Zinc in PDB 6wib: Next Generation Monomeric IGG4 Fc

The structure of Next Generation Monomeric IGG4 Fc, PDB code: 6wib was solved by V.Y.Oganesyan, L.Shan, W.Dall'acqua, N.Van Dyk, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	35.92 / 2.55
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	101.306, 101.731, 69.044, 90, 90, 90
R / Rfree (%)	21.7 / 26.6

The binding sites of Zinc atom in the Next Generation Monomeric IGG4 Fc (pdb code 6wib). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 3 binding sites of Zinc where determined in the Next Generation Monomeric IGG4 Fc, PDB code: 6wib:
Jump to Zinc binding site number: 1; 2; 3;

Zinc binding site 1 out of 3 in the Next Generation Monomeric IGG4 Fc


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Next Generation Monomeric IGG4 Fc within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn501 b:43.2 occ:0.50 O A:HOH604 1.8 53.9 1.0 NE2 A:HIS285 2.6 46.4 1.0 CE1 A:HIS285 3.2 42.9 1.0 O A:HOH614 3.3 45.1 1.0 CD2 A:HIS285 3.8 43.7 1.0 ND1 A:HIS285 4.4 42.4 1.0 OE1 A:GLU283 4.6 43.8 1.0 CG A:HIS285 4.7 44.7 1.0 CD A:GLU283 4.9 43.0 1.0

Zinc binding site 2 out of 3 in the Next Generation Monomeric IGG4 Fc


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Next Generation Monomeric IGG4 Fc within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn502 b:28.5 occ:1.00 NE2 A:HIS435 1.9 26.4 1.0 NE2 A:HIS310 2.0 26.6 1.0 CD2 A:HIS435 2.9 24.8 1.0 CE1 A:HIS310 2.9 28.4 1.0 CE1 A:HIS435 3.0 24.1 1.0 CD2 A:HIS310 3.0 27.7 1.0 CD1 A:LEU314 3.9 27.3 1.0 ND1 A:HIS435 4.0 26.3 1.0 CG A:HIS435 4.0 26.4 1.0 ND1 A:HIS310 4.1 27.0 1.0 CG A:HIS310 4.1 27.6 1.0

Zinc binding site 3 out of 3 in the Next Generation Monomeric IGG4 Fc


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Next Generation Monomeric IGG4 Fc within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn503 b:81.4 occ:1.00 OE2 A:GLU345 2.4 47.5 1.0 CD A:GLU345 3.2 40.7 1.0 OE1 A:GLU345 3.4 38.1 1.0 O A:ALA431 4.4 32.0 1.0 CG A:GLU345 4.5 40.1 1.0 CB A:GLU345 4.8 37.2 1.0

L.Shan, N.Van Dyk, N.Haskins, K.M.Cook, K.L.Rosenthal, R.Mazor, S.Dragulin-Otto, Y.Jiang, H.Wu, W.F.Dall'acqua, M.J.Borrok, M.M.Damschroder, V.Oganesyan. In Vivo Pharmacokinetic Enhancement of Monomeric Fc and Monovalent Bispecific Designs Through Structural Guidance Commun Biol V. 4 2021.
ISSN: ESSN 2399-3642
DOI: 10.1038/S42003-021-02565-5