Zinc in PDB 6nwv: Insulin Lispro Analog

The structure of Insulin Lispro Analog, PDB code: 6nwv was solved by A.Cruz-Rangel, A.Romero, J.P.Reyes-Grajeda, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	42.96 / 1.60
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	46.161, 61.789, 58.618, 90.00, 111.46, 90.00
R / Rfree (%)	20.8 / 25.7

The structure of Insulin Lispro Analog also contains other interesting chemical elements:

Chlorine

(Cl)

2 atoms

The binding sites of Zinc atom in the Insulin Lispro Analog (pdb code 6nwv). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Insulin Lispro Analog, PDB code: 6nwv:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Insulin Lispro Analog


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Insulin Lispro Analog within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn101 b:21.7 occ:1.00 NE2 L:HIS10 1.9 20.9 1.0 NE2 B:HIS10 1.9 19.5 1.0 NE2 D:HIS10 2.1 23.1 1.0 CL L:CL101 2.4 43.6 1.0 CE1 L:HIS10 2.7 24.4 1.0 CE1 B:HIS10 2.8 25.0 1.0 CE1 D:HIS10 2.9 22.0 1.0 CD2 B:HIS10 3.1 20.8 1.0 CD2 L:HIS10 3.1 22.4 1.0 CD2 D:HIS10 3.2 21.8 1.0 ND1 L:HIS10 3.9 25.4 1.0 ND1 B:HIS10 3.9 22.8 1.0 ND1 D:HIS10 4.0 22.4 1.0 CG L:HIS10 4.1 21.7 1.0 CG B:HIS10 4.1 22.1 1.0 CG D:HIS10 4.2 22.7 1.0 O B:LEU6 4.7 24.6 1.0 O B:HOH204 4.8 34.3 1.0 O L:HOH201 4.9 31.7 1.0 O L:LEU6 4.9 24.4 1.0 CB L:LEU6 4.9 22.0 1.0 CB B:LEU6 5.0 26.5 1.0 CB D:LEU6 5.0 23.3 1.0

Zinc binding site 2 out of 2 in the Insulin Lispro Analog


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Insulin Lispro Analog within 5.0Å range: probe atom residue distance (Å) B Occ F:Zn101 b:21.9 occ:1.00 NE2 H:HIS10 1.9 22.5 1.0 NE2 J:HIS10 2.0 22.9 1.0 NE2 F:HIS10 2.2 21.8 1.0 CL J:CL101 2.5 46.7 1.0 CE1 H:HIS10 2.8 26.8 1.0 CE1 F:HIS10 2.8 23.9 1.0 CE1 J:HIS10 2.9 22.8 1.0 CD2 H:HIS10 3.0 20.8 1.0 CD2 J:HIS10 3.1 22.1 1.0 CD2 F:HIS10 3.3 22.3 1.0 ND1 H:HIS10 3.9 23.6 1.0 ND1 F:HIS10 4.0 24.0 1.0 ND1 J:HIS10 4.0 23.1 1.0 CG H:HIS10 4.1 24.8 1.0 CG J:HIS10 4.2 23.3 1.0 CG F:HIS10 4.2 21.3 1.0 O H:HOH102 4.9 39.9 1.0 CB H:LEU6 4.9 21.5 1.0 O J:LEU6 4.9 25.6 1.0 O F:LEU6 4.9 22.7 1.0 OG J:SER9 4.9 36.0 0.5 O J:HOH201 5.0 30.1 1.0 O H:LEU6 5.0 21.5 1.0 CB J:LEU6 5.0 27.0 1.0

A.Cruz-Rangel, A.Romero, J.P.Reyes-Grajeda. Crystal Structure of Lispro Hexameric Insulin Analog To Be Published.