Zinc in PDB 6cce: Crystal Structure of A Mycobacterium Smegmatis Rna Polymerase Transcription Initiation Complex with Inhibitor Kanglemycin A

Enzymatic activity of Crystal Structure of A Mycobacterium Smegmatis Rna Polymerase Transcription Initiation Complex with Inhibitor Kanglemycin A

All present enzymatic activity of Crystal Structure of A Mycobacterium Smegmatis Rna Polymerase Transcription Initiation Complex with Inhibitor Kanglemycin A:
2.7.7.6;

Protein crystallography data

The structure of Crystal Structure of A Mycobacterium Smegmatis Rna Polymerase Transcription Initiation Complex with Inhibitor Kanglemycin A, PDB code: 6cce was solved by M.Lilic, S.A.Darst, E.A.Campbell, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 49.55 / 3.05
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 130.564, 161.583, 135.141, 90.00, 110.73, 90.00
R / Rfree (%) 23.5 / 26.5

Other elements in 6cce:

The structure of Crystal Structure of A Mycobacterium Smegmatis Rna Polymerase Transcription Initiation Complex with Inhibitor Kanglemycin A also contains other interesting chemical elements:

Magnesium (Mg) 1 atom

Zinc Binding Sites:

The binding sites of Zinc atom in the Crystal Structure of A Mycobacterium Smegmatis Rna Polymerase Transcription Initiation Complex with Inhibitor Kanglemycin A (pdb code 6cce). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total only one binding site of Zinc was determined in the Crystal Structure of A Mycobacterium Smegmatis Rna Polymerase Transcription Initiation Complex with Inhibitor Kanglemycin A, PDB code: 6cce:

Zinc binding site 1 out of 1 in 6cce

Go back to Zinc Binding Sites List in 6cce
Zinc binding site 1 out of 1 in the Crystal Structure of A Mycobacterium Smegmatis Rna Polymerase Transcription Initiation Complex with Inhibitor Kanglemycin A


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of A Mycobacterium Smegmatis Rna Polymerase Transcription Initiation Complex with Inhibitor Kanglemycin A within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Zn2001

b:1.0
occ:1.00
SG D:CYS977 2.2 95.1 1.0
SG D:CYS967 2.3 75.4 1.0
SG D:CYS890 2.3 95.2 1.0
SG D:CYS974 2.3 86.9 1.0
CB D:CYS967 3.1 89.7 1.0
CB D:CYS977 3.1 91.7 1.0
CA D:CYS967 3.4 0.4 1.0
CB D:CYS890 3.5 93.6 1.0
NH1 D:ARG962 3.5 89.2 1.0
CB D:CYS974 3.7 87.6 1.0
OG D:SER969 3.9 0.3 1.0
N D:THR968 4.0 0.4 1.0
C D:CYS967 4.1 100.0 1.0
CG2 D:THR892 4.3 0.5 1.0
N D:CYS890 4.3 91.9 1.0
CA D:CYS977 4.3 83.6 1.0
N D:CYS974 4.4 75.3 1.0
N D:CYS977 4.4 67.9 1.0
CA D:CYS890 4.5 95.6 1.0
CA D:CYS974 4.6 83.2 1.0
CZ D:ARG962 4.6 69.0 1.0
N D:CYS967 4.6 87.5 1.0
N D:SER969 4.7 95.3 1.0
NH2 D:ARG962 5.0 95.8 1.0

Reference:

J.Peek, M.Lilic, D.Montiel, A.Milshteyn, I.Woodworth, J.B.Biggins, M.A.Ternei, P.Y.Calle, M.Danziger, T.Warrier, K.Saito, N.Braffman, A.Fay, M.S.Glickman, S.A.Darst, E.A.Campbell, S.F.Brady. Rifamycin Congeners Kanglemycins Are Active Against Rifampicin-Resistant Bacteria Via A Distinct Mechanism. Nat Commun V. 9 4147 2018.
ISSN: ESSN 2041-1723
PubMed: 30297823
DOI: 10.1038/S41467-018-06587-2
Page generated: Wed Dec 16 11:35:48 2020

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