Zinc in PDB 6yyz: Trna-Guanine Transglycosylase (Tgt) Labeled with 5-Fluorotryptophan in Co-Crystallized Complex with 6-Amino-2-(Methylamino)-4-(2-((2R,3R,4R, 5R)-3,4,5-Trimethoxytetrahydrofuran-2-Yl)Ethyl)-1,7-Dihydro-8H- Imidazo[4,5-G]Quinazolin-8-One

Enzymatic activity of Trna-Guanine Transglycosylase (Tgt) Labeled with 5-Fluorotryptophan in Co-Crystallized Complex with 6-Amino-2-(Methylamino)-4-(2-((2R,3R,4R, 5R)-3,4,5-Trimethoxytetrahydrofuran-2-Yl)Ethyl)-1,7-Dihydro-8H- Imidazo[4,5-G]Quinazolin-8-One

All present enzymatic activity of Trna-Guanine Transglycosylase (Tgt) Labeled with 5-Fluorotryptophan in Co-Crystallized Complex with 6-Amino-2-(Methylamino)-4-(2-((2R,3R,4R, 5R)-3,4,5-Trimethoxytetrahydrofuran-2-Yl)Ethyl)-1,7-Dihydro-8H- Imidazo[4,5-G]Quinazolin-8-One:
2.4.2.29;

Protein crystallography data

The structure of Trna-Guanine Transglycosylase (Tgt) Labeled with 5-Fluorotryptophan in Co-Crystallized Complex with 6-Amino-2-(Methylamino)-4-(2-((2R,3R,4R, 5R)-3,4,5-Trimethoxytetrahydrofuran-2-Yl)Ethyl)-1,7-Dihydro-8H- Imidazo[4,5-G]Quinazolin-8-One, PDB code: 6yyz was solved by A.Nguyen, A.Heine, G.Klebe, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 37.12 / 1.21
Space group C 1 2 1
Cell size a, b, c (Å), α, β, γ (°) 83.723, 65.036, 71.568, 90.00, 93.71, 90.00
R / Rfree (%) 13.4 / 15.9

Other elements in 6yyz:

The structure of Trna-Guanine Transglycosylase (Tgt) Labeled with 5-Fluorotryptophan in Co-Crystallized Complex with 6-Amino-2-(Methylamino)-4-(2-((2R,3R,4R, 5R)-3,4,5-Trimethoxytetrahydrofuran-2-Yl)Ethyl)-1,7-Dihydro-8H- Imidazo[4,5-G]Quinazolin-8-One also contains other interesting chemical elements:

Fluorine (F) 4 atoms

Zinc Binding Sites:

The binding sites of Zinc atom in the Trna-Guanine Transglycosylase (Tgt) Labeled with 5-Fluorotryptophan in Co-Crystallized Complex with 6-Amino-2-(Methylamino)-4-(2-((2R,3R,4R, 5R)-3,4,5-Trimethoxytetrahydrofuran-2-Yl)Ethyl)-1,7-Dihydro-8H- Imidazo[4,5-G]Quinazolin-8-One (pdb code 6yyz). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total only one binding site of Zinc was determined in the Trna-Guanine Transglycosylase (Tgt) Labeled with 5-Fluorotryptophan in Co-Crystallized Complex with 6-Amino-2-(Methylamino)-4-(2-((2R,3R,4R, 5R)-3,4,5-Trimethoxytetrahydrofuran-2-Yl)Ethyl)-1,7-Dihydro-8H- Imidazo[4,5-G]Quinazolin-8-One, PDB code: 6yyz:

Zinc binding site 1 out of 1 in 6yyz

Go back to Zinc Binding Sites List in 6yyz
Zinc binding site 1 out of 1 in the Trna-Guanine Transglycosylase (Tgt) Labeled with 5-Fluorotryptophan in Co-Crystallized Complex with 6-Amino-2-(Methylamino)-4-(2-((2R,3R,4R, 5R)-3,4,5-Trimethoxytetrahydrofuran-2-Yl)Ethyl)-1,7-Dihydro-8H- Imidazo[4,5-G]Quinazolin-8-One


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Trna-Guanine Transglycosylase (Tgt) Labeled with 5-Fluorotryptophan in Co-Crystallized Complex with 6-Amino-2-(Methylamino)-4-(2-((2R,3R,4R, 5R)-3,4,5-Trimethoxytetrahydrofuran-2-Yl)Ethyl)-1,7-Dihydro-8H- Imidazo[4,5-G]Quinazolin-8-One within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn401

b:21.8
occ:1.00
 ND1 A:HIS349 2.1 19.4 1.0
SG A:CYS323 2.3 21.6 1.0
SG A:CYS318 2.3 24.4 1.0
SG A:CYS320 2.3 25.2 1.0
CE1 A:HIS349 2.9 19.9 1.0
HE1 A:HIS349 3.0 23.9 1.0
HB2 A:CYS323 3.0 28.3 1.0
HB3 A:CYS318 3.0 30.1 1.0
HB3 A:CYS320 3.2 32.8 1.0
CB A:CYS318 3.2 25.1 1.0
CB A:CYS323 3.3 23.6 1.0
CG A:HIS349 3.3 19.9 1.0
H A:CYS323 3.3 28.9 1.0
HB2 A:HIS349 3.4 25.9 1.0
CB A:CYS320 3.4 27.3 1.0
H A:CYS320 3.4 36.2 1.0
HA A:HIS349 3.6 27.0 1.0
HB2 A:CYS318 3.6 30.1 1.0
CB A:HIS349 3.7 21.6 1.0
HB A:VAL322 3.9 31.3 1.0
N A:CYS323 3.9 24.1 1.0
HB3 A:CYS323 4.0 28.3 1.0
N A:CYS320 4.1 30.2 1.0
NE2 A:HIS349 4.1 19.8 1.0
HB2 A:LEU314 4.1 21.9 1.0
HB2 A:CYS320 4.1 32.8 1.0
CA A:HIS349 4.1 22.5 1.0
CA A:CYS323 4.2 24.6 1.0
CA A:CYS320 4.2 28.7 1.0
CD2 A:HIS349 4.3 19.7 1.0
HE22 A:GLN356 4.5 36.2 1.0
HB3 A:ASP315 4.5 27.5 1.0
H A:ASP315 4.5 24.1 1.0
HD12 A:LEU314 4.5 23.8 1.0
HB3 A:ALA352 4.5 32.5 1.0
O A:HIS349 4.6 22.6 1.0
CA A:CYS318 4.6 26.9 1.0
HA A:CYS323 4.6 29.5 1.0
HB3 A:HIS349 4.6 25.9 1.0
C A:CYS320 4.7 29.4 1.0
O A:CYS320 4.7 29.6 1.0
C A:CYS318 4.7 28.6 1.0
C A:HIS349 4.8 22.7 1.0
H A:VAL322 4.8 33.1 1.0
CB A:VAL322 4.9 26.1 1.0
HE2 A:HIS349 4.9 23.8 1.0
O A:CYS318 4.9 28.1 1.0
C A:VAL322 5.0 24.9 1.0

Reference:

A.Nguyen, A.Heine, G.Klebe. Co-Crystallization, 19F uc(Nmr) and Nanoesi-Ms Reveal Dimer Disturbing Inhibitors and Conformational Changes at Dimer Contacts To Be Published.
Page generated: Wed Dec 16 13:19:19 2020

Last articles

Zn in 8WB0
Zn in 8WAX
Zn in 8WAU
Zn in 8WAZ
Zn in 8WAY
Zn in 8WAV
Zn in 8WAW
Zn in 8WAT
Zn in 8W7M
Zn in 8WD3
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy