Zinc in PDB 7dnr: Crystal Structure of Zn-Bound Sis Domain of Glucosamine-6-P Synthase From E. Coli

All present enzymatic activity of Crystal Structure of Zn-Bound Sis Domain of Glucosamine-6-P Synthase From E. Coli:
2.6.1.16;

The structure of Crystal Structure of Zn-Bound Sis Domain of Glucosamine-6-P Synthase From E. Coli, PDB code: 7dnr was solved by C.Gao, J.Xiao, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	23.52 / 1.70
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	70.099, 67.889, 73.952, 90, 111.44, 90
R / Rfree (%)	21.5 / 23.5

The binding sites of Zinc atom in the Crystal Structure of Zn-Bound Sis Domain of Glucosamine-6-P Synthase From E. Coli (pdb code 7dnr). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 3 binding sites of Zinc where determined in the Crystal Structure of Zn-Bound Sis Domain of Glucosamine-6-P Synthase From E. Coli, PDB code: 7dnr:
Jump to Zinc binding site number: 1; 2; 3;

Zinc binding site 1 out of 3 in the Crystal Structure of Zn-Bound Sis Domain of Glucosamine-6-P Synthase From E. Coli


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Zn-Bound Sis Domain of Glucosamine-6-P Synthase From E. Coli within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn701 b:20.6 occ:1.00 O A:HOH910 2.1 21.2 1.0 NE2 A:HIS505 2.2 39.1 1.0 SG B:CYS301 2.3 21.9 1.0 O A:HOH999 2.6 32.8 1.0 CE1 A:HIS505 3.0 39.2 1.0 CB B:CYS301 3.1 21.8 1.0 CD2 A:HIS505 3.3 39.2 1.0 O B:HOH942 4.1 23.2 1.0 OE1 A:GLU502 4.2 30.4 1.0 ND1 A:HIS505 4.2 39.4 1.0 CA B:CYS301 4.4 21.7 1.0 CG A:HIS505 4.4 39.5 1.0 C B:CYS301 4.4 21.9 1.0 CG2 B:THR353 4.5 24.1 1.0 O A:HOH824 4.5 28.2 1.0 N B:GLY302 4.6 20.4 1.0 CD A:LYS504 4.6 48.9 1.0 O B:HOH1026 4.7 34.2 1.0 CG A:LYS504 4.7 48.8 1.0 O B:HOH1004 4.9 35.1 1.0 O B:CYS301 4.9 21.7 1.0

Zinc binding site 2 out of 3 in the Crystal Structure of Zn-Bound Sis Domain of Glucosamine-6-P Synthase From E. Coli


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of Zn-Bound Sis Domain of Glucosamine-6-P Synthase From E. Coli within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn702 b:16.4 occ:1.00 O A:HOH1001 2.2 37.5 1.0 NE2 B:HIS467 2.2 24.6 1.0 NE2 A:HIS467 2.2 24.6 1.0 NE2 A:HIS466 2.2 26.4 1.0 CD2 A:HIS467 2.8 24.7 1.0 CE1 A:HIS466 2.9 26.6 1.0 CE1 B:HIS467 3.1 25.0 1.0 CD2 B:HIS467 3.2 24.5 1.0 CD2 A:HIS466 3.2 26.5 1.0 CE1 A:HIS467 3.4 24.6 1.0 ND1 A:HIS466 4.0 26.7 1.0 CG A:HIS467 4.1 24.7 1.0 CG A:HIS466 4.2 26.7 1.0 ND1 B:HIS467 4.3 24.8 1.0 CG B:HIS467 4.3 24.5 1.0 ND1 A:HIS467 4.3 24.5 1.0 CD2 B:HIS494 4.6 28.1 1.0 CB B:HIS466 4.8 27.6 1.0 NE2 B:HIS494 5.0 28.0 1.0

Zinc binding site 3 out of 3 in the Crystal Structure of Zn-Bound Sis Domain of Glucosamine-6-P Synthase From E. Coli


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Crystal Structure of Zn-Bound Sis Domain of Glucosamine-6-P Synthase From E. Coli within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn701 b:20.8 occ:1.00 O B:HOH977 2.2 38.1 1.0 NE2 B:HIS505 2.2 44.4 1.0 SG A:CYS301 2.3 23.1 1.0 O B:HOH1019 2.4 29.1 1.0 CE1 B:HIS505 3.0 45.0 1.0 CB A:CYS301 3.2 22.8 1.0 CD2 B:HIS505 3.3 44.3 1.0 O A:HOH970 3.9 31.2 1.0 OE1 B:GLU502 4.0 27.6 1.0 ND1 B:HIS505 4.2 45.0 1.0 CG B:HIS505 4.4 44.7 1.0 C A:CYS301 4.4 23.3 1.0 CG2 A:THR353 4.4 23.1 1.0 CA A:CYS301 4.4 22.8 1.0 O A:HOH961 4.5 24.2 1.0 N A:GLY302 4.5 19.8 1.0 O B:HOH843 4.6 37.7 1.0 O A:HOH990 4.7 38.1 1.0 O A:CYS301 4.8 23.5 1.0 CD B:GLU502 4.9 27.3 1.0

X.Zeng, T.Wei, Y.Liu, X.Wang, T.Feng, Y.Cheng, W.Qin, C.Gao, J.Xiao, C.Wang. Discovery of Metal-Binding Proteins By Thermal Proteome Profiling To Be Published.