Zinc in PDB 6fea: A. Vinelandii Vanadium Nitrogenase, Turnover State

All present enzymatic activity of A. Vinelandii Vanadium Nitrogenase, Turnover State:
1.18.6.1;

The structure of A. Vinelandii Vanadium Nitrogenase, Turnover State, PDB code: 6fea was solved by D.Sippel, O.Einsle, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	102.12 / 1.20
Space group	P 1
Cell size a, b, c (Å), α, β, γ (°)	75.610, 79.750, 107.160, 84.05, 72.44, 75.25
R / Rfree (%)	11.7 / 14.5

The structure of A. Vinelandii Vanadium Nitrogenase, Turnover State also contains other interesting chemical elements:

Magnesium	(Mg)	4 atoms
Vanadium	(V)	2 atoms
Iron	(Fe)	32 atoms

The binding sites of Zinc atom in the A. Vinelandii Vanadium Nitrogenase, Turnover State (pdb code 6fea). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the A. Vinelandii Vanadium Nitrogenase, Turnover State, PDB code: 6fea:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the A. Vinelandii Vanadium Nitrogenase, Turnover State


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of A. Vinelandii Vanadium Nitrogenase, Turnover State within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn505 b:16.0 occ:0.50 CE1 A:HIS448 2.0 12.9 0.5 NE2 E:HIS379 2.0 16.4 0.5 O E:HOH1032 2.0 32.1 1.0 O E:HOH1045 2.2 25.8 1.0 NE2 A:HIS448 2.7 20.3 0.5 CD2 E:HIS379 3.0 16.4 0.5 CE1 E:HIS379 3.0 19.6 0.5 ND1 A:HIS448 3.2 19.8 0.5 CD2 A:HIS448 3.9 15.7 0.5 ND1 E:HIS379 4.2 18.3 0.5 CG E:HIS379 4.2 13.8 0.5 CG A:HIS448 4.2 12.5 0.5 O A:HOH658 4.2 31.1 1.0 OH A:TYR444 4.6 20.1 1.0 O E:HOH1131 4.9 45.8 1.0

Zinc binding site 2 out of 2 in the A. Vinelandii Vanadium Nitrogenase, Turnover State


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of A. Vinelandii Vanadium Nitrogenase, Turnover State within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn503 b:23.0 occ:0.50 O B:HOH607 1.3 63.1 1.0 NE2 B:HIS379 2.1 18.9 0.5 NE2 D:HIS448 2.1 20.6 0.5 O D:HOH1043 2.2 36.6 1.0 CE1 D:HIS448 2.3 29.0 0.5 CE1 B:HIS379 3.0 24.2 0.5 CD2 B:HIS379 3.1 19.6 0.5 CD2 D:HIS448 3.4 29.6 0.5 O B:HOH1078 3.6 56.5 1.0 ND1 D:HIS448 3.6 26.6 0.5 CG D:HIS448 4.2 17.9 0.5 ND1 B:HIS379 4.2 24.2 0.5 CG B:HIS379 4.2 20.6 0.5 O D:HOH672 4.2 30.6 1.0 O D:HOH618 4.4 50.6 1.0 OH D:TYR444 4.7 19.9 1.0

D.Sippel, M.Rohde, J.Netzer, C.Trncik, J.Gies, K.Grunau, I.Djurdjevic, L.Decamps, S.L.A.Andrade, O.Einsle. A Bound Reaction Intermediate Sheds Light on the Mechanism of Nitrogenase. Science V. 359 1484 2018.
ISSN: ESSN 1095-9203
PubMed: 29599235
DOI: 10.1126/SCIENCE.AAR2765