleotide-Free GET3 in Two Open Forms within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn400 b:33.1 occ:0.45 SG A:CYS287 2.2 21.3 1.0 SG A:CYS290 2.3 25.8 1.0 CB A:CYS287 3.3 21.9 1.0 CB A:CYS290 3.5 25.9 1.0 N A:CYS290 4.0 26.4 1.0 CA A:CYS290 4.2 25.9 1.0 CB A:MET289 4.7 29.8 1.0 CA A:CYS287 4.7 22.2 1.0 C A:MET289 4.8 27.3 1.0

Zinc binding site 2 out of 2 in the Nucleotide-Free GET3 in Two Open Forms


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Nucleotide-Free GET3 in Two Open Forms within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn400 b:47.9 occ:0.49 SG B:CYS290 2.0 44.5 1.0 SG B:CYS287 2.4 40.0 1.0 CB B:CYS290 3.4 44.9 1.0 CB B:CYS287 3.8 41.3 1.0 N B:CYS290 3.9 45.3 1.0 CA B:CYS290 4.1 44.9 1.0 CB B:MET289 4.7 49.0 1.0 C B:MET289 4.9 46.3 1.0

M.Y.Fry, V.Najdrova, A.O.Maggiolo, S.M.Saladi, P.Dolezal, W.M.Clemons Jr.. Structurally Derived Universal Mechanism For the Catalytic Cycle of the Tail-Anchored Targeting Factor GET3. Nat.Struct.Mol.Biol. V. 29 820 2022.
ISSN: ESSN 1545-9985
PubMed: 35851188
DOI: 10.1038/S41594-022-00798-4