Zinc in PDB 6mus: Cryo-Em Structure of Larger Csm-Crrna-Target Rna Ternary Complex in Type III-A Crispr-Cas System

The binding sites of Zinc atom in the Cryo-Em Structure of Larger Csm-Crrna-Target Rna Ternary Complex in Type III-A Crispr-Cas System (pdb code 6mus). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 4 binding sites of Zinc where determined in the Cryo-Em Structure of Larger Csm-Crrna-Target Rna Ternary Complex in Type III-A Crispr-Cas System, PDB code: 6mus:
Jump to Zinc binding site number: 1; 2; 3; 4;

Zinc binding site 1 out of 4 in the Cryo-Em Structure of Larger Csm-Crrna-Target Rna Ternary Complex in Type III-A Crispr-Cas System


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Cryo-Em Structure of Larger Csm-Crrna-Target Rna Ternary Complex in Type III-A Crispr-Cas System within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn801 b:41.1 occ:1.00 SG A:CYS389 2.4 21.6 1.0 SG A:CYS416 2.4 33.9 1.0 SG A:CYS413 2.5 33.9 1.0 SG A:CYS392 2.5 20.7 1.0 CB A:CYS413 2.5 33.9 1.0 N A:CYS413 3.3 33.9 1.0 CB A:CYS389 3.3 21.6 1.0 CA A:CYS413 3.5 33.9 1.0 CB A:CYS416 3.7 33.9 1.0 CB A:CYS392 3.9 20.7 1.0 N A:CYS392 4.2 20.7 1.0 C A:CYS413 4.5 33.9 1.0 C A:VAL412 4.6 37.6 1.0 CA A:CYS392 4.6 20.7 1.0 N A:CYS416 4.6 33.9 1.0 CA A:CYS416 4.7 33.9 1.0 CB A:VAL391 4.7 15.7 1.0 CG2 A:THR415 4.8 27.9 1.0 O A:CYS413 4.8 33.9 1.0 CA A:CYS389 4.8 21.6 1.0 CB A:VAL412 4.9 37.6 1.0 CA A:VAL412 5.0 37.6 1.0

Zinc binding site 2 out of 4 in the Cryo-Em Structure of Larger Csm-Crrna-Target Rna Ternary Complex in Type III-A Crispr-Cas System


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Cryo-Em Structure of Larger Csm-Crrna-Target Rna Ternary Complex in Type III-A Crispr-Cas System within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn301 b:34.0 occ:1.00 CE1 C:HIS111 2.3 14.2 1.0 SG C:CYS125 2.4 15.5 1.0 SG C:CYS122 2.4 16.9 1.0 SG C:CYS113 2.5 13.8 1.0 CB C:CYS125 2.9 15.5 1.0 CB C:CYS122 2.9 16.9 1.0 ND1 C:HIS111 3.3 14.2 1.0 NE2 C:HIS111 3.3 14.2 1.0 N C:CYS125 3.4 15.5 1.0 CA C:CYS125 3.7 15.5 1.0 CB C:CYS113 3.8 13.8 1.0 CA C:CYS122 4.2 16.9 1.0 N C:CYS113 4.3 13.8 1.0 O C:CYS122 4.4 16.9 1.0 C C:CYS122 4.4 16.9 1.0 CG C:HIS111 4.5 14.2 1.0 CD2 C:HIS111 4.5 14.2 1.0 CA C:CYS113 4.5 13.8 1.0 C C:VAL124 4.5 19.2 1.0 CB C:VAL124 4.6 19.2 1.0 N C:VAL124 4.7 19.2 1.0 CA C:VAL124 4.8 19.2 1.0 C C:CYS125 4.9 15.5 1.0

Zinc binding site 3 out of 4 in the Cryo-Em Structure of Larger Csm-Crrna-Target Rna Ternary Complex in Type III-A Crispr-Cas System


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Cryo-Em Structure of Larger Csm-Crrna-Target Rna Ternary Complex in Type III-A Crispr-Cas System within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn301 b:49.5 occ:1.00 NE2 D:HIS111 2.1 15.4 1.0 SG D:CYS113 2.4 13.9 1.0 SG D:CYS122 2.5 12.3 1.0 SG D:CYS125 2.6 13.3 1.0 CE1 D:HIS111 2.7 15.4 1.0 CB D:CYS125 3.2 13.3 1.0 CD2 D:HIS111 3.2 15.4 1.0 CB D:CYS122 3.2 12.3 1.0 CB D:CYS113 3.5 13.9 1.0 N D:CYS125 3.8 13.3 1.0 N D:CYS113 3.8 13.9 1.0 ND1 D:HIS111 3.9 15.4 1.0 CA D:CYS125 4.1 13.3 1.0 CA D:CYS113 4.2 13.9 1.0 CG D:HIS111 4.2 15.4 1.0 CB D:VAL124 4.5 15.6 1.0 CA D:CYS122 4.7 12.3 1.0 C D:VAL112 4.7 16.4 1.0 C D:VAL124 4.8 15.6 1.0 OH D:TYR84 4.9 22.1 1.0 N D:VAL124 5.0 15.6 1.0

Zinc binding site 4 out of 4 in the Cryo-Em Structure of Larger Csm-Crrna-Target Rna Ternary Complex in Type III-A Crispr-Cas System


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Cryo-Em Structure of Larger Csm-Crrna-Target Rna Ternary Complex in Type III-A Crispr-Cas System within 5.0Å range: probe atom residue distance (Å) B Occ K:Zn301 b:54.7 occ:1.00 NE2 K:HIS111 2.2 38.7 1.0 SG K:CYS122 2.4 33.4 1.0 SG K:CYS113 2.5 36.3 1.0 SG K:CYS125 2.5 27.9 1.0 CD2 K:HIS111 2.7 38.7 1.0 CE1 K:HIS111 3.2 38.7 1.0 CB K:CYS125 3.4 27.9 1.0 CB K:CYS113 3.5 36.3 1.0 CB K:CYS122 3.5 33.4 1.0 N K:CYS113 3.6 36.3 1.0 CG K:HIS111 3.8 38.7 1.0 ND1 K:HIS111 4.0 38.7 1.0 N K:CYS125 4.0 27.9 1.0 CA K:CYS113 4.1 36.3 1.0 CA K:CYS125 4.3 27.9 1.0 C K:VAL112 4.4 36.1 1.0 O K:HIS111 4.5 38.7 1.0 CB K:VAL124 4.5 26.8 1.0 CA K:VAL112 4.7 36.1 1.0 C K:HIS111 4.8 38.7 1.0 N K:VAL112 4.9 36.1 1.0 CA K:CYS122 4.9 33.4 1.0

N.Jia, C.Y.Mo, C.Wang, E.T.Eng, L.A.Marraffini, D.J.Patel. Type III-A Crispr-Cas Csm Complexes: Assembly, Periodic Rna Cleavage, Dnase Activity Regulation, and Autoimmunity. Mol. Cell V. 73 264 2019.
ISSN: ISSN 1097-4164
PubMed: 30503773
DOI: 10.1016/J.MOLCEL.2018.11.007