Zinc in PDB 6t5k: Ecv-1 From Echinicola Vietnamensis. Environmental Metallo-Beta- Lactamases Exhibit High Enzymatic Activity Under Zinc Deprivation

The structure of Ecv-1 From Echinicola Vietnamensis. Environmental Metallo-Beta- Lactamases Exhibit High Enzymatic Activity Under Zinc Deprivation, PDB code: 6t5k was solved by C.Frohlich, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	25.00 / 1.33
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	51.926, 65.676, 128.501, 90.00, 90.00, 90.00
R / Rfree (%)	14.7 / 18.4

The binding sites of Zinc atom in the Ecv-1 From Echinicola Vietnamensis. Environmental Metallo-Beta- Lactamases Exhibit High Enzymatic Activity Under Zinc Deprivation (pdb code 6t5k). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Ecv-1 From Echinicola Vietnamensis. Environmental Metallo-Beta- Lactamases Exhibit High Enzymatic Activity Under Zinc Deprivation, PDB code: 6t5k:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Ecv-1 From Echinicola Vietnamensis. Environmental Metallo-Beta- Lactamases Exhibit High Enzymatic Activity Under Zinc Deprivation


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Ecv-1 From Echinicola Vietnamensis. Environmental Metallo-Beta- Lactamases Exhibit High Enzymatic Activity Under Zinc Deprivation within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn1206 b:18.4 occ:0.97 O C:HOH1336 1.9 25.9 1.0 NE2 C:HIS196 2.0 15.9 1.0 ND1 C:HIS118 2.0 16.3 1.0 NE2 C:HIS116 2.1 15.7 1.0 HB2 C:HIS118 2.9 21.8 1.0 CD2 C:HIS196 3.0 16.2 1.0 CE1 C:HIS116 3.0 16.1 1.0 CE1 C:HIS118 3.0 17.8 1.0 CE1 C:HIS196 3.0 17.2 1.0 CG C:HIS118 3.0 17.5 1.0 CD2 C:HIS116 3.1 15.7 1.0 HD2 C:HIS196 3.1 19.4 1.0 O C:HOH1460 3.1 35.2 1.0 HE1 C:HIS116 3.1 19.3 1.0 HE1 C:HIS118 3.2 21.4 1.0 HE1 C:HIS196 3.2 20.6 1.0 HD2 C:HIS116 3.3 18.9 1.0 CB C:HIS118 3.4 18.1 1.0 ZN C:ZN1207 3.5 20.8 0.5 HB3 C:HIS118 3.6 21.8 1.0 HB2 C:CYS221 3.7 24.7 1.0 OD1 C:ASP120 3.8 25.3 1.0 HG23 C:THR197 4.1 19.3 1.0 O C:HOH1438 4.1 34.0 1.0 ND1 C:HIS116 4.1 17.9 1.0 NE2 C:HIS118 4.1 18.8 1.0 ND1 C:HIS196 4.1 16.4 1.0 CG C:HIS196 4.1 15.4 1.0 SG C:CYS221 4.1 23.4 1.0 CD2 C:HIS118 4.2 18.2 1.0 CG C:HIS116 4.2 16.8 1.0 HB3 C:CYS221 4.2 24.7 1.0 CB C:CYS221 4.2 20.5 1.0 HG21 C:THR197 4.3 19.3 1.0 OD2 C:ASP120 4.5 24.6 1.0 HD22 C:ASN233 4.5 38.3 1.0 CG C:ASP120 4.6 24.6 1.0 H C:HIS118 4.6 21.2 1.0 CG2 C:THR197 4.6 16.1 1.0 CA C:HIS118 4.8 18.0 1.0 HD1 C:HIS116 4.8 21.5 1.0 HE2 C:HIS118 4.9 22.6 1.0 HD1 C:HIS196 4.9 19.7 1.0 HG22 C:THR197 5.0 19.3 1.0

Zinc binding site 2 out of 2 in the Ecv-1 From Echinicola Vietnamensis. Environmental Metallo-Beta- Lactamases Exhibit High Enzymatic Activity Under Zinc Deprivation


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Ecv-1 From Echinicola Vietnamensis. Environmental Metallo-Beta- Lactamases Exhibit High Enzymatic Activity Under Zinc Deprivation within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn1207 b:20.8 occ:0.54 SG C:CYS221 2.1 23.4 1.0 O C:HOH1336 2.1 25.9 1.0 NE2 C:HIS263 2.2 26.5 1.0 OD2 C:ASP120 2.3 24.6 1.0 O C:HOH1438 2.3 34.0 1.0 HB3 C:CYS221 3.1 24.7 1.0 CE1 C:HIS263 3.1 27.8 1.0 CD2 C:HIS263 3.2 26.4 1.0 CB C:CYS221 3.2 20.5 1.0 HE1 C:HIS263 3.3 33.4 1.0 CG C:ASP120 3.3 24.6 1.0 HD2 C:HIS263 3.4 31.6 1.0 ZN C:ZN1206 3.5 18.4 1.0 HB2 C:CYS221 3.6 24.7 1.0 OD1 C:ASP120 3.6 25.3 1.0 HE1 C:HIS116 3.7 19.3 1.0 O C:HOH1460 3.9 35.2 1.0 O C:HOH1425 4.0 23.4 1.0 NE2 C:HIS196 4.0 15.9 1.0 HE1 C:HIS196 4.2 20.6 1.0 ND1 C:HIS263 4.3 26.9 1.0 CE1 C:HIS196 4.3 17.2 1.0 CG C:HIS263 4.3 24.4 1.0 CE1 C:HIS116 4.3 16.1 1.0 HA C:CYS221 4.4 21.0 1.0 NE2 C:HIS116 4.4 15.7 1.0 CA C:CYS221 4.5 17.5 1.0 O C:HOH1479 4.5 36.4 1.0 HA3 C:GLY262 4.6 21.2 1.0 CB C:ASP120 4.7 23.4 1.0 HB2 C:ASP120 4.7 28.1 1.0 O C:HOH1307 4.8 30.5 1.0 CD2 C:HIS196 4.9 16.2 1.0 O C:HOH1345 4.9 27.4 1.0 H C:CYS221 4.9 21.1 1.0

C.Frohlich, V.Sorum, S.Huber, O.Samuelsen, F.Berglund, E.Kristiansson, S.Kotsakis, M.P.Nachiket, J.D.G.Larsson, H.K.S.Leiros. Environmental Metallo-Beta-Lactamases Exhibit High Enzymatic Activity Under Zinc Deprivation To Be Published.