Zinc in PDB 6hyg: Heteromeric Tandem IGG4/IGG1 Fc

The structure of Heteromeric Tandem IGG4/IGG1 Fc, PDB code: 6hyg was solved by J.B.Casaletto, M.L.Geddie, A.O.Abu-Yousif, K.Masson, A.Fulgham, A.Boudot, T.Maiwald, J.D.Kearns, N.Kohli, S.Su, M.Razlog, A.Raue, A.Kalra, M.Hakansson, D.T.Logan, M.Welin, S.Chattopadhyay, B.D.Harms, U.B.Nielsen, B.Schoeberl, A.A.Lugovskoy, G.Macbeath, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.28 / 2.31
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	136.119, 63.253, 77.551, 90.00, 90.00, 90.00
R / Rfree (%)	25.8 / 27.7

The binding sites of Zinc atom in the Heteromeric Tandem IGG4/IGG1 Fc (pdb code 6hyg). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Heteromeric Tandem IGG4/IGG1 Fc, PDB code: 6hyg:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Heteromeric Tandem IGG4/IGG1 Fc


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Heteromeric Tandem IGG4/IGG1 Fc within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn501 b:40.8 occ:1.00 NE2 A:HIS90 1.9 36.1 1.0 NE2 A:HIS215 2.1 46.7 1.0 CE1 A:HIS90 2.7 38.3 1.0 CE1 A:HIS215 3.1 41.6 1.0 CD2 A:HIS215 3.1 41.2 1.0 CD2 A:HIS90 3.1 40.1 1.0 ND1 A:HIS90 3.9 37.4 1.0 CD1 A:LEU94 4.0 43.4 1.0 CG A:HIS90 4.1 39.2 1.0 ND1 A:HIS215 4.1 42.4 1.0 CG A:HIS215 4.2 39.2 1.0

Zinc binding site 2 out of 2 in the Heteromeric Tandem IGG4/IGG1 Fc


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Heteromeric Tandem IGG4/IGG1 Fc within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn502 b:40.0 occ:1.00 NE2 A:HIS355 2.0 35.0 1.0 O A:HOH678 2.1 28.9 1.0 NE2 A:HIS480 2.1 43.0 1.0 CE1 A:HIS355 2.9 37.0 1.0 CD2 A:HIS355 3.1 38.5 1.0 CD2 A:HIS480 3.1 40.8 1.0 CE1 A:HIS480 3.1 40.6 1.0 CD1 A:LEU359 4.0 40.5 1.0 ND1 A:HIS355 4.1 36.3 1.0 CG A:HIS355 4.2 38.7 1.0 ND1 A:HIS480 4.3 40.6 1.0 CG A:HIS480 4.3 39.1 1.0

J.B.Casaletto, M.L.Geddie, A.O.Abu-Yousif, K.Masson, A.Fulgham, A.Boudot, T.Maiwald, J.D.Kearns, N.Kohli, S.Su, M.Razlog, A.Raue, A.Kalra, M.Hakansson, D.T.Logan, M.Welin, S.Chattopadhyay, B.D.Harms, U.B.Nielsen, B.Schoeberl, A.A.Lugovskoy, G.Macbeath. Mm-131, A Bispecific Anti-Met/Epcam Mab, Inhibits Hgf-Dependent and Hgf-Independent Met Signaling Through Concurrent Binding to Epcam. Proc.Natl.Acad.Sci.Usa V. 116 7533 2019.
ISSN: ESSN 1091-6490
PubMed: 30898885
DOI: 10.1073/PNAS.1819085116