Zinc in PDB 7nq4: Human Trna Guanine Transglycosylase (Tgt), Rna-Bound Covalent Intermediate

All present enzymatic activity of Human Trna Guanine Transglycosylase (Tgt), Rna-Bound Covalent Intermediate:
2.4.2.29;

The structure of Human Trna Guanine Transglycosylase (Tgt), Rna-Bound Covalent Intermediate, PDB code: 7nq4 was solved by K.Sievers, R.Ficner, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	42.20 / 2.88
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	161.84, 56.96, 102.96, 90, 124.94, 90
R / Rfree (%)	21.2 / 24.8

The binding sites of Zinc atom in the Human Trna Guanine Transglycosylase (Tgt), Rna-Bound Covalent Intermediate (pdb code 7nq4). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Human Trna Guanine Transglycosylase (Tgt), Rna-Bound Covalent Intermediate, PDB code: 7nq4:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Human Trna Guanine Transglycosylase (Tgt), Rna-Bound Covalent Intermediate


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Human Trna Guanine Transglycosylase (Tgt), Rna-Bound Covalent Intermediate within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn501 b:60.2 occ:1.00 ND1 A:HIS348 2.0 60.1 1.0 SG A:CYS317 2.3 66.7 1.0 SG A:CYS319 2.3 62.8 1.0 SG A:CYS322 2.3 66.4 1.0 CE1 A:HIS348 2.9 62.0 1.0 CG A:HIS348 3.1 56.8 1.0 CB A:CYS317 3.2 72.1 1.0 CB A:CYS322 3.3 64.4 1.0 CB A:CYS319 3.5 61.4 1.0 CB A:HIS348 3.5 55.2 1.0 NE2 A:HIS348 4.0 60.8 1.0 CA A:HIS348 4.1 59.5 1.0 N A:CYS322 4.1 64.4 1.0 CD2 A:HIS348 4.1 57.1 1.0 N A:CYS319 4.3 67.2 1.0 CA A:CYS322 4.3 65.1 1.0 CA A:CYS319 4.3 64.8 1.0 O A:CYS319 4.5 72.5 1.0 CA A:CYS317 4.5 70.3 1.0 O A:HIS348 4.7 60.2 1.0 C A:CYS319 4.7 66.9 1.0 CB A:THR321 4.8 61.0 1.0 C A:CYS317 4.8 71.6 1.0 C A:HIS348 4.8 59.3 1.0

Zinc binding site 2 out of 2 in the Human Trna Guanine Transglycosylase (Tgt), Rna-Bound Covalent Intermediate


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Human Trna Guanine Transglycosylase (Tgt), Rna-Bound Covalent Intermediate within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn501 b:83.8 occ:1.00 ND1 B:HIS382 2.1 83.1 1.0 SG B:CYS353 2.3 82.3 1.0 SG B:CYS356 2.3 90.2 1.0 SG B:CYS351 2.3 89.7 1.0 CE1 B:HIS382 3.0 83.6 1.0 CG B:HIS382 3.1 82.0 1.0 CB B:CYS353 3.2 81.2 1.0 CB B:CYS356 3.2 88.2 1.0 CB B:CYS351 3.3 85.8 1.0 CB B:HIS382 3.5 76.9 1.0 N B:CYS356 3.8 79.9 1.0 CA B:HIS382 4.1 77.7 1.0 CA B:CYS356 4.1 84.1 1.0 CA B:CYS353 4.1 80.0 1.0 NE2 B:HIS382 4.1 85.6 1.0 N B:CYS353 4.1 84.3 1.0 CD2 B:HIS382 4.2 82.5 1.0 O B:CYS351 4.4 94.2 1.0 C B:CYS351 4.5 87.5 1.0 CA B:CYS351 4.5 85.2 1.0 O B:CYS353 4.6 73.5 1.0 C B:CYS353 4.6 75.3 1.0 O B:HIS382 4.7 78.7 1.0 C B:HIS382 4.9 77.0 1.0 C B:CYS355 5.0 80.5 1.0

K.Sievers, L.Welp, H.Urlaub, R.Ficner. Structural and Functional Insights Into Human Trna Guanine Transgylcosylase Rna Biol. 2021.
ISSN: ESSN 1555-8584
DOI: 10.1080/15476286.2021.1950980