Zinc in PDB 7kze: Substrate-Dependent Divergence of Leukotriene A4 Hydrolase Aminopeptidase Activity

All present enzymatic activity of Substrate-Dependent Divergence of Leukotriene A4 Hydrolase Aminopeptidase Activity:
3.3.2.6; 3.4.11.4;

The structure of Substrate-Dependent Divergence of Leukotriene A4 Hydrolase Aminopeptidase Activity, PDB code: 7kze was solved by K.H.Lee, Y.Shim, M.Paige, S.M.Noble, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	53.84 / 2.90
Space group	P 32
Cell size a, b, c (Å), α, β, γ (°)	139.888, 139.888, 84.343, 90, 90, 120
R / Rfree (%)	19.5 / 20.9

The binding sites of Zinc atom in the Substrate-Dependent Divergence of Leukotriene A4 Hydrolase Aminopeptidase Activity (pdb code 7kze). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 3 binding sites of Zinc where determined in the Substrate-Dependent Divergence of Leukotriene A4 Hydrolase Aminopeptidase Activity, PDB code: 7kze:
Jump to Zinc binding site number: 1; 2; 3;

Zinc binding site 1 out of 3 in the Substrate-Dependent Divergence of Leukotriene A4 Hydrolase Aminopeptidase Activity


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Substrate-Dependent Divergence of Leukotriene A4 Hydrolase Aminopeptidase Activity within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn701 b:17.7 occ:1.00 OE1 A:GLU318 1.9 15.7 1.0 NE2 A:HIS295 2.2 15.6 1.0 NE2 A:HIS299 2.3 15.2 1.0 O A:HOH801 2.5 17.6 1.0 CD A:GLU318 2.6 14.8 1.0 OE2 A:GLU318 2.7 15.3 1.0 CD2 A:HIS295 2.9 14.1 1.0 O A:HOH860 3.1 17.6 1.0 CD2 A:HIS299 3.1 14.7 1.0 CE1 A:HIS295 3.3 13.9 1.0 CE1 A:HIS299 3.3 14.3 1.0 CE2 A:TYR383 3.6 16.6 1.0 OH A:TYR383 3.7 17.4 1.0 OE1 A:GLU271 3.7 17.5 1.0 O A:HOH956 3.9 17.4 1.0 CG A:GLU318 4.0 15.2 1.0 CZ A:TYR383 4.1 17.1 1.0 O A:HOH807 4.1 17.8 1.0 CG A:HIS295 4.2 14.5 1.0 O A:HOH994 4.2 19.7 1.0 ND1 A:HIS295 4.3 14.7 1.0 CG A:HIS299 4.3 14.3 1.0 ND1 A:HIS299 4.4 13.9 1.0 OE2 A:GLU296 4.4 16.7 1.0 CG2 A:THR321 4.6 14.0 1.0 CD2 A:TYR383 4.6 16.6 1.0 CD A:GLU271 4.6 16.3 1.0 CB A:GLU318 4.7 14.6 1.0 O A:HOH910 4.7 17.5 1.0 OE1 A:GLU296 4.8 15.8 1.0 CA A:GLU318 4.9 14.9 1.0 OE2 A:GLU271 4.9 16.4 1.0 CD A:GLU296 4.9 16.3 1.0 CB A:THR321 5.0 14.2 1.0

Zinc binding site 2 out of 3 in the Substrate-Dependent Divergence of Leukotriene A4 Hydrolase Aminopeptidase Activity


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Substrate-Dependent Divergence of Leukotriene A4 Hydrolase Aminopeptidase Activity within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn701 b:27.3 occ:1.00 OE1 B:GLU318 1.9 23.1 1.0 NE2 B:HIS299 2.2 22.6 1.0 NE2 B:HIS295 2.3 22.2 1.0 OE2 B:GLU318 2.5 24.2 1.0 CD B:GLU318 2.5 23.3 1.0 O B:HOH895 3.0 21.6 1.0 CD2 B:HIS299 3.1 22.1 1.0 CD2 B:HIS295 3.1 21.4 1.0 CE1 B:HIS299 3.3 22.3 1.0 CE1 B:HIS295 3.4 21.8 1.0 O B:HOH821 3.4 26.3 1.0 OH B:TYR383 3.7 24.0 1.0 CE2 B:TYR383 3.7 24.2 1.0 OE1 B:GLU271 3.9 26.0 1.0 CG B:GLU318 4.0 23.1 1.0 CZ B:TYR383 4.1 24.2 1.0 CG B:HIS299 4.3 21.6 1.0 ND1 B:HIS299 4.3 21.4 1.0 CG B:HIS295 4.3 21.7 1.0 ND1 B:HIS295 4.4 22.1 1.0 OE2 B:GLU271 4.4 25.1 1.0 CD B:GLU271 4.4 25.6 1.0 OE1 B:GLU296 4.6 24.2 1.0 CB B:GLU318 4.7 22.9 1.0 CG2 B:THR321 4.8 21.3 1.0 CD2 B:TYR383 4.8 24.7 1.0 O B:HOH856 4.9 23.6 1.0 OE2 B:GLU296 4.9 23.4 1.0 CA B:GLU318 4.9 22.7 1.0

Zinc binding site 3 out of 3 in the Substrate-Dependent Divergence of Leukotriene A4 Hydrolase Aminopeptidase Activity


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Substrate-Dependent Divergence of Leukotriene A4 Hydrolase Aminopeptidase Activity within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn701 b:21.9 occ:1.00 OE1 C:GLU318 1.9 18.5 1.0 NE2 C:HIS299 2.1 18.4 1.0 NE2 C:HIS295 2.1 18.6 1.0 O C:HOH876 2.4 18.4 1.0 CD C:GLU318 2.6 17.4 1.0 OE2 C:GLU318 2.7 17.7 1.0 CD2 C:HIS299 2.9 18.1 1.0 CD2 C:HIS295 3.0 18.2 1.0 CE1 C:HIS295 3.2 17.8 1.0 CE1 C:HIS299 3.2 17.4 1.0 O C:HOH848 3.6 20.0 1.0 CE2 C:TYR383 3.7 18.4 1.0 OH C:TYR383 3.8 18.7 1.0 OE1 C:GLU271 3.9 18.8 1.0 CG C:GLU318 4.1 17.9 1.0 CG C:HIS299 4.1 17.2 1.0 CZ C:TYR383 4.1 18.1 1.0 O C:HOH911 4.1 19.7 1.0 CG C:HIS295 4.2 18.3 1.0 ND1 C:HIS299 4.2 17.9 1.0 ND1 C:HIS295 4.2 18.2 1.0 CD C:GLU271 4.4 18.4 1.0 CG2 C:THR321 4.5 18.2 1.0 OE2 C:GLU271 4.5 18.5 1.0 OE2 C:GLU296 4.6 19.5 1.0 CB C:GLU318 4.7 18.8 1.0 CD2 C:TYR383 4.7 18.6 1.0 O C:HOH874 4.8 18.3 1.0 CA C:GLU318 4.8 19.1 1.0 OE1 C:GLU296 4.8 18.7 1.0 CB C:THR321 4.9 18.6 1.0

K.H.Lee, N.F.Ali, S.H.Lee, Z.Zhang, M.Burdick, Z.J.Beaulac, G.Petruncio, L.Li, J.Xiang, E.M.Chung, K.W.Foreman, S.M.Noble, Y.M.Shim, M.Paige. Substrate-Dependent Modulation of the Leukotriene A 4 Hydrolase Aminopeptidase Activity and Effect in A Murine Model of Acute Lung Inflammation. Sci Rep V. 12 9443 2022.
ISSN: ESSN 2045-2322
PubMed: 35676292
DOI: 10.1038/S41598-022-13238-6