Zinc in PDB 7alc: Human Gch-Gfrp Stimulatory Complex

All present enzymatic activity of Human Gch-Gfrp Stimulatory Complex:
3.5.4.16;

The structure of Human Gch-Gfrp Stimulatory Complex, PDB code: 7alc was solved by R.Ebenhoch, H.Nar, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	86.99 / 1.73
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	163.853, 113.922, 113.91, 90, 130.21, 90
R / Rfree (%)	19.7 / 22.7

The binding sites of Zinc atom in the Human Gch-Gfrp Stimulatory Complex (pdb code 7alc). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 5 binding sites of Zinc where determined in the Human Gch-Gfrp Stimulatory Complex, PDB code: 7alc:
Jump to Zinc binding site number: 1; 2; 3; 4; 5;

Zinc binding site 1 out of 5 in the Human Gch-Gfrp Stimulatory Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Human Gch-Gfrp Stimulatory Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn301 b:29.9 occ:1.00 ND1 A:HIS144 2.1 27.5 1.0 SG A:CYS141 2.1 39.7 1.0 SG A:CYS212 2.3 38.5 1.0 CE1 A:HIS144 3.1 28.4 1.0 CG A:HIS144 3.1 26.5 1.0 CB A:CYS141 3.3 26.3 1.0 CB A:CYS212 3.4 36.9 1.0 CB A:HIS144 3.5 24.8 1.0 N A:HIS144 3.9 25.2 1.0 O A:HOH448 4.2 28.9 1.0 NE2 A:HIS144 4.2 28.8 1.0 CD2 A:HIS144 4.3 27.6 1.0 CA A:HIS144 4.3 24.4 1.0 CB A:HIS143 4.3 26.3 1.0 O A:HOH534 4.6 49.8 1.0 O A:HOH523 4.7 48.8 1.0 CA A:CYS141 4.7 22.8 1.0 C A:HIS143 4.7 26.1 1.0 CA A:CYS212 4.8 36.5 1.0 CA A:HIS143 4.9 25.3 1.0 N A:HIS143 5.0 24.6 1.0

Zinc binding site 2 out of 5 in the Human Gch-Gfrp Stimulatory Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Human Gch-Gfrp Stimulatory Complex within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn301 b:37.7 occ:1.00 ND1 B:HIS144 2.1 36.0 1.0 SG B:CYS212 2.2 39.1 1.0 SG B:CYS141 2.3 36.5 1.0 O B:HOH471 2.5 49.5 1.0 CE1 B:HIS144 3.1 36.7 1.0 CG B:HIS144 3.1 34.2 1.0 CB B:CYS212 3.3 36.8 1.0 CB B:CYS141 3.4 27.9 1.0 CB B:HIS144 3.5 31.1 1.0 N B:HIS144 3.9 28.9 1.0 O B:HOH438 4.1 29.9 1.0 NE2 B:HIS144 4.2 37.2 1.0 CD2 B:HIS144 4.3 35.9 1.0 CA B:HIS144 4.3 29.3 1.0 CB B:HIS143 4.4 29.8 1.0 O C:HOH468 4.6 56.6 1.0 CA B:CYS212 4.7 36.7 1.0 C B:HIS143 4.7 29.6 1.0 CA B:CYS141 4.8 25.1 1.0 CA B:HIS143 4.9 28.9 1.0 N B:HIS143 4.9 27.1 1.0

Zinc binding site 3 out of 5 in the Human Gch-Gfrp Stimulatory Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Human Gch-Gfrp Stimulatory Complex within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn301 b:33.3 occ:1.00 ND1 C:HIS144 1.9 25.5 1.0 SG C:CYS212 2.2 44.0 1.0 SG C:CYS141 2.4 32.2 1.0 CE1 C:HIS144 2.8 26.4 1.0 CG C:HIS144 3.0 23.0 1.0 CB C:CYS212 3.4 41.8 1.0 CB C:CYS141 3.4 23.8 1.0 CB C:HIS144 3.5 21.1 1.0 N C:HIS144 3.9 21.9 1.0 NE2 C:HIS144 4.0 26.7 1.0 CD2 C:HIS144 4.1 24.5 1.0 CB C:HIS143 4.2 21.5 1.0 CA C:HIS144 4.3 21.1 1.0 O C:HOH428 4.5 33.8 1.0 C C:HIS143 4.7 23.0 1.0 CA C:CYS212 4.8 40.5 1.0 CA C:CYS141 4.8 21.1 1.0 CA C:HIS143 4.9 21.6 1.0 N C:HIS143 5.0 21.2 1.0

Zinc binding site 4 out of 5 in the Human Gch-Gfrp Stimulatory Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Human Gch-Gfrp Stimulatory Complex within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn301 b:33.9 occ:1.00 ND1 D:HIS144 2.1 31.9 1.0 SG D:CYS212 2.2 39.7 1.0 SG D:CYS141 2.3 38.6 1.0 CE1 D:HIS144 3.0 32.2 1.0 CG D:HIS144 3.1 30.9 1.0 CB D:CYS212 3.3 37.9 1.0 CB D:CYS141 3.4 28.2 1.0 CB D:HIS144 3.5 28.9 1.0 N D:HIS144 3.9 28.5 1.0 O D:HOH466 4.0 32.8 1.0 NE2 D:HIS144 4.2 33.0 1.0 O D:HOH525 4.2 63.6 1.0 CD2 D:HIS144 4.2 32.3 1.0 CA D:HIS144 4.3 28.4 1.0 CB D:HIS143 4.4 31.4 1.0 CA D:CYS212 4.7 37.9 1.0 C D:HIS143 4.7 29.5 1.0 CA D:CYS141 4.8 25.9 1.0 CA D:HIS143 4.9 29.5 1.0 N D:HIS143 5.0 28.3 1.0

Zinc binding site 5 out of 5 in the Human Gch-Gfrp Stimulatory Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 5 of Human Gch-Gfrp Stimulatory Complex within 5.0Å range: probe atom residue distance (Å) B Occ E:Zn301 b:28.8 occ:1.00 ND1 E:HIS144 2.0 24.6 1.0 SG E:CYS212 2.3 38.7 1.0 SG E:CYS141 2.3 29.9 1.0 CE1 E:HIS144 2.9 25.0 1.0 CG E:HIS144 3.0 22.4 1.0 CB E:CYS212 3.1 37.6 1.0 CB E:CYS141 3.4 22.5 1.0 CB E:HIS144 3.4 20.9 1.0 N E:HIS144 3.9 19.7 1.0 NE2 E:HIS144 4.1 24.9 1.0 CD2 E:HIS144 4.1 23.2 1.0 CB E:HIS143 4.3 18.8 1.0 O E:HOH489 4.3 57.6 1.0 CA E:HIS144 4.3 19.8 1.0 O E:HOH433 4.3 35.0 1.0 O E:HOH449 4.4 21.6 1.0 CA E:CYS212 4.6 37.6 1.0 C E:HIS143 4.6 19.5 1.0 CA E:HIS143 4.8 17.8 1.0 CA E:CYS141 4.8 20.4 1.0 N E:HIS143 4.9 16.3 1.0

R.Ebenhoch, M.Bauer, D.Reinert, A.Kersting, S.Huber, A.Schmid, I.Hinz, M.Feiler, K.Mueller, H.Nar. Using Hybrid Approaches to Study the Allosteric Regulation of Gtp Cyclohydrolase I To Be Published.