Zinc in PDB 7jib: Room Temperature Crystal Structure of NSP10/NSP16 From Sars-Cov-2 with Substrates and Products of 2'-O-Methylation of the Cap-1

The structure of Room Temperature Crystal Structure of NSP10/NSP16 From Sars-Cov-2 with Substrates and Products of 2'-O-Methylation of the Cap-1, PDB code: 7jib was solved by M.Wilamowski, G.Minasov, Y.Kim, D.A.Sherrell, L.Shuvalova, A.Lavens, R.Chard, M.Rosas-Lemus, N.Maltseva, R.Jedrzejczak, K.Michalska, K.J.F.Satchell, A.Joachimiak, Center For Structural Genomics Ofinfectious Diseases (Csgid), with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.59 / 2.65
Space group	P 31 2 1
Cell size a, b, c (Å), α, β, γ (°)	170.830, 170.830, 52.738, 90.00, 90.00, 120.00
R / Rfree (%)	15.5 / 18.1

The structure of Room Temperature Crystal Structure of NSP10/NSP16 From Sars-Cov-2 with Substrates and Products of 2'-O-Methylation of the Cap-1 also contains other interesting chemical elements:

Chlorine

(Cl)

2 atoms

The binding sites of Zinc atom in the Room Temperature Crystal Structure of NSP10/NSP16 From Sars-Cov-2 with Substrates and Products of 2'-O-Methylation of the Cap-1 (pdb code 7jib). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Room Temperature Crystal Structure of NSP10/NSP16 From Sars-Cov-2 with Substrates and Products of 2'-O-Methylation of the Cap-1, PDB code: 7jib:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Room Temperature Crystal Structure of NSP10/NSP16 From Sars-Cov-2 with Substrates and Products of 2'-O-Methylation of the Cap-1


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Room Temperature Crystal Structure of NSP10/NSP16 From Sars-Cov-2 with Substrates and Products of 2'-O-Methylation of the Cap-1 within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn4401 b:68.3 occ:1.00 NE2 B:HIS83 2.1 95.5 1.0 SG B:CYS90 2.3 82.4 1.0 SG B:CYS74 2.3 64.4 1.0 SG B:CYS77 2.3 70.9 1.0 CD2 B:HIS83 3.1 95.4 1.0 CE1 B:HIS83 3.1 93.7 1.0 CB B:CYS74 3.2 67.7 1.0 CB B:CYS90 3.3 90.6 1.0 CB B:CYS77 3.4 66.8 1.0 N B:CYS77 3.6 69.8 1.0 CA B:CYS77 4.0 64.8 1.0 ND1 B:HIS83 4.2 0.0 1.0 CG B:HIS83 4.2 0.7 1.0 CA B:CYS90 4.4 97.9 1.0 CB B:TYR76 4.4 79.3 1.0 C B:TYR76 4.5 72.0 1.0 CA B:CYS74 4.6 65.5 1.0 CA B:TYR76 4.9 73.8 1.0 N B:LYS93 4.9 61.1 1.0 O B:CYS74 5.0 66.6 1.0 C B:CYS74 5.0 65.6 1.0

Zinc binding site 2 out of 2 in the Room Temperature Crystal Structure of NSP10/NSP16 From Sars-Cov-2 with Substrates and Products of 2'-O-Methylation of the Cap-1


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Room Temperature Crystal Structure of NSP10/NSP16 From Sars-Cov-2 with Substrates and Products of 2'-O-Methylation of the Cap-1 within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn4402 b:99.6 occ:1.00 SG B:CYS128 2.4 1.0 1.0 SG B:CYS120 2.4 0.7 1.0 SG B:CYS130 2.4 0.1 1.0 SG B:CYS117 2.4 0.2 1.0 CB B:CYS117 3.3 0.2 1.0 CB B:CYS130 3.4 0.9 1.0 CB B:CYS128 3.5 0.9 1.0 CB B:CYS120 3.5 1.0 1.0 N B:CYS120 3.7 0.4 1.0 O B:HOH4517 3.8 79.9 1.0 CA B:CYS128 4.0 0.3 1.0 CA B:CYS120 4.2 0.8 1.0 N B:CYS130 4.2 0.7 1.0 CB B:VAL119 4.4 0.6 1.0 C B:CYS128 4.4 0.1 1.0 CA B:CYS130 4.4 0.9 1.0 N B:SER129 4.5 0.9 1.0 CA B:CYS117 4.8 0.7 1.0 C B:VAL119 4.8 0.5 1.0 CG1 B:VAL119 4.9 0.4 1.0 CA B:VAL119 5.0 0.5 1.0 N B:VAL119 5.0 0.2 1.0

M.Wilamowski, G.Minasov, Y.Kim, D.A.Sherrell, L.Shuvalova, A.Lavens, R.Chard, M.Rosas-Lemus, N.Maltseva, R.Jedrzejczak, K.Michalska, K.J.F.Satchell, A.Joachimiak, Center For Structural Genomics Of Infectious Diseases(Csgid). Room Temperature Crystal Structure of NSP10/NSP16 From Sars-Cov-2 with Substrates and Products of 2'-O-Methylation of the Cap-1 To Be Published.