Zinc in PDB 6mf0: Crystal Structure Determination of Human/Porcine Chimera Coagulation Factor VIII

The structure of Crystal Structure Determination of Human/Porcine Chimera Coagulation Factor VIII, PDB code: 6mf0 was solved by I.W.Smith, P.C.Spiegel, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	49.03 / 3.20
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	72.005, 135.863, 196.107, 90.00, 90.15, 90.00
R / Rfree (%)	20.6 / 28.7

The structure of Crystal Structure Determination of Human/Porcine Chimera Coagulation Factor VIII also contains other interesting chemical elements:

Copper	(Cu)	2 atoms
Calcium	(Ca)	2 atoms

The binding sites of Zinc atom in the Crystal Structure Determination of Human/Porcine Chimera Coagulation Factor VIII (pdb code 6mf0). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure Determination of Human/Porcine Chimera Coagulation Factor VIII, PDB code: 6mf0:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure Determination of Human/Porcine Chimera Coagulation Factor VIII


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure Determination of Human/Porcine Chimera Coagulation Factor VIII within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn2415 b:46.1 occ:1.00 ND1 A:HIS268 2.0 52.0 1.0 ND1 A:HIS318 2.1 61.4 1.0 SG A:CYS311 2.3 38.7 1.0 CE1 A:HIS268 2.7 51.2 1.0 CE1 A:HIS318 3.0 59.1 1.0 CG A:HIS318 3.0 64.8 1.0 CG A:HIS268 3.2 48.0 1.0 CB A:CYS311 3.3 47.1 1.0 CB A:HIS318 3.4 75.3 1.0 CG A:MET321 3.7 50.8 1.0 CB A:HIS268 3.8 38.8 1.0 NE2 A:HIS268 3.9 48.6 1.0 CA A:HIS318 4.0 82.3 1.0 NE2 A:HIS318 4.1 68.5 1.0 O A:VAL267 4.1 48.7 1.0 CA A:HIS268 4.1 41.9 1.0 CD2 A:HIS318 4.1 67.9 1.0 CD2 A:HIS268 4.2 50.2 1.0 CE A:MET321 4.2 45.9 1.0 CG1 A:ILE313 4.4 53.4 1.0 CB A:MET321 4.6 48.2 1.0 SD A:MET321 4.6 54.2 1.0 C A:HIS318 4.7 83.3 1.0 CA A:CYS311 4.8 46.1 1.0 C A:VAL267 4.9 45.9 1.0 CB A:ILE313 4.9 59.0 1.0 CG A:HIS233 4.9 47.3 1.0 N A:HIS268 5.0 47.2 1.0 CD2 A:HIS233 5.0 60.5 1.0

Zinc binding site 2 out of 2 in the Crystal Structure Determination of Human/Porcine Chimera Coagulation Factor VIII


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure Determination of Human/Porcine Chimera Coagulation Factor VIII within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn2420 b:47.1 occ:1.00 ND1 B:HIS268 2.1 46.6 1.0 SG B:CYS311 2.4 45.5 1.0 CB B:HIS318 2.7 65.5 1.0 ND1 B:HIS318 2.8 77.8 1.0 O B:HIS318 2.9 71.9 1.0 CE1 B:HIS268 2.9 48.1 1.0 CG B:HIS318 3.1 61.2 1.0 CG B:HIS268 3.2 41.4 1.0 CA B:HIS318 3.2 74.3 1.0 C B:HIS318 3.5 75.3 1.0 CB B:CYS311 3.6 49.8 1.0 CB B:HIS268 3.7 40.3 1.0 ND1 B:HIS233 3.7 54.6 1.0 O B:VAL267 3.9 56.2 1.0 CE1 B:HIS233 4.0 58.1 1.0 SD B:MET321 4.0 53.4 1.0 NE2 B:HIS268 4.1 38.0 1.0 CE1 B:HIS318 4.1 88.8 1.0 CA B:HIS268 4.1 44.3 1.0 CD2 B:HIS268 4.2 37.8 1.0 CD2 B:HIS318 4.5 61.3 1.0 CG B:MET321 4.6 58.4 1.0 N B:HIS318 4.7 75.8 1.0 CB B:ILE313 4.7 56.5 1.0 C B:VAL267 4.8 51.4 1.0 N B:GLY319 4.8 79.1 1.0 N B:HIS268 4.9 50.3 1.0 NE2 B:HIS318 4.9 81.7 1.0 CG B:HIS233 5.0 58.2 1.0 CA B:CYS311 5.0 43.7 1.0

I.W.Smith, A.E.D'aquino, C.W.Coyle, A.Fedanov, E.T.Parker, G.Denning, H.T.Spencer, P.Lollar, C.B.Doering, P.C.Spiegel Jr.. The 3.2 Angstrom Structure of A Bioengineered Variant of Blood Coagulation Factor VIII Indicates Two Conformations of the C2 Domain. J.Thromb.Haemost. 2019.
ISSN: ESSN 1538-7836
PubMed: 31454152
DOI: 10.1111/JTH.14621