Zinc in PDB 6zus: Crystal Structure of the Effector ECP11-1 From Fulvia Fulva

The structure of Crystal Structure of the Effector ECP11-1 From Fulvia Fulva, PDB code: 6zus was solved by N.Lazar, C.Mesarich, Y.Petit-Houdenot, N.Talbi, I.Li De La Sierra-Gallay, E.Zelie, K.Blondeau, J.Gracy, B.Ollivier, A.Van De Wouw, M.H.Balesdent, A.Idnurm, H.Van Tilbeurgh, I.Fudal, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	45.55 / 1.62
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	40.64, 53.64, 86.15, 90, 90, 90
R / Rfree (%)	18.4 / 19.7

The binding sites of Zinc atom in the Crystal Structure of the Effector ECP11-1 From Fulvia Fulva (pdb code 6zus). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 4 binding sites of Zinc where determined in the Crystal Structure of the Effector ECP11-1 From Fulvia Fulva, PDB code: 6zus:
Jump to Zinc binding site number: 1; 2; 3; 4;

Zinc binding site 1 out of 4 in the Crystal Structure of the Effector ECP11-1 From Fulvia Fulva


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of the Effector ECP11-1 From Fulvia Fulva within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn206 b:21.3 occ:0.80 ND1 A:HIS12 2.0 20.9 1.0 ZN A:ZN207 2.2 29.6 0.5 O A:HOH399 2.3 15.3 1.0 CE1 A:HIS12 3.0 20.8 1.0 CG A:HIS12 3.1 19.4 1.0 CB A:HIS12 3.5 19.0 1.0 OH A:TYR124 3.9 21.5 1.0 NE2 A:HIS12 4.1 19.1 1.0 CE1 A:PHE14 4.1 21.3 1.0 CD2 A:HIS12 4.2 20.6 1.0 O A:HOH315 4.2 50.6 1.0 CE2 A:TYR124 4.4 18.9 1.0 CZ A:TYR124 4.5 18.6 1.0 CD1 A:PHE14 4.5 19.1 1.0 CH2 A:TRP10 4.7 20.3 1.0 CZ A:PHE14 4.7 20.7 1.0 SG A:CYS26 4.8 53.6 1.0 CA A:HIS12 5.0 18.1 1.0

Zinc binding site 2 out of 4 in the Crystal Structure of the Effector ECP11-1 From Fulvia Fulva


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of the Effector ECP11-1 From Fulvia Fulva within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn207 b:29.6 occ:0.50 ZN A:ZN206 2.2 21.3 0.8 O A:HOH315 3.0 50.6 1.0 CB A:CYS73 3.5 24.0 1.0 CB A:HIS12 3.6 19.0 1.0 ND1 A:HIS12 3.6 20.9 1.0 SG A:CYS26 3.6 53.6 1.0 O A:HOH399 3.7 15.3 1.0 CG A:HIS12 4.1 19.4 1.0 CZ3 A:TRP10 4.1 18.8 1.0 CD2 A:LEU31 4.1 22.6 1.0 CH2 A:TRP10 4.2 20.3 1.0 SG A:CYS73 4.4 27.8 1.0 CE1 A:PHE14 4.5 21.3 1.0 CB A:CYS26 4.5 37.9 1.0 O A:CYS73 4.8 18.8 1.0 CA A:CYS73 4.8 20.0 1.0 CE1 A:HIS12 4.8 20.8 1.0 CA A:HIS12 4.9 18.1 1.0 CG A:LEU31 5.0 22.5 1.0 O A:HOH313 5.0 43.0 1.0

Zinc binding site 3 out of 4 in the Crystal Structure of the Effector ECP11-1 From Fulvia Fulva


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Crystal Structure of the Effector ECP11-1 From Fulvia Fulva within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn208 b:25.5 occ:0.50 ZN A:ZN209 2.3 21.9 0.8 OXT A:GLN140 3.2 24.9 1.0 N A:GLY138 3.3 20.3 1.0 N A:MET0 3.3 27.9 1.0 ND1 A:HIS136 3.5 33.9 1.0 CA A:HIS-1 3.5 30.8 1.0 ND1 A:HIS-1 3.7 32.3 1.0 CB A:HIS136 3.7 27.0 1.0 CB A:HIS-1 3.7 28.4 1.0 O A:MET0 3.8 26.2 1.0 CA A:GLY138 3.8 19.7 1.0 N A:CYS137 3.8 24.0 1.0 C A:HIS-1 3.9 32.1 1.0 CG A:HIS136 4.0 32.0 1.0 CG A:HIS-1 4.1 28.8 1.0 CA A:HIS136 4.2 25.8 1.0 CA A:MET0 4.3 30.8 0.5 CA A:MET0 4.4 30.6 0.5 C A:CYS137 4.4 22.2 1.0 C A:MET0 4.4 28.8 1.0 C A:HIS136 4.4 24.4 1.0 C A:GLN140 4.5 30.9 1.0 O A:GLY138 4.5 23.2 1.0 C A:GLY138 4.5 20.9 1.0 CA A:CYS137 4.6 24.2 1.0 CB A:MET0 4.7 32.4 0.5 CE1 A:HIS136 4.7 35.2 1.0 CE1 A:HIS-1 4.8 34.3 1.0 CB A:MET0 4.8 32.5 0.5 N A:HIS-1 4.9 31.4 1.0 O A:GLY-2 4.9 24.0 1.0 CB A:CYS137 4.9 27.2 1.0

Zinc binding site 4 out of 4 in the Crystal Structure of the Effector ECP11-1 From Fulvia Fulva


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Crystal Structure of the Effector ECP11-1 From Fulvia Fulva within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn209 b:21.9 occ:0.80 OXT A:GLN140 1.6 24.9 1.0 ND1 A:HIS136 2.0 33.9 1.0 ND1 A:HIS-1 2.2 32.3 1.0 ZN A:ZN208 2.3 25.5 0.5 C A:GLN140 2.7 30.9 1.0 CE1 A:HIS136 3.0 35.2 1.0 O A:GLN140 3.0 29.4 1.0 CG A:HIS136 3.1 32.0 1.0 CE1 A:HIS-1 3.1 34.3 1.0 CG A:HIS-1 3.1 28.8 1.0 CB A:HIS136 3.4 27.0 1.0 CB A:HIS-1 3.4 28.4 1.0 CA A:HIS-1 4.1 30.8 1.0 NE2 A:HIS136 4.1 31.1 1.0 CA A:GLN140 4.2 30.6 1.0 CD2 A:HIS136 4.2 34.3 1.0 NE2 A:HIS-1 4.3 29.2 1.0 CD2 A:HIS-1 4.3 31.3 1.0 N A:GLN140 4.6 28.8 1.0 CA A:HIS136 4.6 25.8 1.0 O A:GLY138 4.6 23.2 1.0 N A:GLY138 4.7 20.3 1.0 CA A:GLY138 4.8 19.7 1.0 C A:GLY138 4.9 20.9 1.0 N A:MET0 4.9 27.9 1.0 CD2 A:LEU1 4.9 35.8 1.0 C A:HIS-1 4.9 32.1 1.0 CB A:GLN140 5.0 35.8 1.0

N.Lazar, C.Mesarich, Y.Petit-Houdenot, N.Talbi, I.Li De La Sierra-Gallay, E.Zelie, K.Blondeau, J.Gracy, B.Ollivier, A.Van De Wouw, M.H.Balesdent, A.Idnurm, H.Van Tilbeurgh, I.Fudal. AVRLM3, AVRLM4-7 and AVRLM5-9, Three Avirulence Effectors of Leptosphaeria Maculans Displaying Suppressive Interactions, Share A Structural Fold and Are Part of A Large Effector Family To Be Published.