Zinc in PDB 6koq: Mycobacterium Tuberculosis Initial Transcription Complex Comprising Sigma H and 5'-Oh Rna of 10 Nt

All present enzymatic activity of Mycobacterium Tuberculosis Initial Transcription Complex Comprising Sigma H and 5'-Oh Rna of 10 Nt:
2.7.7.6;

The structure of Mycobacterium Tuberculosis Initial Transcription Complex Comprising Sigma H and 5'-Oh Rna of 10 Nt, PDB code: 6koq was solved by L.Li, Y.Zhang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	36.51 / 3.35
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	126.846, 161.097, 129.551, 90.00, 117.44, 90.00
R / Rfree (%)	22.1 / 26

The structure of Mycobacterium Tuberculosis Initial Transcription Complex Comprising Sigma H and 5'-Oh Rna of 10 Nt also contains other interesting chemical elements:

Magnesium

(Mg)

1 atom

The binding sites of Zinc atom in the Mycobacterium Tuberculosis Initial Transcription Complex Comprising Sigma H and 5'-Oh Rna of 10 Nt (pdb code 6koq). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Mycobacterium Tuberculosis Initial Transcription Complex Comprising Sigma H and 5'-Oh Rna of 10 Nt, PDB code: 6koq:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Mycobacterium Tuberculosis Initial Transcription Complex Comprising Sigma H and 5'-Oh Rna of 10 Nt


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Mycobacterium Tuberculosis Initial Transcription Complex Comprising Sigma H and 5'-Oh Rna of 10 Nt within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn2001 b:0.8 occ:1.00 SG D:CYS978 2.3 0.2 1.0 SG D:CYS891 2.3 0.5 1.0 SG D:CYS968 2.3 0.9 1.0 SG D:CYS975 2.4 95.0 1.0 CB D:CYS978 2.5 0.2 1.0 CB D:CYS891 2.8 0.0 1.0 CB D:CYS968 2.9 0.8 1.0 CA D:CYS968 3.1 0.9 1.0 N D:ALA969 3.7 0.8 1.0 CB D:CYS975 3.8 1.0 1.0 C D:CYS968 3.9 0.2 1.0 CA D:CYS978 4.0 0.7 1.0 CA D:CYS891 4.0 0.5 1.0 N D:CYS891 4.1 0.3 1.0 N D:CYS968 4.3 96.9 1.0 CG2 D:THR893 4.4 0.1 1.0 NH1 D:ARG963 4.4 0.4 1.0 N D:CYS975 4.4 98.9 1.0 N D:CYS978 4.4 0.2 1.0 CA D:CYS975 4.6 0.4 1.0 O D:SER964 4.8 95.5 1.0 O D:CYS975 4.8 0.2 1.0 C D:CYS978 4.9 0.3 1.0 N D:THR970 5.0 0.5 1.0 CA D:ALA969 5.0 0.7 1.0

Zinc binding site 2 out of 2 in the Mycobacterium Tuberculosis Initial Transcription Complex Comprising Sigma H and 5'-Oh Rna of 10 Nt


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Mycobacterium Tuberculosis Initial Transcription Complex Comprising Sigma H and 5'-Oh Rna of 10 Nt within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn2002 b:0.2 occ:1.00 SG D:CYS62 2.3 0.2 1.0 SG D:CYS78 2.3 0.6 1.0 SG D:CYS75 2.3 0.9 1.0 SG D:CYS60 2.4 0.2 1.0 CB D:CYS78 2.8 0.5 1.0 CB D:CYS60 3.0 0.9 1.0 CB D:CYS75 3.4 0.5 1.0 N D:CYS62 3.5 0.3 1.0 N D:CYS78 3.6 0.7 1.0 CB D:CYS62 3.7 0.9 1.0 CA D:CYS78 3.8 0.3 1.0 N D:TYR61 4.0 0.5 1.0 CA D:CYS62 4.1 0.3 1.0 N D:GLY63 4.2 0.1 1.0 CA D:CYS60 4.2 0.5 1.0 N D:LYS64 4.2 0.9 1.0 C D:CYS60 4.3 0.3 1.0 CB D:LYS64 4.4 0.6 1.0 C D:CYS62 4.5 0.8 1.0 C D:TYR61 4.5 0.3 1.0 CB D:ARG77 4.6 0.9 1.0 CD1 D:TYR65 4.7 0.0 1.0 C D:ARG77 4.7 0.7 1.0 CA D:TYR61 4.8 0.4 1.0 CA D:CYS75 4.8 0.1 1.0 C D:CYS78 4.9 0.3 1.0 CA D:LYS64 4.9 0.6 1.0

L.Li, V.Molodtsov, W.Lin, R.H.Ebright, Y.Zhang. Rna Extension Drives A Stepwise Displacement of An Initiation-Factor Structural Module in Initial Transcription Proc.Natl.Acad.Sci.Usa 2020.
ISSN: ESSN 1091-6490
DOI: 10.1073/PNAS.1920747117