Zinc in PDB 7ago: Crystal Structure of the N-Acetylmuramyl-L-Alanine Amidase, AMI1, From Mycobacterium Abscessus Bound to L-Alanine-D-Isoglutamine

The structure of Crystal Structure of the N-Acetylmuramyl-L-Alanine Amidase, AMI1, From Mycobacterium Abscessus Bound to L-Alanine-D-Isoglutamine, PDB code: 7ago was solved by M.Blaise, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	47.30 / 1.70
Space group	P 41 21 2
Cell size a, b, c (Å), α, β, γ (°)	85.740, 85.740, 75.630, 90.00, 90.00, 90.00
R / Rfree (%)	16.2 / 18.7

The binding sites of Zinc atom in the Crystal Structure of the N-Acetylmuramyl-L-Alanine Amidase, AMI1, From Mycobacterium Abscessus Bound to L-Alanine-D-Isoglutamine (pdb code 7ago). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total only one binding site of Zinc was determined in the Crystal Structure of the N-Acetylmuramyl-L-Alanine Amidase, AMI1, From Mycobacterium Abscessus Bound to L-Alanine-D-Isoglutamine, PDB code: 7ago:

Zinc binding site 1 out of 1 in the Crystal Structure of the N-Acetylmuramyl-L-Alanine Amidase, AMI1, From Mycobacterium Abscessus Bound to L-Alanine-D-Isoglutamine


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of the N-Acetylmuramyl-L-Alanine Amidase, AMI1, From Mycobacterium Abscessus Bound to L-Alanine-D-Isoglutamine within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn303 b:13.0 occ:0.77 OE1 A:GLU60 2.0 18.0 1.0 NE2 A:HIS25 2.1 16.9 1.0 O A:HOH433 2.1 18.0 1.0 ND1 A:HIS115 2.1 16.0 1.0 CD A:GLU60 2.8 17.2 1.0 OE2 A:GLU60 3.0 15.6 1.0 CE1 A:HIS115 3.0 18.5 1.0 CD2 A:HIS25 3.0 16.2 1.0 CE1 A:HIS25 3.1 14.3 1.0 CG A:HIS115 3.2 15.0 1.0 CB A:HIS115 3.5 15.2 1.0 O A:ALA116 3.9 16.9 1.0 OE2 A:GLU190 4.1 21.2 1.0 NE2 A:HIS115 4.2 14.0 1.0 ND1 A:HIS25 4.2 16.7 1.0 CG A:HIS25 4.2 16.4 1.0 CG A:GLU60 4.2 19.1 1.0 CD2 A:HIS115 4.2 15.7 1.0 CA A:ALA302 4.3 44.9 0.9 CA A:HIS115 4.4 14.9 1.0 N A:ALA116 4.4 15.8 1.0 N A:ZGL301 4.6 42.8 0.9 O A:ZGL301 4.7 37.6 0.9 CB A:ALA302 4.7 42.1 0.9 OE1 A:GLU190 4.8 19.3 1.0 CD A:GLU190 4.8 21.3 1.0 C A:ALA116 4.9 16.2 1.0 CA A:GLY51 4.9 16.6 1.0 C A:HIS115 4.9 17.5 1.0 C A:ALA302 5.0 44.8 0.9

T.Kussau, N.Van Wyk, M.D.Johansen, H.M.A.B.Alsarraf, A.Neyret, C.Hamela, K.K.Sorensen, M.B.Thygesen, C.Beauvineau, L.Kremer, M.Blaise. Functional Characterization of the N -Acetylmuramyl-L-Alanine Amidase, AMI1, From Mycobacterium Abscessus . Cells V. 9 2020.
ISSN: ESSN 2073-4409
PubMed: 33158165
DOI: 10.3390/CELLS9112410