Zinc in PDB 6e27: The CARD9 Card Domain-Swapped Dimer with A Zinc Ion Bound to One of the Two Zinc Binding Sites

The structure of The CARD9 Card Domain-Swapped Dimer with A Zinc Ion Bound to One of the Two Zinc Binding Sites, PDB code: 6e27 was solved by M.J.Holliday, R.Ferrao, G.Boenig, E.C.Deuber, W.J.Fairbrother, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	43.08 / 1.81
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	44.010, 37.460, 56.960, 90.00, 101.79, 90.00
R / Rfree (%)	20.7 / 25.8

The binding sites of Zinc atom in the The CARD9 Card Domain-Swapped Dimer with A Zinc Ion Bound to One of the Two Zinc Binding Sites (pdb code 6e27). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total only one binding site of Zinc was determined in the The CARD9 Card Domain-Swapped Dimer with A Zinc Ion Bound to One of the Two Zinc Binding Sites, PDB code: 6e27:

Zinc binding site 1 out of 1 in the The CARD9 Card Domain-Swapped Dimer with A Zinc Ion Bound to One of the Two Zinc Binding Sites


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of The CARD9 Card Domain-Swapped Dimer with A Zinc Ion Bound to One of the Two Zinc Binding Sites within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn101 b:63.8 occ:1.00 ND1 C:HIS73 1.9 95.2 1.0 SG D:CYS10 2.3 90.1 1.0 O D:HOH129 2.6 50.6 1.0 HB2 D:CYS10 2.7 68.6 1.0 CE1 C:HIS73 2.8 73.2 1.0 HE1 C:HIS73 2.9 87.8 1.0 CG C:HIS73 3.0 70.3 1.0 CB D:CYS10 3.0 57.1 1.0 HB2 C:HIS73 3.1 47.2 1.0 H D:CYS10 3.4 52.1 1.0 CB C:HIS73 3.5 39.3 1.0 HB3 D:CYS10 3.6 68.6 1.0 HA C:HIS73 3.7 41.9 1.0 NE2 C:HIS73 3.9 69.3 1.0 CD2 C:HIS73 4.1 56.2 1.0 N D:CYS10 4.1 43.4 1.0 CA C:HIS73 4.2 35.0 1.0 CA D:CYS10 4.2 41.9 1.0 HB3 C:HIS73 4.4 47.2 1.0 HA D:CYS10 4.7 50.3 1.0 HE2 C:HIS73 4.7 83.2 1.0 H D:GLU9 4.7 72.1 1.0 HD2 C:TYR76 4.7 42.5 1.0 HB3 C:TYR76 4.8 43.8 1.0 HD2 C:HIS73 4.9 67.4 1.0 N C:HIS73 5.0 41.7 1.0

M.J.Holliday, R.Ferrao, G.De Leon Boenig, A.Estevez, E.Helgason, A.Rohou, E.C.Dueber, W.J.Fairbrother. Picomolar Zinc Binding Modulates Formation of BCL10-Nucleating Assemblies of the Caspase Recruitment Domain (Card) of CARD9. J. Biol. Chem. V. 293 16803 2018.
ISSN: ESSN 1083-351X
PubMed: 30206119
DOI: 10.1074/JBC.RA118.004821