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Zinc in PDB 7n4e: Escherichia Coli Sigma 70-Dependent Paused Transcription Elongation Complex

Enzymatic activity of Escherichia Coli Sigma 70-Dependent Paused Transcription Elongation Complex

All present enzymatic activity of Escherichia Coli Sigma 70-Dependent Paused Transcription Elongation Complex:
2.7.7.6;

Other elements in 7n4e:

The structure of Escherichia Coli Sigma 70-Dependent Paused Transcription Elongation Complex also contains other interesting chemical elements:

Magnesium (Mg) 1 atom

Zinc Binding Sites:

The binding sites of Zinc atom in the Escherichia Coli Sigma 70-Dependent Paused Transcription Elongation Complex (pdb code 7n4e). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Escherichia Coli Sigma 70-Dependent Paused Transcription Elongation Complex, PDB code: 7n4e:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in 7n4e

Go back to Zinc Binding Sites List in 7n4e
Zinc binding site 1 out of 2 in the Escherichia Coli Sigma 70-Dependent Paused Transcription Elongation Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Escherichia Coli Sigma 70-Dependent Paused Transcription Elongation Complex within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Zn1501

b:150.1
occ:1.00
N D:CYS72 2.0 98.1 1.0
SG D:CYS72 2.3 98.1 1.0
N D:GLY73 2.4 104.1 1.0
CB D:CYS70 2.4 94.3 1.0
N D:LEU71 2.6 92.6 1.0
CA D:CYS72 2.7 98.1 1.0
C D:CYS70 2.7 94.3 1.0
C D:LEU71 2.8 92.6 1.0
C D:CYS72 2.9 98.1 1.0
CB D:CYS72 2.9 98.1 1.0
CA D:CYS70 3.1 94.3 1.0
CA D:LEU71 3.2 92.6 1.0
O D:CYS70 3.3 94.3 1.0
CA D:GLY73 3.5 104.1 1.0
SG D:CYS88 3.6 87.1 1.0
N D:LYS74 3.7 96.3 1.0
O D:LEU71 3.8 92.6 1.0
SG D:CYS70 4.0 94.3 1.0
O D:CYS72 4.1 98.1 1.0
C D:GLY73 4.1 104.1 1.0
N D:CYS70 4.1 94.3 1.0
CB D:LEU71 4.2 92.6 1.0
CA D:LYS74 4.9 96.3 1.0
CB D:CYS88 5.0 87.1 1.0
CG1 D:VAL90 5.0 67.0 1.0

Zinc binding site 2 out of 2 in 7n4e

Go back to Zinc Binding Sites List in 7n4e
Zinc binding site 2 out of 2 in the Escherichia Coli Sigma 70-Dependent Paused Transcription Elongation Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Escherichia Coli Sigma 70-Dependent Paused Transcription Elongation Complex within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Zn1502

b:148.6
occ:1.00
O D:SER884 2.0 51.2 1.0
SG D:CYS898 2.3 47.3 1.0
CB D:CYS888 2.4 54.2 1.0
CB D:CYS898 2.5 47.3 1.0
CA D:CYS888 2.8 54.2 1.0
N D:CYS888 2.9 54.2 1.0
C D:SER884 3.0 51.2 1.0
CA D:VAL885 3.4 44.4 1.0
N D:VAL885 3.6 44.4 1.0
O D:VAL885 3.6 44.4 1.0
C D:VAL885 3.7 44.4 1.0
C D:SER887 3.8 52.5 1.0
SG D:CYS888 3.8 54.2 1.0
CA D:CYS898 3.8 47.3 1.0
OG D:SER887 4.2 52.5 1.0
N D:SER884 4.2 51.2 1.0
CA D:SER884 4.2 51.2 1.0
C D:CYS888 4.3 54.2 1.0
N D:SER887 4.3 52.5 1.0
O D:SER887 4.4 52.5 1.0
C D:CYS898 4.5 47.3 1.0
CA D:SER887 4.5 52.5 1.0
N D:VAL886 4.6 41.9 1.0
O D:CYS898 4.7 47.3 1.0
CB D:VAL885 4.8 44.4 1.0
C D:ARG883 4.9 53.4 1.0
N D:CYS898 4.9 47.3 1.0
N D:ASP889 5.0 52.6 1.0
CB D:SER887 5.0 52.5 1.0

Reference:

C.Pukhrambam, V.Molodtsov, M.Kooshkbaghi, A.Tareen, H.Vu, K.S.Skalenko, M.Su, Z.Yin, J.T.Winkelman, J.B.Kinney, R.H.Ebright, B.E.Nickels. Structural and Mechanistic Basis of Sigma-Dependent Transcriptional Pausing. Proc.Natl.Acad.Sci.Usa V. 119 01119 2022.
ISSN: ESSN 1091-6490
PubMed: 35653571
DOI: 10.1073/PNAS.2201301119
Page generated: Wed Oct 30 07:39:35 2024

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