Zinc in PDB 7re5: Crystal Structure of the Tetramerization Domain (29-147) From Human Voltage-Gated Potassium Channel KV2.1 in P 41 21 2 Space Group

The structure of Crystal Structure of the Tetramerization Domain (29-147) From Human Voltage-Gated Potassium Channel KV2.1 in P 41 21 2 Space Group, PDB code: 7re5 was solved by Z.Xu, N.Schnicker, S.Baker, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	44.37 / 2.50
Space group	P 41 21 2
Cell size a, b, c (Å), α, β, γ (°)	78.754, 78.754, 214.803, 90, 90, 90
R / Rfree (%)	22.5 / 26.8

The binding sites of Zinc atom in the Crystal Structure of the Tetramerization Domain (29-147) From Human Voltage-Gated Potassium Channel KV2.1 in P 41 21 2 Space Group (pdb code 7re5). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 8 binding sites of Zinc where determined in the Crystal Structure of the Tetramerization Domain (29-147) From Human Voltage-Gated Potassium Channel KV2.1 in P 41 21 2 Space Group, PDB code: 7re5:
Jump to Zinc binding site number: 1; 2; 3; 4; 5; 6; 7; 8;

Zinc binding site 1 out of 8 in the Crystal Structure of the Tetramerization Domain (29-147) From Human Voltage-Gated Potassium Channel KV2.1 in P 41 21 2 Space Group


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of the Tetramerization Domain (29-147) From Human Voltage-Gated Potassium Channel KV2.1 in P 41 21 2 Space Group within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn201 b:78.8 occ:1.00 SG A:CYS133 1.9 75.8 1.0 ND1 A:HIS105 2.1 68.0 1.0 SG B:CYS111 2.2 62.4 1.0 SG A:CYS132 2.7 76.1 1.0 CE1 A:HIS105 3.0 67.9 1.0 CG A:HIS105 3.1 61.8 1.0 CB B:CYS111 3.4 59.4 1.0 CB A:HIS105 3.5 54.5 1.0 CB A:CYS133 3.6 71.9 1.0 N A:CYS133 3.6 75.1 1.0 CB A:CYS132 3.7 74.0 1.0 NE2 A:HIS105 4.1 61.7 1.0 CA A:HIS105 4.1 59.4 1.0 CA A:CYS133 4.2 76.6 1.0 C A:CYS132 4.2 74.8 1.0 CD2 A:HIS105 4.2 58.9 1.0 CB A:GLU130 4.3 64.3 1.0 CA A:CYS132 4.5 73.8 1.0 N A:MET106 4.6 64.1 1.0 CA B:CYS111 4.8 63.2 1.0 O A:MET106 4.8 71.1 1.0 N A:CYS132 4.8 72.5 1.0 C A:HIS105 4.9 61.1 1.0

Zinc binding site 2 out of 8 in the Crystal Structure of the Tetramerization Domain (29-147) From Human Voltage-Gated Potassium Channel KV2.1 in P 41 21 2 Space Group


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of the Tetramerization Domain (29-147) From Human Voltage-Gated Potassium Channel KV2.1 in P 41 21 2 Space Group within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn202 b:52.2 occ:0.50 NE2 A:HIS27 2.1 56.3 1.0 SG A:CYS29 2.3 58.5 1.0 CD2 A:HIS27 2.9 52.0 1.0 CB A:CYS29 3.2 41.8 1.0 CE1 A:HIS27 3.3 55.4 1.0 CG A:HIS27 4.1 53.5 1.0 ND1 A:HIS27 4.2 61.2 1.0 CA A:CYS29 4.4 40.9 1.0 NH2 A:ARG61 4.6 49.2 1.0

Zinc binding site 3 out of 8 in the Crystal Structure of the Tetramerization Domain (29-147) From Human Voltage-Gated Potassium Channel KV2.1 in P 41 21 2 Space Group


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Crystal Structure of the Tetramerization Domain (29-147) From Human Voltage-Gated Potassium Channel KV2.1 in P 41 21 2 Space Group within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn201 b:61.7 occ:1.00 SG B:CYS132 1.9 73.7 1.0 ND1 B:HIS105 2.1 53.2 1.0 SG C:CYS111 2.2 56.4 1.0 SG B:CYS133 2.4 64.2 1.0 CE1 B:HIS105 3.0 56.5 1.0 CG B:HIS105 3.1 59.4 1.0 CB B:CYS132 3.2 61.6 1.0 CB C:CYS111 3.3 54.8 1.0 CB B:HIS105 3.4 50.8 1.0 N B:CYS133 3.7 67.9 1.0 CB B:CYS133 3.8 66.7 1.0 C B:CYS132 4.0 69.3 1.0 CA B:HIS105 4.1 53.3 1.0 NE2 B:HIS105 4.1 56.8 1.0 CA B:CYS132 4.2 66.0 1.0 CA B:CYS133 4.2 71.6 1.0 CD2 B:HIS105 4.2 56.8 1.0 CB B:GLU130 4.4 54.1 1.0 N B:MET106 4.6 59.7 1.0 CA C:CYS111 4.7 56.9 1.0 N B:CYS132 4.7 65.8 1.0 O B:CYS132 4.8 70.8 1.0 C B:HIS105 4.9 55.2 1.0

Zinc binding site 4 out of 8 in the Crystal Structure of the Tetramerization Domain (29-147) From Human Voltage-Gated Potassium Channel KV2.1 in P 41 21 2 Space Group


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Crystal Structure of the Tetramerization Domain (29-147) From Human Voltage-Gated Potassium Channel KV2.1 in P 41 21 2 Space Group within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn202 b:61.5 occ:1.00 NE2 B:HIS27 2.1 60.9 1.0 SG B:CYS29 2.8 73.2 1.0 CD2 B:HIS27 3.0 64.8 1.0 CE1 B:HIS27 3.1 63.3 1.0 CB B:CYS29 3.1 61.9 1.0 ND1 B:HIS27 4.2 66.8 1.0 CG B:HIS27 4.2 68.0 1.0 CA B:CYS29 4.5 60.8 1.0 NH2 B:ARG61 4.9 59.5 1.0

Zinc binding site 5 out of 8 in the Crystal Structure of the Tetramerization Domain (29-147) From Human Voltage-Gated Potassium Channel KV2.1 in P 41 21 2 Space Group


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 5 of Crystal Structure of the Tetramerization Domain (29-147) From Human Voltage-Gated Potassium Channel KV2.1 in P 41 21 2 Space Group within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn201 b:76.0 occ:1.00 SG C:CYS133 1.8 73.2 1.0 ND1 C:HIS105 2.0 70.7 1.0 SG D:CYS111 2.2 64.3 1.0 SG C:CYS132 3.0 80.7 1.0 CE1 C:HIS105 3.0 69.5 1.0 CB D:CYS111 3.0 62.9 1.0 CG C:HIS105 3.1 67.3 1.0 CB C:HIS105 3.4 55.2 1.0 CB C:CYS133 3.5 74.0 1.0 N C:CYS133 3.7 69.8 1.0 CB C:CYS132 3.9 75.5 1.0 NE2 C:HIS105 4.1 67.1 1.0 CA C:HIS105 4.2 54.0 1.0 CD2 C:HIS105 4.2 66.0 1.0 CA C:CYS133 4.2 76.6 1.0 C C:CYS132 4.3 72.3 1.0 CB C:GLU130 4.4 69.3 1.0 CA D:CYS111 4.5 63.6 1.0 N C:MET106 4.6 60.8 1.0 CA C:CYS132 4.6 71.7 1.0 C C:HIS105 5.0 60.7 1.0

Zinc binding site 6 out of 8 in the Crystal Structure of the Tetramerization Domain (29-147) From Human Voltage-Gated Potassium Channel KV2.1 in P 41 21 2 Space Group


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 6 of Crystal Structure of the Tetramerization Domain (29-147) From Human Voltage-Gated Potassium Channel KV2.1 in P 41 21 2 Space Group within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn202 b:63.4 occ:1.00 NE2 C:HIS27 2.2 57.2 1.0 CB C:CYS29 2.7 48.1 1.0 CE1 C:HIS27 2.9 67.1 1.0 SG C:CYS29 2.9 79.4 1.0 CD2 C:HIS27 3.3 66.9 1.0 CA C:CYS29 3.9 51.4 1.0 ND1 C:HIS27 4.0 69.6 1.0 CG C:HIS27 4.2 67.1 1.0 N C:CYS29 4.5 55.5 1.0

Zinc binding site 7 out of 8 in the Crystal Structure of the Tetramerization Domain (29-147) From Human Voltage-Gated Potassium Channel KV2.1 in P 41 21 2 Space Group


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 7 of Crystal Structure of the Tetramerization Domain (29-147) From Human Voltage-Gated Potassium Channel KV2.1 in P 41 21 2 Space Group within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn201 b:83.3 occ:1.00 SG D:CYS133 2.0 79.3 1.0 ND1 D:HIS105 2.1 80.7 1.0 SG E:CYS111 2.2 63.4 1.0 SG D:CYS132 2.6 81.5 1.0 CB D:CYS133 2.9 89.4 1.0 CG D:HIS105 3.0 75.5 1.0 N D:CYS133 3.1 93.8 1.0 CE1 D:HIS105 3.2 81.3 1.0 CB D:HIS105 3.3 69.9 1.0 CA D:CYS133 3.5 95.4 1.0 CB E:CYS111 3.6 74.7 1.0 CB D:CYS132 3.9 90.8 1.0 CA D:HIS105 4.0 69.4 1.0 C D:CYS132 4.0 90.0 1.0 CD2 D:HIS105 4.2 74.2 1.0 NE2 D:HIS105 4.2 77.1 1.0 N D:MET106 4.5 69.8 1.0 CA D:CYS132 4.5 88.1 1.0 C D:HIS105 4.8 73.7 1.0 CA E:CYS111 4.9 70.4 1.0 CB D:GLU130 4.9 82.4 1.0 O D:CYS132 5.0 93.7 1.0 C D:CYS133 5.0 91.2 1.0

Zinc binding site 8 out of 8 in the Crystal Structure of the Tetramerization Domain (29-147) From Human Voltage-Gated Potassium Channel KV2.1 in P 41 21 2 Space Group


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 8 of Crystal Structure of the Tetramerization Domain (29-147) From Human Voltage-Gated Potassium Channel KV2.1 in P 41 21 2 Space Group within 5.0Å range: probe atom residue distance (Å) B Occ E:Zn201 b:81.6 occ:1.00 SG E:CYS133 2.1 98.6 1.0 SG A:CYS111 2.2 61.9 1.0 SG E:CYS132 2.4 91.1 1.0 ND1 E:HIS105 2.8 75.2 1.0 CB A:CYS111 3.0 64.6 1.0 CB E:CYS133 3.1 78.0 1.0 N E:CYS133 3.2 90.8 1.0 CB E:CYS132 3.2 73.0 1.0 CB E:HIS105 3.5 72.5 1.0 C E:CYS132 3.5 82.5 1.0 CG E:HIS105 3.6 77.7 1.0 CA E:CYS133 3.7 83.5 1.0 CA E:CYS132 3.9 74.3 1.0 CE1 E:HIS105 3.9 77.4 1.0 CA E:HIS105 4.1 70.6 1.0 O E:CYS132 4.1 79.9 1.0 N E:CYS132 4.4 76.3 1.0 CB E:GLU130 4.5 78.8 1.0 CA A:CYS111 4.5 66.7 1.0 CD2 E:HIS105 4.8 79.0 1.0 N E:MET106 4.8 73.7 1.0 C E:HIS105 4.9 78.7 1.0 NE2 E:HIS105 5.0 80.9 1.0

Z.Xu, S.Khan, N.J.Schnicker, S.Baker. Pentameric Assembly of the KV2.1 Tetramerization Domain. Acta Crystallogr D Struct V. 78 792 2022BIOL.
ISSN: ISSN 2059-7983
PubMed: 35647925
DOI: 10.1107/S205979832200568X