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Zinc in PDB 7s01: X-Ray Structure of the Phage AR9 Non-Virion Rna Polymerase Holoenzyme in Complex with A Forked Oligonucleotide Containing the P077 Promoter

Enzymatic activity of X-Ray Structure of the Phage AR9 Non-Virion Rna Polymerase Holoenzyme in Complex with A Forked Oligonucleotide Containing the P077 Promoter

All present enzymatic activity of X-Ray Structure of the Phage AR9 Non-Virion Rna Polymerase Holoenzyme in Complex with A Forked Oligonucleotide Containing the P077 Promoter:
2.7.7.6;

Protein crystallography data

The structure of X-Ray Structure of the Phage AR9 Non-Virion Rna Polymerase Holoenzyme in Complex with A Forked Oligonucleotide Containing the P077 Promoter, PDB code: 7s01 was solved by P.G.Leiman, M.L.Sokolova, J.Gordeeva, A.Fraser, K.V.Severinov, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 49.35 / 3.40
Space group C 1 2 1
Cell size a, b, c (Å), α, β, γ (°) 176.925, 110.455, 222.379, 90, 98.52, 90
R / Rfree (%) 23.9 / 29.3

Zinc Binding Sites:

The binding sites of Zinc atom in the X-Ray Structure of the Phage AR9 Non-Virion Rna Polymerase Holoenzyme in Complex with A Forked Oligonucleotide Containing the P077 Promoter (pdb code 7s01). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total only one binding site of Zinc was determined in the X-Ray Structure of the Phage AR9 Non-Virion Rna Polymerase Holoenzyme in Complex with A Forked Oligonucleotide Containing the P077 Promoter, PDB code: 7s01:

Zinc binding site 1 out of 1 in 7s01

Go back to Zinc Binding Sites List in 7s01
Zinc binding site 1 out of 1 in the X-Ray Structure of the Phage AR9 Non-Virion Rna Polymerase Holoenzyme in Complex with A Forked Oligonucleotide Containing the P077 Promoter


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of X-Ray Structure of the Phage AR9 Non-Virion Rna Polymerase Holoenzyme in Complex with A Forked Oligonucleotide Containing the P077 Promoter within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Zn801

b:116.1
occ:1.00
SG D:CYS294 2.4 194.8 1.0
SG D:CYS350 2.4 156.8 1.0
SG D:CYS360 2.4 112.4 1.0
SG D:CYS357 2.5 159.4 1.0
CB D:CYS350 3.3 141.8 1.0
CB D:CYS294 3.4 167.6 1.0
CB D:CYS357 3.4 133.1 1.0
CB D:CYS360 3.5 139.8 1.0
CA D:CYS350 3.5 138.3 1.0
N D:CYS357 4.0 137.8 1.0
OG D:SER296 4.1 145.2 1.0
N D:ALA351 4.2 142.3 1.0
CA D:CYS357 4.3 124.3 1.0
N D:CYS360 4.3 131.7 1.0
C D:CYS350 4.4 138.3 1.0
CA D:CYS360 4.5 130.4 1.0
N D:CYS350 4.6 146.9 1.0
CA D:CYS294 4.7 179.5 1.0
CB D:SER296 4.8 131.4 1.0
N D:CYS294 4.9 170.6 1.0
NZ D:LYS359 4.9 132.8 1.0
CD D:LYS359 4.9 132.6 1.0
C D:CYS357 5.0 124.5 1.0

Reference:

A.Fraser, M.L.Sokolova, A.V.Drobysheva, J.V.Gordeeva, S.Borukhov, J.Jumper, K.V.Severinov, P.G.Leiman. Structural Basis of Template Strand Deoxyuridine Promoter Recognition By A Viral Rna Polymerase. Nat Commun V. 13 3526 2022.
ISSN: ESSN 2041-1723
PubMed: 35725571
DOI: 10.1038/S41467-022-31214-6
Page generated: Wed Oct 30 10:35:23 2024

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