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Zinc in PDB 7a4k: Trna-Guanine Transglycosylase C158S/C281S/Y330C/H333A Mutant in Complex with Tetramethylene Sulfoxide

Enzymatic activity of Trna-Guanine Transglycosylase C158S/C281S/Y330C/H333A Mutant in Complex with Tetramethylene Sulfoxide

All present enzymatic activity of Trna-Guanine Transglycosylase C158S/C281S/Y330C/H333A Mutant in Complex with Tetramethylene Sulfoxide:
2.4.2.29;

Protein crystallography data

The structure of Trna-Guanine Transglycosylase C158S/C281S/Y330C/H333A Mutant in Complex with Tetramethylene Sulfoxide, PDB code: 7a4k was solved by D.Nguyen, A.Heine, G.Klebe, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 42.81 / 1.68
Space group P 65 2 2
Cell size a, b, c (Å), α, β, γ (°) 128.27, 128.27, 114.971, 90, 90, 120
R / Rfree (%) 15.5 / 19

Zinc Binding Sites:

The binding sites of Zinc atom in the Trna-Guanine Transglycosylase C158S/C281S/Y330C/H333A Mutant in Complex with Tetramethylene Sulfoxide (pdb code 7a4k). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total only one binding site of Zinc was determined in the Trna-Guanine Transglycosylase C158S/C281S/Y330C/H333A Mutant in Complex with Tetramethylene Sulfoxide, PDB code: 7a4k:

Zinc binding site 1 out of 1 in 7a4k

Go back to Zinc Binding Sites List in 7a4k
Zinc binding site 1 out of 1 in the Trna-Guanine Transglycosylase C158S/C281S/Y330C/H333A Mutant in Complex with Tetramethylene Sulfoxide


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Trna-Guanine Transglycosylase C158S/C281S/Y330C/H333A Mutant in Complex with Tetramethylene Sulfoxide within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn401

b:22.6
occ:1.00
 ND1 A:HIS349 2.1 21.3 1.0
SG A:CYS320 2.3 22.1 1.0
SG A:CYS323 2.3 22.8 1.0
SG A:CYS318 2.3 22.4 1.0
CE1 A:HIS349 2.9 23.1 1.0
HE1 A:HIS349 3.0 27.7 1.0
HB2 A:CYS323 3.0 26.4 1.0
HB3 A:CYS318 3.0 26.2 1.0
HB3 A:CYS320 3.2 25.0 1.0
CG A:HIS349 3.2 21.0 1.0
CB A:CYS323 3.3 21.9 1.0
CB A:CYS318 3.3 21.9 1.0
H A:CYS323 3.3 28.0 1.0
HB2 A:HIS349 3.3 24.2 1.0
CB A:CYS320 3.4 20.8 1.0
H A:CYS320 3.4 27.9 1.0
HA A:HIS349 3.6 22.4 1.0
CB A:HIS349 3.7 20.1 1.0
HB2 A:CYS318 3.7 26.2 1.0
HB A:VAL322 3.9 27.6 1.0
N A:CYS323 3.9 23.3 1.0
HB3 A:CYS323 4.0 26.4 1.0
NE2 A:HIS349 4.1 21.9 1.0
N A:CYS320 4.1 23.3 1.0
HB2 A:CYS320 4.1 25.0 1.0
CA A:HIS349 4.2 18.7 1.0
CA A:CYS323 4.2 23.1 1.0
CA A:CYS320 4.2 21.5 1.0
CD2 A:HIS349 4.3 23.1 1.0
HB2 A:LEU314 4.3 27.0 1.0
O A:HIS349 4.5 20.9 1.0
HB3 A:ASP315 4.5 29.8 1.0
HE22 A:GLN356 4.5 33.5 1.0
HB3 A:ALA352 4.6 24.4 1.0
CA A:CYS318 4.6 21.8 1.0
HA A:CYS323 4.6 27.8 1.0
HD12 A:LEU314 4.6 27.9 1.0
HB3 A:HIS349 4.6 24.2 1.0
C A:CYS318 4.6 22.9 1.0
H A:ASP315 4.7 27.6 1.0
C A:CYS320 4.7 21.2 1.0
O A:CYS318 4.7 22.9 1.0
HB2 A:PHE353 4.7 26.5 1.0
C A:HIS349 4.7 20.6 1.0
O A:CYS320 4.8 21.6 1.0
H A:VAL322 4.8 29.2 1.0
CB A:VAL322 4.8 23.0 1.0
HE2 A:HIS349 4.8 26.3 1.0
C A:VAL322 4.9 24.1 1.0

Reference:

D.Nguyen, X.Xie, S.Jakobi, F.Terwesten, A.Metz, T.X.P.Nguyen, V.A.Palchykov, A.Heine, K.Reuter, G.Klebe. Targeting A Cryptic Pocket in A Protein-Protein Contact By Disulfide-Induced Rupture of A Homodimeric Interface Acs Chem.Biol. 2021.
ISSN: ESSN 1554-8937
DOI: 10.1021/ACSCHEMBIO.1C00296
Page generated: Tue Oct 29 16:19:26 2024

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