Zinc in PDB 7c97: Cryo-Em Structure of An Escherichia Coli Rnap-Promoter Open Complex (Rpo) with Sspa

All present enzymatic activity of Cryo-Em Structure of An Escherichia Coli Rnap-Promoter Open Complex (Rpo) with Sspa:
2.5.1.18; 2.7.7.6;

The structure of Cryo-Em Structure of An Escherichia Coli Rnap-Promoter Open Complex (Rpo) with Sspa also contains other interesting chemical elements:

Magnesium

(Mg)

1 atom

The binding sites of Zinc atom in the Cryo-Em Structure of An Escherichia Coli Rnap-Promoter Open Complex (Rpo) with Sspa (pdb code 7c97). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Cryo-Em Structure of An Escherichia Coli Rnap-Promoter Open Complex (Rpo) with Sspa, PDB code: 7c97:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Cryo-Em Structure of An Escherichia Coli Rnap-Promoter Open Complex (Rpo) with Sspa


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Cryo-Em Structure of An Escherichia Coli Rnap-Promoter Open Complex (Rpo) with Sspa within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn2002 b:75.5 occ:1.00 SG D:CYS70 2.4 14.0 1.0 SG D:CYS88 2.4 24.5 1.0 SG D:CYS85 2.4 19.5 1.0 SG D:CYS72 2.4 19.0 1.0 CB D:CYS72 3.0 19.0 1.0 N D:CYS88 3.4 24.5 1.0 N D:CYS72 3.5 19.0 1.0 CB D:CYS85 3.5 19.5 1.0 CB D:LYS87 3.6 30.8 1.0 O D:CYS88 3.7 24.5 1.0 CA D:CYS72 3.8 19.0 1.0 CB D:CYS88 3.9 24.5 1.0 CB D:CYS70 4.0 14.0 1.0 CA D:CYS88 4.0 24.5 1.0 C D:CYS88 4.3 24.5 1.0 C D:LYS87 4.3 30.8 1.0 CA D:LYS87 4.4 30.8 1.0 N D:LEU71 4.4 13.6 1.0 N D:LYS87 4.5 30.8 1.0 N D:GLY73 4.6 15.7 1.0 C D:LEU71 4.6 13.6 1.0 CG D:LYS87 4.7 30.8 1.0 C D:CYS72 4.8 19.0 1.0 CA D:CYS85 4.9 19.5 1.0 CA D:LEU71 4.9 13.6 1.0

Zinc binding site 2 out of 2 in the Cryo-Em Structure of An Escherichia Coli Rnap-Promoter Open Complex (Rpo) with Sspa


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Cryo-Em Structure of An Escherichia Coli Rnap-Promoter Open Complex (Rpo) with Sspa within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn2003 b:47.6 occ:1.00 CB D:CYS814 2.3 28.4 1.0 SG D:CYS898 2.3 10.2 1.0 SG D:CYS888 2.3 18.4 1.0 SG D:CYS895 2.4 12.9 1.0 SG D:CYS814 3.1 28.4 1.0 NH2 D:ARG883 3.2 19.9 1.0 CB D:CYS888 3.3 18.4 1.0 CB D:CYS898 3.3 10.2 1.0 CB D:CYS895 3.5 12.9 1.0 CA D:CYS814 3.6 28.4 1.0 CA D:CYS888 3.8 18.4 1.0 N D:CYS814 3.8 28.4 1.0 N D:ASP889 4.1 23.3 1.0 OG1 D:THR890 4.3 21.8 1.0 N D:CYS895 4.4 12.9 1.0 CZ D:ARG883 4.4 19.9 1.0 CA D:CYS898 4.4 10.2 1.0 N D:CYS898 4.5 10.2 1.0 C D:CYS814 4.5 28.4 1.0 C D:CYS888 4.5 18.4 1.0 CA D:CYS895 4.5 12.9 1.0 N D:GLY815 4.6 33.5 1.0

L.Wei, F.Yu. Structural Basis For Transcription Inhibition By E. Coli Sspa Nucleic Acids Res. 2020.
ISSN: ESSN 1362-4962