Zinc in PDB 6oul: Cryo-Em Structure of Escherichia Coli Rnap Polymerase Bound to RPSTP2 Promoter Dna

All present enzymatic activity of Cryo-Em Structure of Escherichia Coli Rnap Polymerase Bound to RPSTP2 Promoter Dna:
2.7.7.6;

The structure of Cryo-Em Structure of Escherichia Coli Rnap Polymerase Bound to RPSTP2 Promoter Dna also contains other interesting chemical elements:

Magnesium

(Mg)

1 atom

The binding sites of Zinc atom in the Cryo-Em Structure of Escherichia Coli Rnap Polymerase Bound to RPSTP2 Promoter Dna (pdb code 6oul). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Cryo-Em Structure of Escherichia Coli Rnap Polymerase Bound to RPSTP2 Promoter Dna, PDB code: 6oul:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Cryo-Em Structure of Escherichia Coli Rnap Polymerase Bound to RPSTP2 Promoter Dna


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Cryo-Em Structure of Escherichia Coli Rnap Polymerase Bound to RPSTP2 Promoter Dna within 5.0Å range: probe atom residue distance (Å) B Occ J:Zn1502 b:82.3 occ:1.00 SG J:CYS70 2.3 26.3 1.0 SG J:CYS85 2.3 24.8 1.0 SG J:CYS72 2.4 30.7 1.0 SG J:CYS88 2.4 29.6 1.0 CB J:CYS85 2.8 24.8 1.0 N J:CYS88 3.3 29.6 1.0 CB J:CYS72 3.4 30.7 1.0 CB J:CYS88 3.7 29.6 1.0 N J:CYS72 3.8 30.7 1.0 CA J:CYS88 3.9 29.6 1.0 CB J:CYS70 3.9 26.3 1.0 N J:GLY89 4.1 21.8 1.0 CB J:LYS87 4.2 33.9 1.0 CA J:CYS72 4.2 30.7 1.0 C J:CYS88 4.2 29.6 1.0 CA J:CYS85 4.3 24.8 1.0 N J:LYS87 4.3 33.9 1.0 C J:LYS87 4.3 33.9 1.0 CA J:LYS87 4.5 33.9 1.0 N J:LEU71 4.5 22.6 1.0 C J:CYS85 4.8 24.8 1.0 N J:VAL90 4.8 18.7 1.0 N J:GLU86 4.9 31.9 1.0 CB J:LYS74 4.9 35.6 1.0 C J:CYS72 4.9 30.7 1.0 C J:LEU71 5.0 22.6 1.0

Zinc binding site 2 out of 2 in the Cryo-Em Structure of Escherichia Coli Rnap Polymerase Bound to RPSTP2 Promoter Dna


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Cryo-Em Structure of Escherichia Coli Rnap Polymerase Bound to RPSTP2 Promoter Dna within 5.0Å range: probe atom residue distance (Å) B Occ J:Zn1503 b:71.7 occ:1.00 SG J:CYS888 2.3 24.4 1.0 SG J:CYS895 2.3 18.0 1.0 SG J:CYS898 2.3 19.7 1.0 SG J:CYS814 2.4 26.7 1.0 CB J:CYS898 2.5 19.7 1.0 CB J:CYS888 3.4 24.4 1.0 CB J:CYS895 3.7 18.0 1.0 CB J:CYS814 3.8 26.7 1.0 CA J:CYS888 3.8 24.4 1.0 CA J:CYS898 3.8 19.7 1.0 N J:CYS895 4.0 18.0 1.0 N J:CYS898 4.1 19.7 1.0 N J:ASP889 4.2 30.2 1.0 OG1 J:THR890 4.3 24.7 1.0 NH2 J:ARG883 4.3 21.9 1.0 CA J:CYS895 4.3 18.0 1.0 C J:CYS888 4.5 24.4 1.0 N J:CYS814 4.6 26.7 1.0 O J:CYS895 4.6 18.0 1.0 N J:THR890 4.8 24.7 1.0 CA J:CYS814 4.8 26.7 1.0 C J:CYS898 4.8 19.7 1.0 C J:CYS895 4.8 18.0 1.0 OG1 J:THR816 4.9 36.0 1.0

J.Chen, S.Gopalkrishnan, C.Chiu, A.Y.Chen, E.A.Campbell, R.L.Gourse, W.Ross, S.A.Darst. E. Colitrar Allosterically Regulates Transcription Initiation By Altering Rna Polymerase Conformation. Elife V. 8 2019.
ISSN: ESSN 2050-084X
PubMed: 31841111
DOI: 10.7554/ELIFE.49375