Zinc in PDB 7rft: Domain 1 of Starch Adherence System Protein 20 (SAS20) From Ruminococcus Bromii with Maltotriose

The structure of Domain 1 of Starch Adherence System Protein 20 (SAS20) From Ruminococcus Bromii with Maltotriose, PDB code: 7rft was solved by N.Koropatkin, F.Cerqueira, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.52 / 1.53
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	121.837, 64.668, 64.734, 90, 102.76, 90
R / Rfree (%)	17.5 / 19.7

The structure of Domain 1 of Starch Adherence System Protein 20 (SAS20) From Ruminococcus Bromii with Maltotriose also contains other interesting chemical elements:

Chlorine

(Cl)

2 atoms

The binding sites of Zinc atom in the Domain 1 of Starch Adherence System Protein 20 (SAS20) From Ruminococcus Bromii with Maltotriose (pdb code 7rft). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Domain 1 of Starch Adherence System Protein 20 (SAS20) From Ruminococcus Bromii with Maltotriose, PDB code: 7rft:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Domain 1 of Starch Adherence System Protein 20 (SAS20) From Ruminococcus Bromii with Maltotriose


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Domain 1 of Starch Adherence System Protein 20 (SAS20) From Ruminococcus Bromii with Maltotriose within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn305 b:16.6 occ:0.50 ND1 B:HIS182 1.9 17.5 0.5 ND1 A:HIS182 1.9 18.1 0.5 OE1 A:GLU150 2.0 18.1 1.0 O B:HOH590 2.1 14.3 1.0 CE1 B:HIS182 2.8 17.5 0.5 CG A:HIS182 2.9 18.0 0.5 CE1 A:HIS182 3.0 18.3 0.5 CD A:GLU150 3.0 17.8 1.0 CG B:HIS182 3.1 17.6 0.5 CB A:HIS182 3.1 17.6 0.5 CG A:HIS182 3.1 17.5 0.5 CB A:HIS182 3.1 17.8 0.5 CD2 A:HIS182 3.2 17.4 0.5 CB B:HIS182 3.5 17.7 0.5 CB B:HIS182 3.6 17.6 0.5 OE2 A:GLU150 3.6 17.7 1.0 CG B:HIS182 3.6 17.7 0.5 CA B:HIS182 3.7 17.8 0.5 CA B:HIS182 3.7 17.7 0.5 CD2 B:HIS182 3.8 17.9 0.5 N B:VAL183 3.9 17.3 1.0 ND1 A:HIS182 3.9 17.5 0.5 NE2 B:HIS182 4.0 17.7 0.5 CD2 A:HIS182 4.0 18.2 0.5 NE2 A:HIS182 4.1 17.4 0.5 NE2 A:HIS182 4.1 18.4 0.5 O B:HOH484 4.1 28.0 1.0 CD2 B:HIS182 4.1 17.5 0.5 CG A:GLU150 4.2 17.4 1.0 CD1 A:LEU180 4.2 17.7 1.0 C B:HIS182 4.3 17.6 1.0 CB A:GLU150 4.4 17.1 1.0 CE1 A:HIS182 4.4 17.6 0.5 ND1 B:HIS182 4.5 17.8 0.5 CA A:HIS182 4.6 17.8 0.5 O B:VAL183 4.6 17.1 1.0 CA A:HIS182 4.6 17.9 0.5 NE2 B:HIS182 4.7 17.9 0.5 CB A:LEU180 4.8 17.6 1.0 OG1 A:THR178 4.8 16.4 1.0 N B:HIS182 5.0 17.8 1.0

Zinc binding site 2 out of 2 in the Domain 1 of Starch Adherence System Protein 20 (SAS20) From Ruminococcus Bromii with Maltotriose


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Domain 1 of Starch Adherence System Protein 20 (SAS20) From Ruminococcus Bromii with Maltotriose within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn303 b:18.1 occ:0.50 ND1 B:HIS182 2.0 17.8 0.5 OE1 B:GLU150 2.0 19.0 1.0 ND1 A:HIS182 2.0 17.5 0.5 O A:HOH633 2.1 14.7 1.0 CG B:HIS182 2.9 17.7 0.5 CE1 A:HIS182 2.9 17.6 0.5 CE1 B:HIS182 3.0 17.9 0.5 CD B:GLU150 3.1 18.5 1.0 CB B:HIS182 3.1 17.6 0.5 CB B:HIS182 3.1 17.7 0.5 CG B:HIS182 3.1 17.6 0.5 CG A:HIS182 3.1 17.5 0.5 CD2 B:HIS182 3.2 17.5 0.5 CB A:HIS182 3.4 17.8 0.5 CB A:HIS182 3.6 17.6 0.5 OE2 B:GLU150 3.6 18.8 1.0 CG A:HIS182 3.7 18.0 0.5 CA A:HIS182 3.7 17.9 0.5 CA A:HIS182 3.7 17.8 0.5 N A:VAL183 3.9 17.6 1.0 CD2 A:HIS182 3.9 18.2 0.5 ND1 B:HIS182 4.0 17.5 0.5 CD2 B:HIS182 4.0 17.9 0.5 O A:HOH478 4.1 28.0 1.0 NE2 B:HIS182 4.1 17.9 0.5 NE2 A:HIS182 4.1 17.4 0.5 NE2 B:HIS182 4.1 17.7 0.5 CD1 B:LEU180 4.1 18.9 1.0 CG B:GLU150 4.2 18.3 1.0 CD2 A:HIS182 4.2 17.4 0.5 C A:HIS182 4.3 17.6 1.0 CB B:GLU150 4.4 17.9 1.0 CE1 B:HIS182 4.5 17.5 0.5 ND1 A:HIS182 4.5 18.1 0.5 CA B:HIS182 4.6 17.7 0.5 CA B:HIS182 4.6 17.8 0.5 O A:VAL183 4.7 18.2 1.0 CB B:LEU180 4.8 18.4 1.0 NE2 A:HIS182 4.8 18.4 0.5 OG1 B:THR178 4.8 17.2 1.0

F.M.Cerqueira, A.L.Photenhauer, H.L.Doden, A.N.Brown, A.M.Abdel-Hamid, S.Morais, E.A.Bayer, Z.Wawrzak, I.Cann, J.M.Ridlon, J.B.Hopkins, N.M.Koropatkin. SAS20 Is A Highly Flexible Starch-Binding Protein in the Ruminococcus Bromii Cell-Surface Amylosome. J.Biol.Chem. V. 298 01896 2022.
ISSN: ESSN 1083-351X
PubMed: 35378131
DOI: 10.1016/J.JBC.2022.101896