Zinc in PDB 7jpe: Room Temperature Structure of Sars-Cov-2 NSP10/NSP16 Methyltransferase in A Complex with M7GPPPA Cap-0 and Sam Determined By Fixed-Target Serial Crystallography

The structure of Room Temperature Structure of Sars-Cov-2 NSP10/NSP16 Methyltransferase in A Complex with M7GPPPA Cap-0 and Sam Determined By Fixed-Target Serial Crystallography, PDB code: 7jpe was solved by M.Wilamowski, D.A.Sherrell, G.Minasov, Y.Kim, L.Shuvalova, A.Lavens, R.Chard, M.Rosas-Lemus, N.Maltseva, R.Jedrzejczak, K.Michalska, K.J.F.Satchell, A.Joachimiak, Center For Structural Genomics Ofinfectious Diseases (Csgid), with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	49.72 / 2.18
Space group	P 31 2 1
Cell size a, b, c (Å), α, β, γ (°)	170.930, 170.930, 52.786, 90.00, 90.00, 120.00
R / Rfree (%)	21.7 / 23.7

The binding sites of Zinc atom in the Room Temperature Structure of Sars-Cov-2 NSP10/NSP16 Methyltransferase in A Complex with M7GPPPA Cap-0 and Sam Determined By Fixed-Target Serial Crystallography (pdb code 7jpe). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Room Temperature Structure of Sars-Cov-2 NSP10/NSP16 Methyltransferase in A Complex with M7GPPPA Cap-0 and Sam Determined By Fixed-Target Serial Crystallography, PDB code: 7jpe:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Room Temperature Structure of Sars-Cov-2 NSP10/NSP16 Methyltransferase in A Complex with M7GPPPA Cap-0 and Sam Determined By Fixed-Target Serial Crystallography


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Room Temperature Structure of Sars-Cov-2 NSP10/NSP16 Methyltransferase in A Complex with M7GPPPA Cap-0 and Sam Determined By Fixed-Target Serial Crystallography within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn201 b:40.8 occ:1.00 SG B:CYS90 2.1 47.0 1.0 NE2 B:HIS83 2.2 61.0 1.0 SG B:CYS74 2.2 34.5 1.0 SG B:CYS77 2.4 35.3 1.0 CD2 B:HIS83 3.1 58.6 1.0 CB B:CYS74 3.1 34.1 1.0 CE1 B:HIS83 3.2 60.6 1.0 CB B:CYS90 3.2 51.9 1.0 CB B:CYS77 3.5 35.0 1.0 N B:CYS77 3.7 37.6 1.0 CA B:CYS77 4.1 36.5 1.0 CG B:HIS83 4.2 61.8 1.0 ND1 B:HIS83 4.3 61.4 1.0 CA B:CYS90 4.3 55.6 1.0 CA B:CYS74 4.5 34.9 1.0 CB B:TYR76 4.6 42.6 1.0 C B:TYR76 4.6 39.3 1.0 N B:LYS93 4.9 40.6 1.0 C B:CYS74 5.0 36.2 1.0 CB B:LYS93 5.0 42.7 1.0 CA B:TYR76 5.0 41.1 1.0

Zinc binding site 2 out of 2 in the Room Temperature Structure of Sars-Cov-2 NSP10/NSP16 Methyltransferase in A Complex with M7GPPPA Cap-0 and Sam Determined By Fixed-Target Serial Crystallography


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Room Temperature Structure of Sars-Cov-2 NSP10/NSP16 Methyltransferase in A Complex with M7GPPPA Cap-0 and Sam Determined By Fixed-Target Serial Crystallography within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn202 b:77.9 occ:1.00 SG B:CYS120 2.2 79.6 1.0 SG B:CYS117 2.3 73.4 1.0 SG B:CYS128 2.5 73.1 1.0 SG B:CYS130 2.5 0.5 1.0 CB B:CYS117 3.1 73.9 1.0 CB B:CYS128 3.5 76.0 1.0 CB B:CYS130 3.5 0.2 1.0 CB B:CYS120 3.6 76.1 1.0 N B:CYS120 3.8 74.3 1.0 CA B:CYS128 4.0 77.0 1.0 N B:CYS130 4.1 0.1 1.0 CA B:CYS120 4.2 73.0 1.0 N B:SER129 4.3 87.5 1.0 CA B:CYS130 4.4 0.6 1.0 CA B:CYS117 4.5 73.7 1.0 C B:CYS128 4.6 80.8 1.0 CB B:VAL119 4.6 83.3 1.0 C B:VAL119 4.9 75.3 1.0 N B:GLY121 5.0 65.6 1.0 C B:CYS120 5.0 67.3 1.0 C B:CYS117 5.0 75.6 1.0

M.Wilamowski, D.A.Sherrell, G.Minasov, Y.Kim, L.Shuvalova, A.Lavens, R.Chard, M.Rosas-Lemus, N.Maltseva, R.Jedrzejczak, K.Michalska, K.J.F.Satchell, A.Joachimiak, Center For Structural Genomics Of Infectious Diseases(Csgid). Room Temperature Structure of Sars-Cov-2 NSP10/NSP16 Methyltransferase in A Complex with M7GPPPA Cap-0 and Sam Determined By Fixed-Target Serial Crystallography To Be Published.