Zinc in PDB 7mlj: Crystal Structure of Thermus Thermophilus Reiterative Transcription Complex with 4NT Oligo-G Rna

All present enzymatic activity of Crystal Structure of Thermus Thermophilus Reiterative Transcription Complex with 4NT Oligo-G Rna:
2.7.7.6;

The structure of Crystal Structure of Thermus Thermophilus Reiterative Transcription Complex with 4NT Oligo-G Rna, PDB code: 7mlj was solved by Y.Liu, R.H.Ebright, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	46.21 / 3.75
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	186.309, 104.656, 298.413, 90, 98.12, 90
R / Rfree (%)	26.5 / 28.3

The structure of Crystal Structure of Thermus Thermophilus Reiterative Transcription Complex with 4NT Oligo-G Rna also contains other interesting chemical elements:

Magnesium

(Mg)

5 atoms

The binding sites of Zinc atom in the Crystal Structure of Thermus Thermophilus Reiterative Transcription Complex with 4NT Oligo-G Rna (pdb code 7mlj). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of Thermus Thermophilus Reiterative Transcription Complex with 4NT Oligo-G Rna, PDB code: 7mlj:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of Thermus Thermophilus Reiterative Transcription Complex with 4NT Oligo-G Rna


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Thermus Thermophilus Reiterative Transcription Complex with 4NT Oligo-G Rna within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn2001 b:123.0 occ:1.00 SG D:CYS1201 2.3 113.0 1.0 SG D:CYS1204 2.3 103.1 1.0 SG D:CYS1194 2.3 133.6 1.0 SG D:CYS1112 2.3 125.7 1.0 CB D:CYS1204 3.0 119.7 1.0 CB D:CYS1194 3.3 119.7 1.0 CB D:CYS1112 3.5 127.6 1.0 CA D:CYS1194 3.6 117.1 1.0 CB D:CYS1201 3.7 128.0 1.0 OG1 D:THR1196 3.8 133.1 1.0 N D:GLN1195 4.1 130.2 1.0 N D:CYS1112 4.1 140.2 1.0 N D:CYS1201 4.2 127.8 1.0 CA D:CYS1204 4.2 123.5 1.0 N D:CYS1204 4.3 126.1 1.0 NH2 D:ARG1189 4.3 129.4 1.0 C D:CYS1194 4.4 122.9 1.0 CG2 D:THR1114 4.4 125.6 1.0 CA D:CYS1112 4.4 133.1 1.0 CA D:CYS1201 4.5 134.4 1.0 N D:THR1196 4.7 125.6 1.0 N D:CYS1194 4.8 109.5 1.0 CB D:THR1196 4.9 136.4 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of Thermus Thermophilus Reiterative Transcription Complex with 4NT Oligo-G Rna


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of Thermus Thermophilus Reiterative Transcription Complex with 4NT Oligo-G Rna within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn2002 b:197.6 occ:1.00 SG D:CYS76 2.3 177.7 1.0 SG D:CYS60 2.3 180.1 1.0 SG D:CYS58 2.3 177.0 1.0 SG D:CYS73 2.3 190.1 1.0 CB D:CYS73 3.1 172.1 1.0 CB D:CYS58 3.1 177.9 1.0 CB D:CYS76 3.5 156.9 1.0 N D:CYS60 3.7 177.3 1.0 CB D:CYS60 3.7 175.1 1.0 N D:CYS76 3.9 171.8 1.0 N D:LYS62 4.0 187.5 1.0 N D:GLY61 4.0 185.4 1.0 CB D:LYS62 4.1 185.1 1.0 N D:ALA59 4.1 174.0 1.0 CA D:CYS60 4.1 177.6 1.0 CA D:CYS76 4.3 163.0 1.0 CA D:CYS58 4.4 174.9 1.0 C D:CYS58 4.4 173.4 1.0 C D:CYS60 4.5 182.2 1.0 CA D:CYS73 4.5 172.1 1.0 CA D:LYS62 4.6 187.9 1.0 C D:ALA59 4.7 177.0 1.0 CD1 D:TYR63 4.8 179.1 1.0 CB D:ARG75 4.8 167.3 1.0 CA D:ALA59 4.9 173.8 1.0 N D:TYR63 5.0 186.8 1.0 C D:GLY61 5.0 190.4 1.0 CA D:GLY61 5.0 187.0 1.0

Y.Liu, L.Yu, C.Pukhrambam, J.T.Winkelman, E.Firlar, J.T.Kaelber, Y.Zhang, B.E.Nickels, R.H.Ebright. Structural and Mechanistic Basis of Reiterative Transcription Initiation. Proc.Natl.Acad.Sci.Usa V. 119 2022.
ISSN: ESSN 1091-6490
PubMed: 35082149
DOI: 10.1073/PNAS.2115746119