Zinc in PDB 6hv8: Cryo-Em Structure of S. Cerevisiae Polymerase Epsilon Deltacat Mutant

All present enzymatic activity of Cryo-Em Structure of S. Cerevisiae Polymerase Epsilon Deltacat Mutant:
2.7.7.7;

The binding sites of Zinc atom in the Cryo-Em Structure of S. Cerevisiae Polymerase Epsilon Deltacat Mutant (pdb code 6hv8). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Cryo-Em Structure of S. Cerevisiae Polymerase Epsilon Deltacat Mutant, PDB code: 6hv8:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Cryo-Em Structure of S. Cerevisiae Polymerase Epsilon Deltacat Mutant


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Cryo-Em Structure of S. Cerevisiae Polymerase Epsilon Deltacat Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn2301 b:0.3 occ:1.00 SG A:CYS2108 2.2 0.8 1.0 SG A:CYS2111 2.2 0.3 1.0 SG A:CYS2133 2.2 0.6 1.0 SG A:CYS2130 2.3 0.7 1.0 CB A:CYS2130 2.3 0.7 1.0 CB A:CYS2111 2.7 0.3 1.0 CB A:CYS2108 3.5 0.8 1.0 N A:CYS2111 3.7 0.3 1.0 CB A:CYS2133 3.7 0.6 1.0 CA A:CYS2111 3.8 0.3 1.0 CA A:CYS2130 3.9 0.7 1.0 N A:CYS2133 3.9 0.6 1.0 CA A:CYS2133 4.2 0.6 1.0 C A:CYS2133 4.4 0.6 1.0 O A:CYS2133 4.5 0.6 1.0 N A:CYS2130 4.6 0.7 1.0 C A:CYS2130 4.6 0.7 1.0 C A:CYS2111 4.8 0.3 1.0 CA A:CYS2108 4.8 0.8 1.0 N A:VAL2131 4.9 99.6 1.0 CZ A:PHE2137 4.9 88.2 1.0 C A:TYR2110 4.9 0.8 1.0

Zinc binding site 2 out of 2 in the Cryo-Em Structure of S. Cerevisiae Polymerase Epsilon Deltacat Mutant


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Cryo-Em Structure of S. Cerevisiae Polymerase Epsilon Deltacat Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn2302 b:0.6 occ:1.00 N A:CYS2167 1.5 0.4 1.0 CB A:ARG2166 2.3 0.3 1.0 C A:ARG2166 2.3 0.3 1.0 SG A:CYS2181 2.4 0.4 1.0 SG A:CYS2164 2.5 0.5 1.0 SG A:CYS2167 2.5 0.4 1.0 CA A:ARG2166 2.6 0.3 1.0 CA A:CYS2167 2.6 0.4 1.0 SG A:CYS2179 2.7 0.4 1.0 CB A:CYS2167 2.8 0.4 1.0 N A:ARG2166 3.0 0.3 1.0 CG A:ARG2166 3.1 0.3 1.0 O A:ARG2166 3.5 0.3 1.0 C A:CYS2167 3.5 0.4 1.0 CB A:CYS2164 3.6 0.5 1.0 O A:CYS2167 3.6 0.4 1.0 CD A:ARG2166 4.0 0.3 1.0 CB A:CYS2179 4.0 0.4 1.0 CB A:CYS2181 4.0 0.4 1.0 C A:SER2165 4.1 0.4 1.0 CA A:GLY2183 4.4 0.2 1.0 N A:SER2165 4.5 0.4 1.0 N A:GLY2183 4.5 0.2 1.0 O A:SER2165 4.7 0.4 1.0 C A:CYS2164 4.7 0.5 1.0 CA A:CYS2164 4.8 0.5 1.0 N A:HIS2168 4.8 0.6 1.0 CA A:SER2165 4.9 0.4 1.0 NE A:ARG2166 5.0 0.3 1.0

P.Goswami, F.Abid Ali, M.E.Douglas, J.Locke, A.Purkiss, A.Janska, P.Eickhoff, A.Early, A.Nans, A.M.C.Cheung, J.F.X.Diffley, A.Costa. Structure of Dna-Cmg-Pol Epsilon Elucidates the Roles of the Non-Catalytic Polymerase Modules in the Eukaryotic Replisome. Nat Commun V. 9 5061 2018.
ISSN: ESSN 2041-1723
PubMed: 30498216
DOI: 10.1038/S41467-018-07417-1