Zinc in PDB 7mkj: Cryo-Em Structure of Escherichia Coli Rna Polymerase Bound to T7A1 Promoter Dna

All present enzymatic activity of Cryo-Em Structure of Escherichia Coli Rna Polymerase Bound to T7A1 Promoter Dna:
2.7.7.6;

The structure of Cryo-Em Structure of Escherichia Coli Rna Polymerase Bound to T7A1 Promoter Dna also contains other interesting chemical elements:

Magnesium

(Mg)

1 atom

The binding sites of Zinc atom in the Cryo-Em Structure of Escherichia Coli Rna Polymerase Bound to T7A1 Promoter Dna (pdb code 7mkj). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Cryo-Em Structure of Escherichia Coli Rna Polymerase Bound to T7A1 Promoter Dna, PDB code: 7mkj:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Cryo-Em Structure of Escherichia Coli Rna Polymerase Bound to T7A1 Promoter Dna


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Cryo-Em Structure of Escherichia Coli Rna Polymerase Bound to T7A1 Promoter Dna within 5.0Å range: probe atom residue distance (Å) B Occ J:Zn1502 b:114.6 occ:1.00 SG J:CYS72 2.3 69.2 1.0 SG J:CYS85 2.3 77.6 1.0 SG J:CYS70 2.3 66.3 1.0 CB J:CYS85 2.4 77.6 1.0 SG J:CYS88 3.1 75.0 1.0 CB J:CYS70 3.4 66.3 1.0 CA J:CYS85 3.9 77.6 1.0 CB J:CYS72 4.0 69.2 1.0 CB J:LYS74 4.2 72.3 1.0 N J:CYS88 4.3 75.0 1.0 N J:CYS72 4.3 69.2 1.0 CD1 J:TYR75 4.4 69.1 1.0 CB J:CYS88 4.4 75.0 1.0 N J:LYS74 4.5 72.3 1.0 CB J:LYS87 4.5 88.9 1.0 N J:GLY73 4.6 70.3 1.0 N J:LEU71 4.7 60.4 1.0 CA J:CYS70 4.7 66.3 1.0 C J:CYS85 4.7 77.6 1.0 CE1 J:TYR75 4.7 69.1 1.0 CA J:CYS72 4.7 69.2 1.0 N J:CYS85 4.8 77.6 1.0 N J:LYS87 4.8 88.9 1.0 CA J:CYS88 4.9 75.0 1.0 CA J:LYS74 4.9 72.3 1.0

Zinc binding site 2 out of 2 in the Cryo-Em Structure of Escherichia Coli Rna Polymerase Bound to T7A1 Promoter Dna


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Cryo-Em Structure of Escherichia Coli Rna Polymerase Bound to T7A1 Promoter Dna within 5.0Å range: probe atom residue distance (Å) B Occ J:Zn1503 b:64.7 occ:1.00 SG J:CYS898 2.3 41.4 1.0 SG J:CYS814 2.3 55.3 1.0 SG J:CYS888 2.3 49.7 1.0 CB J:CYS895 2.4 38.0 1.0 CB J:CYS898 3.0 41.4 1.0 SG J:CYS895 3.0 38.0 1.0 CB J:CYS814 3.3 55.3 1.0 CA J:CYS895 3.7 38.0 1.0 CB J:CYS888 3.8 49.7 1.0 N J:CYS895 3.8 38.0 1.0 NH2 J:ARG883 3.9 51.6 1.0 N J:CYS814 4.1 55.3 1.0 N J:CYS898 4.1 41.4 1.0 CA J:CYS898 4.1 41.4 1.0 OG1 J:THR816 4.1 61.6 1.0 CA J:CYS888 4.2 49.7 1.0 OG1 J:THR890 4.2 48.3 1.0 CA J:CYS814 4.3 55.3 1.0 C J:CYS895 4.4 38.0 1.0 N J:ASP889 4.5 55.8 1.0 O J:CYS895 4.7 38.0 1.0 C J:CYS888 4.8 49.7 1.0 CZ J:ARG883 4.9 51.6 1.0 N J:THR890 5.0 48.3 1.0

R.M.Saecker, J.Chen, C.Chiu, B.Malone, J.Sotiris, M.Ebrahim, L.Y.Yen, E.T.Eng, S.A.Darst. Structural Origins of Escherichia Coli Rna Polymerase Open Promoter Complex Stability To Be Published.